1. Serum albumin modified by carbamoylation impairs macrophage cholesterol efflux in diabetic kidney disease
- Author
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Márcia Silva Queiroz, R. S. Pinto, Maria Lúcia Corrêa-Giannella, Aécio Lopes de Araújo Lira, Monique de Fátima Mello Santana, Carlos André Minanni, Rodrigo T. Iborra, Marisa Passarelli, and Adriana Machado Saldiba de Lima
- Subjects
medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Serum albumin ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Glycation ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Humans ,Diabetic Nephropathies ,Uremic Toxins ,Pentosidine ,Renal Insufficiency, Chronic ,Serum Albumin ,Homocitrulline ,Protein Carbamylation ,biology ,Cholesterol ,business.industry ,Macrophages ,Reverse cholesterol transport ,Albumin ,medicine.disease ,chemistry ,Diabetes Mellitus, Type 2 ,biology.protein ,business - Abstract
Background and aims Abnormalities in lipid metabolism, accumulation of uremic toxins and advanced glycation end products may contribute to worsening atherosclerosis. This study characterized the glycation and carbamoylation profile of serum albumin isolated from individuals with diabetic kidney disease and its influence on cholesterol efflux. Material and methods 49 patients with type 2 diabetes (T2DM) and different eGFR evaluated glycation and carbamoylation profile by measurement of carboxymethyl lysine (CML) and carbamoylated proteins (CBL) in plasma by ELISA, homocitrulline (HCit) in plasma by colorimetry. In the isolated albumins, we quantified CBL (ELISA) and total AGE and pentosidine by fluorescence. Macrophages were treated with albumin isolated, and 14C-Cholesterol efflux mediated by HDL2 or HDL3 was measured. Kruskal-Wallis test, Jonckheere-Terpstra test and Brunner's posttest were used for comparisons among groups. Results Determination of CML, HCit, CBL in plasma, as total AGE and pentosidine in albumins, did not differ between groups; however, CBL in the isolated albumins was higher in the more advanced stages of CKD (p = 0.0414). There was reduction in the 14C-cholesterol efflux after treatment for 18 h with albumin isolated from patients with eGFR Conclusions Albumins isolated from individuals with T2DM and eGFR
- Published
- 2020