1. Gadolinium-conjugated PLA-PEG nanoparticles as liver targeted molecular MRI contrast agent.
- Author
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Chen Z, Yu D, Liu C, Yang X, Zhang N, Ma C, Song J, and Lu Z
- Subjects
- Animals, Biocompatible Materials chemistry, Cell Survival drug effects, Chromatography, Gel, Contrast Media administration & dosage, Contrast Media pharmacokinetics, Contrast Media toxicity, Drug Carriers chemical synthesis, Gadolinium DTPA administration & dosage, Gadolinium DTPA pharmacokinetics, Gadolinium DTPA toxicity, Hep G2 Cells, Humans, Lactates chemical synthesis, Light, Microscopy, Electron, Transmission, Models, Molecular, Molecular Imaging, Molecular Structure, Particle Size, Polyethylene Glycols chemical synthesis, Rats, Rats, Wistar, Scattering, Radiation, Surface Properties, Tissue Distribution, Contrast Media chemistry, Drug Carriers chemistry, Gadolinium chemistry, Gadolinium DTPA chemistry, Lactates chemistry, Liver metabolism, Magnetic Resonance Imaging, Nanoparticles chemistry, Polyethylene Glycols chemistry
- Abstract
A nanoparticle magnetic resonance imaging (MRI) contrast agent targeted to liver was developed by conjugation of gadolinium (Gd) chelate groups onto the biocompatible poly(l-lactide)-block-poly (ethylene glycol) (PLA-PEG) nanoparticles. PLA-PEG conjugated with diethylenetriaminopentaacetic acid (DTPA) was used to formulate PLA-PEG-DTPA nanoparticles by solvent diffusion method, and then Gd was loaded onto the nanoparticles by chelated with the unfolding DTPA on the surface of the PLA-PEG-DTPA nanoparticles. The mean size of the nanoparticles was 265.9 ± 6.7 nm. The relaxivity of the Gd-labeled nanoparticles was measured, and the distribution in vivo was evaluated in rats. Compared with conventional contrast agent (Magnevist), the Gd-labeled PLA-PEG nanoparticles showed significant enhancement both on liver targeting ability and imaging signal intensity. The T(1) and T(2) relaxivities per [Gd] of the Gd-labeled nanoparticles was 18.865 mM(-1) s(-1) and 24.863 mM(-1) s(-1) at 3 T, respectively. In addition, the signal intensity in vivo was stronger comparing with the Gd-DTPA and the T(1) weight time was lasting for 4.5 h. The liver targeting efficiency of the Gd-labeled PLA-PEG nanoparticles in rats was 14.57 comparing with Magnevist injection. Therefore, the Gd-labeled nanoparticles showed the potential as targeting molecular MRI contrast agent for further clinical utilization.
- Published
- 2011
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