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We examined the availability and reliability of surface electromyography (EMG) signals from the iliopsoas muscle (IL). Using serial magnetic resonance images from fifty healthy young males, we evaluated whether the superficial region of IL was adequate for attaching surface EMG electrodes. Subsequently, we assessed EMG cross-talk from the sartorius muscle (SA)-the nearest to IL-using a selective cooling method in fourteen subjects. The skin above SA was cooled, and the median frequencies of EMG signals from IL and SA were determined. The maximum voluntary contraction during isometric hip flexion was measured before and after selective cooling, and surface EMG signals from SA and IL were measured. The superficial area of IL was adequately large (13.2±2.7cm(2)) for recording surface EMG in all fifty subjects. The maximum perimeter for the medial-lateral skin facing IL was noted at a level 3-5cm distal to the anterior superior iliac spine. Following cooling, the median frequency for SA decreased significantly (from 70.1 to 51.9Hz, p0.001); however, that for IL did not alter significantly. These results demonstrated that EMG cross-talk from SA was negligible for surface EMG signals from IL during hip flexion.