1. Interleukin-1beta (IL-1beta) and IL-6 modulate insulin-like growth factor-binding protein (IGFBP) secretion in colon cancer epithelial (Caco-2) cells
- Author
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Ian R. Sanderson, Cecilia Camacho-Hübner, S Bernasconi, G Giovannelli, Maria E. Street, Martin O. Savage, and Farideh Miraki-Moud
- Subjects
medicine.medical_specialty ,Colon ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Insulin-like growth factor-binding protein ,Endocrinology ,Carcinoembryonic antigen ,Internal medicine ,medicine ,Humans ,Dose-Response Relationship, Drug ,biology ,Interleukin-6 ,Cell growth ,Chemistry ,Interleukins ,Interleukin ,Cell Differentiation ,DNA ,Insulin-Like Growth Factor Binding Proteins ,Drug Combinations ,Insulin-Like Growth Factor Binding Protein 2 ,Dose–response relationship ,Cytokine ,Insulin-Like Growth Factor Binding Protein 4 ,Caco-2 ,Cell culture ,Protein Biosynthesis ,biology.protein ,Caco-2 Cells ,Cell Division ,Interleukin-1 - Abstract
Chronic inflammation is characterised by modifications in cytokine concentrations, whereas growth is mainly dependent on the GH-IGF axis. IGF-I bioavailability is modulated by a family of IGF-binding proteins (IGFBPs). The aim of the present study was to evaluate the interactions among interleukin-1beta (IL-1beta), IL-6 and IGFBP secretion by intestinal cells to assess whether cytokines modulate IGFBP secretion, and in turn IGF-I and IGF-II bioavailability. The human colon carcinoma derived cell line Caco-2 was used as an in vitro model for its capacity to differentiate spontaneously. Experiments were carried out on day 4 (undifferentiated state) and day 14 (differentiated state) after plating. Carcinoembryonic antigen (CEA) was used as a marker of differentiation and increased in the conditioned media (CM) from days 4 to 14 (0.2+/-0.01 ng/ml per 10(5) cells vs 3.3+/-0.2 ng/ml per 10(5) cells, P
- Published
- 2003
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