1. Role of neutrophil extracellular traps in chronic kidney injury induced by bisphenol-A
- Author
-
Renzhi Hu, Qianna Zhen, Xuan Li, Rufei Gao, Shiyun Tong, Ting Luo, Ting Li, Qifu Li, Yi Yang, Jinbo Hu, Chuan Peng, Shumin Yang, and Hua Qing
- Subjects
0301 basic medicine ,endocrine system ,medicine.medical_specialty ,Proteases ,Endocrinology, Diabetes and Metabolism ,030232 urology & nephrology ,Podocyte ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,In vivo ,Internal medicine ,medicine ,Fibroblast ,Kidney ,Proteinuria ,urogenital system ,business.industry ,Neutrophil extracellular traps ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,medicine.symptom ,business ,Nephritis ,hormones, hormone substitutes, and hormone antagonists - Abstract
Bisphenol-A (BPA) is a common environmental pollutant, and exposure to it is associated with proteinuria and may predict the progression of chronic kidney disease; however, the mechanism is not clear. Neutrophil extracellular traps (NETs) are a DNA skeleton coated with various proteases, and it is associated with various types of autoimmune nephritis. In this study, we examine whether NETs is involved in BPA-induced chronic kidney injury. In vivo, BPA exposure resulted in impaired renal function and altered renal morphology, including glomerular mesangial matrix expansion and increased renal interstitial fibroblast markers. Meanwhile, more dsDNA can be detected in the serum, and the NETs-associated proteins, MPO and citH3 were deposited in the renal system. In vitro, BPA and NETs treatment caused podocyte injury, a loss of marker proteins and disorder in the actin skeleton. After NETs inhibition via DNase administration, BPA-induced injuries were significantly relieved. In conclusion, the increase of NETosis in circulation and the renal system during BPA exposure suggests that NETs may be involved in BPA-induced chronic kidney injury.
- Published
- 2019