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Bacteria play a variety of roles in the environment. They maintain the balance in the ecosystem and provide different ecosystem services such as in biogeochemical cycling of nutrients, biodegradation of toxic pollutants, and others. Therefore, isolation and identification of different environmental bacteria are important to most environmental research. Due to the high cost and time associated with the conventional molecular techniques, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has gained considerable attention for routine identification of bacteria. This review aims to provide an overview of the application of MALDI-TOF MS in various environmental studies through bibliometric analysis and literature review. The bibliometric analysis helped to understand the time-variable application of MALDI-TOF MS in various environmental studies. The categorical literature review covers various environmental studies comprising areas like ecology, food microbiology, environmental biotechnology, agriculture, and plant sciences, which show the application of the technique for identification and characterization of pollutant-degrading, plant-associated, disease-causing, soil-beneficial, and other environmental bacteria. Further research should focus on bridging the gap between the phylogenetic identity of bacteria and their specific environmental functions or metabolic traits that can help in rapid advancements in environmental research, thereby, improving time and cost savings., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Abstract In this study, ten bacterial strains were found to be mercury resistant after their isolation from Qatari coastal sediments. Tolerance was found to be up to 100–150 ppm for five strains. Those strains had optimum growth conditions at salinity level of 10 ppm NaCl and pH 7–8. Starting from a concentration 7.9 ppm of mercury extracted from fluorescent lamps and after 6 days of incubation at 37 °C, two isolated strains HA6 (Bacillus spp.) and HA9 (Acinetobacter sp.) showed 96.7% and 98.9% of mercury bio-uptake efficiency, respectively. Other strains were capable of removing more than 60% of extracted mercury. Highlights • Bacillus spp. and Acinetobacter sp. showed 96.7% and 98.9% Hg removal. • Other strains were capable of removing more than 60% of extracted mercury. • Almost all strain exhibited the highest growth density at pH level between 7 and 8. • Acinetobacter sp. was able to be tolerant up to 200 ppm of mercury. • Such strains can be applied in bioremediation of highly mercury-polluted areas. [ABSTRACT FROM AUTHOR]