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Excessive organic matter in the anaerobic ammonia oxidation (Anammox) leads to the growth of a large number of heterotrophic bacteria, which disrupts the anaerobic ammonia oxidation. The adsorption-anaerobic ammonia oxidation process can effectively reduce excessive organic matter, capturing it instead of consuming it, which is a sustainable development technology. In this study, utilizing the excellent adsorption performance of aerobic granular sludge (AGS), an adsorption-regeneration process was employed to remove organic matter at the front end of the Anammox process through bio-adsorption in an artificial simulated domestic sewage environment, and it was successfully used for denitrification. Stirring rate is a key factor affecting sludge granulation. As a parallel experiment of sludge granulation, two Sequencing Batch Reactors (SBRs) (R1 and R2) were operated simultaneously at different stirring rates. After 153 days, the particle size of the two reactors was analyzed, revealing that the proportion of particles larger than 200 μm was over 50%, and granular sludge was successfully formed in both reactors. Long-term operational results indicate that at a temperature of 16.5 ± 1 °C, varying initial pH levels (6.5, 6.7, 7.2, and 8.5) significantly affect the removal efficiency of chemical oxygen demand (COD). COD is rapidly adsorbed and removed within a short period. Among the tested initial pH values, a pH of 6.7 yielded the best total chemical oxygen demand (tCOD) removal efficiency, achieving up to 95%. Additionally, the study examined the effects of different carbon sources on denitrification, revealing that under carbon-rich conditions, the denitrification rate was highest, reaching 1.44 mg N/(g VSS·h). Compared to endogenous denitrification, the denitrification rate increased by 40%, and the nitrate (NO₃⁻-N) removal efficiency reached 100%. • Granular sludge formation enhances the bio-adsorption of organic matter. • Lower initial pH facilitates rapid bio-adsorption rates. • Carbon-rich sludge denitrification rate matches traditional methods. • Bio-adsorption mitigates the impact of organic matter on anaerobic ammonium oxidation. [ABSTRACT FROM AUTHOR]

One of the factors that has a direct impact on anaerobic digestion is the applied organic loading rate (OLR A). Increasing OLR A can boost methane production but can also cause process failure. As a result, establishing the appropriate OLR A for the procedure is critical. This study evaluated the effect of increasing the OLR A using soybean molasses in a thermophilic anaerobic reactor (R-Thermo), as well as the effect of feeding strategy and co-processing with okara. Furthermore, the performance versus stability trade-off between R-Thermo and mesophilic anaerobic digestion (R-Meso) was investigated. The increase of OLR A from 10 to 15 and 20 kg-COD/m³/d led to a decrease in COD removal efficiency (90, 86, and 75%), methane yield (12.0, 11.6, and 9.9 mol-CH 4 /kg-COD) and an increase in total volatile acids concentration (251, 456, and 1393 mg-HAc/L, respectively). At 15 kg-COD/m³/d, R-Meso performed similarly to R-Thermo, and at 20 kg-COD/m3/d, R-Meso outperformed (81% COD removal efficiency, 9.3 mol-CH 4 /kg-COD rem and 154.5 mol-CH 4 /m3/d). Temperature greatly influenced the distribution of metabolic pathways, as shown by thermodynamic and kinetic analyses, thus impacting bacterial diversity. At 55 °C, amongst the bacterial genera, Tepidiphilus stood out (>28.2%), followed by Acetomicrobium , Coprothermobacter and Candidatus_Caldatribacterium. The OLR A clearly impacted the archaeal community; Methanothermobacter (77.4%) was favored over Methanosarcina (14.8%). Under thermophilic temperature, it seems that syntrophic acetate oxidation (SAO) bacteria might have competed for substrate with acetoclastic methanogens, while in R-Meso microorganisms responsible for the initial steps of organic matter breakdown, such as members of the Firmicutes and Proteobacteria phyla (at least 67%), were dominant. In summary, R-Meso, characterized by a more uniform distribution of metabolic pathways, as well as a diverse and well-adapted microbial consortium, have exhibited enhanced stability and outperformed R-Thermo at high-loads. [Display omitted] • Thermophilic and mesophilic digestion of soybean molasses was compared. • R-Meso and R-Thermo showed similar COD removal and CH 4 production at different loads. • R-Meso digestion resulted in balanced production of intermediate metabolites. • Less diversity in the microbiological consortium present in R-Thermo. • Competitiveness between SAO and acetoclastic methanogens at high temperature. [ABSTRACT FROM AUTHOR]

The impact of a novel sawdust-modified carrier on the performance of aerobic sequencing batch reactor (SBR) was examined. Compared with the conventional polyethylene (PE) carrier, the sawdust-modified carrier had coarse surface and porous side wall, which was beneficial for the rapid formation of biofilm. The biomass of sawdust-modified carrier was 3.4 ± 0.7 times more than those of PE carrier at the end of this study. The biofilm gotten from suspended carrier had higher extracellular polymeric substances (EPS) concentrations than activated sludge (AS). The EPS from biofilm contained higher proportions of polysaccharides compared to those from AS. The SBR with addition of sawdust-modified carrier exhibited higher ammonia nitrogen removal efficiency (84.8%) than the one with addition of conventional PE carrier (73.1%) in a typical cycle at 12 h. The volumetric nitrification rates of modified carrier were higher than those of conventional PE carrier. High throughput sequencing revealed that sawdust-modified carriers exhibited greater microbial richness and diversity compared with traditional PE carriers. Saccharimonadales was the most predominant genus that removed organic matter under aerobic condition, whereas Nitrospira was the dominant nitrifying genus. The present study verifies the advantage of sawdust-modified carrier, which has the potential for the full-scale application in the future. [Display omitted] • A novel sawdust-modified carrier with coarse surface and porous side wall was made. • The biomass of novel carrier was 3.4 ± 0.7 times more than those of PE carrier. • Nitrification rate of novel carrier was 3.16 ± 0.64 times than that of PE carrier. • Novel carrier improved the organic matter removal and nitrification ability of SBR. • Novel carrier had higher microbial richness and diversity than common PE carrier. [ABSTRACT FROM AUTHOR]

The algae–bacterial granular sludge (ABGS) technology has garnered significant attention due to its remarkable attributes of low carbon emissions. To investigate the performance of the ABGS system under various substrate loading rates, the parallel photo-sequencing batch reactors (P1 and P2) were set up. The results indicated that chlorophyll-a content and extracellular polymeric substance content were measured at 10.7 ± 0.3 mg/L and 61.4 ± 0.7 mg/g SS in P1 under relatively low substrate loading rate (0.9 kg COD/m3/d and 0.09 kg N/m3/d). Moreover, kinetic study revealed that the maximal specific P uptake rate for P1 reached 0.21 mg P/g SS/h under light conditions, and it achieved 0.078 mg P/g SS/h under dark conditions, highlighting the significant role on phosphorus removal played by algae in the ABGS system. The microbial analysis and scanning electron microscopy confirmed that filamentous algae predominantly colonize the surface in P1, whereas spherical bacteria dominate the surface of granular sludge in P2. Additionally, a diverse array of microorganisms including bacteria, algae, and metazoa such as Rotifers and Nematodes were observed in both systems, providing evidence for the establishment of a symbiotic system. This study not only confirmed the ability of ABGS for efficient N and P removal under different substrate loading conditions but also highlighted its potential to enhance the ecological diversity of the reaction system. [Display omitted] • Algal–bacterial granular sludge was successfully cultured under various substrate loading rates. • Under a low substrate loading rate, Leptolyngbya PCC-6306 (35.6%) became the dominant bacterial species of ABGS. • Phosphorus removal was more effective under light conditions in kinetic experiments. • A diverse microbial symbiosis was observed in the ABGS system under a low substrate loading rate. [ABSTRACT FROM AUTHOR]

The positive contributions of carriers to aerobic granulation have been wildly appreciated. In this study, as a way resource utilization, the dredged sediment was thermally-treated to prepared as carriers to promote aerobic granular sludge (AGS) formation and stability. The system was started under low superficial gas velocity (SGV, 0.6 cm/s)for a lower energy consumption. Two sequencing batch reactors (SBR) labeled R1 (no added carriers) and R2 (carriers added), were used in the experiment. R2 had excellent performance of granulation time (shortened nearly 43%). The maximum mean particle size at the maturity stage of AGS in R2 (0.545 mm) was larger compared to R1 (0.296 mm). The sludge settling performance in R2 was better. The reactors exhibited high chemical oxygen demand (COD) and ammonia nitrogen (NH 3 -N) removal rates. The total phosphorus (TP) removal rate in R2 was higher than R1 (almost 15% higher) on stage II (93-175d). R2 had a higher microbial abundance and dominant bacteria content. The relative abundance of dominant species was mainly affected by the carrier. However, the enrichment of dominant microorganisms and the evolution of subdominant species were more influenced by the increase of SGV. The results indicated that the addition of carriers induced the secretion of extracellular polymeric substances (EPS) by microorganisms and accelerated the rapid formation of initial microbial aggregates. This work provided a low-cost method and condition to enhance aerobic granulation, which may be helpful in optimizing wastewater treatment processes. [Display omitted] • The AGS system was successfully started under low SGV (0.6 cm/s). • Thermally-treated dredged sediment was used as a carrier for resource utilization. • The carriers stimulated the secretion of EPS, shortening aerobic granulation. • Enhanced AGS exhibited greater particle size and higher microbial abundance. • The TP removal effect of the enhanced AGS system has been improved. [ABSTRACT FROM AUTHOR]

Biological treatment represents a fundamental component of wastewater treatment plants (WWTPs). The transmission of antibiotic resistance bacteria (ARB) and resistance genes (ARGs) occurred through the continuous migration and transformation, attributed to the residual presence of antibiotics in WWTPs effluent, posing a significant threat to the entire ecosystem. It is necessary to propose novel biological strategies to address the challenge of refractory contaminants, such as antibiotics, ARGs and ARB. This review summarizes the occurrence of antibiotics in wastewater, categorized by high and low concentrations. Additionally, current biological treatments used in WWTPs, such as aerobic activated sludge, anaerobic digestion, sequencing batch reactor (SBR), constructed wetland, membrane-related bioreactors and biological aerated filter (BAF) are introduced. In particular, because microorganisms are the key to those biological treatments, the effect of high and low concentration of antibiotics on microorganisms are thoroughly discussed. Finally, solutions involving functional bacteria, partial nitrification (PN)-Anammox and lysozyme embedding are suggested from the perspective of the entire biological treatment process. Overall, this review provides valuable insights for the simultaneous removal of antibiotics and ARGs in antibiotics wastewater. [Display omitted] • It roughly divided antibiotics wastewater into two types according concentration. • Dual effects of antibiotics on microorganisms were discussed. • Partial antibiotics resistant bacteria were used in antibiotics removing. • Anammox process might be used for removing antibiotics in biological treatment. • Lysozyme embedding technology had potential for intercepting resistant genes. [ABSTRACT FROM AUTHOR]

Granular sludge is an alternative technology for the direct treatment of acidic nitrate-containing wastewater. Rapid remediation of disintegrated granules is essential to achieve efficient nitrogen removal. In this study, denitrifying granules were inactivated and disintegrated when the influent nitrate-nitrogen concentration was elevated from 240 to 360 mg L−1 in acidic wastewater (pH = 4.1) in a sequencing batch reactor. Tightly bound extracellular polymeric substances (TB-EPS) decreased by 60%, and extracellular protein (PN) was the main component of the reduced EPS. The three-dimensional excitation emission matrices (3D-EEM) results confirmed that the PNs that decreased were mainly tryptophan-like, tyrosine-like, and aromatic. This study further confirmed that the decrease in PN was mainly from the destruction of C=O (amide I) and N–H functional groups. Overloading of nitrogen-inhibited denitrifying activity and the destruction and dissolution of TB-EPS by acidic pH were responsible for granule disintegration, with PNs playing a major role in maintaining granule stability. Based on this, new granules with an average particle size of 454.4 μm were formed after calcium chloride addition; EPS nearly doubled during granule formation with PN as the dominant component, accounting for 64.7–78.4% of the EPS. Atomic force microscopy (AFM) revealed that PN-PN adhesion increased by 1.6–4.9 times in the presence of calcium ions, accelerating the re-granulation of disintegrated particles. This study provides new insights into the disintegration and remediation of granular sludge under acidic conditions. [Display omitted] • Acidic pH destroyed C]O and N–H groups in EPS, disintegrating denitrifying granules. • PNs played a major role in maintaining granular sludge stability. • Rapid re-granulation was achieved by addition of CaCl 2 under acidic conditions. • Calcium ion addition increased protein-protein adhesion by 1.6–4.9 times. • The granular sludge remediation mechanism was elucidated from molecular forces. [ABSTRACT FROM AUTHOR]

Sequencing batch biofilm reactor (SBBR) has the potential to treat hypersaline high-strength nitrogen wastewater by simultaneous nitrification-denitrification (SND). Dissolved oxygen (DO) and aeration modes are major factors affecting pollutant removal. Low DO (0.35–3.5 mg/L) and alternative anoxic/aerobic (A/O) mode are commonly used for municipal wastewater treatment, however, the appropriate DO concentration and operation mode are still unknown under hypersaline environment because of the restricted oxygen transfer in denser extracellular polymeric substances (EPS) barrier and the decreased carbon source consumption during the anoxic phase. Herein, two SBBRs (R1, fully aerobic mode; R2, A/O mode) were used for the treatment of hypersaline high-strength nitrogen wastewater (200 mg/L NH 4 +-N, COD/N of 3 and 3% salinity). The results showed that the relatively low DO (2 mg/L) could not realize effective nitrification, while high DO (4.5 mg/L) evidently increased nitrification efficiency by enhancing oxygen transfer in denser biofilm that was stimulated by high salinity. A stable SND was reached 16 days faster with a ∼10% increase of TN removal under A/O mode. Mechanism analysis found that denser biofilm with coccus and bacillus were present in A/O mode instead of filamentous microorganisms, with the secretion of more EPS. Corynebacterium and Halomonas were the dominant genera in both SBBRs, and HN-AD process might assist partial nitrification-denitrification (PND) for highly efficient TN removal in biofilm systems. By using the appropriate operation mode and parameters, the average NH 4 +-N and TN removal efficiency could respectively reach 100% and 70.8% under the NLR of 0.2 kg N·m−3·d−1 (COD/N of 3), which was the highest among the published works using SND-based SBBRs in treatment of saline high-strength ammonia nitrogen (low COD/N) wastewater. This study provided new insights in biofilm under hypersaline stress and provided a solution for the treatment of hypersaline high-strength nitrogen (low COD/N) water. [Display omitted] • Hypersaline high-strength nitrogen (low COD/N) wastewater was treated by SBBRs. • High DO (4.5 mg/L) was needed to realize effective SND under hypersaline stress. • A/O mode shortened the start-up phase (16 days) and enhanced TN removal (∼10%). • HN-AD might assist PND for highly-efficient TN removal in low COD/N wastewater. • The highest TN removal (70.1%) was obtained in similar sewage treatment by SBBRs. [ABSTRACT FROM AUTHOR]

The disintegration and instability of aerobic granular sludge (AGS) systems during long-term operation pose significant challenges to its practical implementation, and rapid recovery strategies for disintegrated AGS are gaining more attention. In this study, the recovery and re-stabilization of disintegrated AGS was investigated by adding chitosan to a sequencing batch reactor and simultaneously adjusting the pH to slightly acidic condition. Within 7 days, chitosan addition under slight acidity led to the re-aggregation of disintegrated granules, increasing the average particle size from 166.4 μm to 485.9 μm. Notably, sludge volume indexes at 5 min (SVI 5) and 30 min (SVI 30) decreased remarkably from 404.6 mL/g and 215.1 mL/g (SVI 30 /SVI 5 = 0.53) to 49.1 mL/g and 47.6 mL/g (SVI 30 /SVI 5 = 0.97), respectively. Subsequent operation for 43 days successfully re-stabilized previous collapsed AGS system, resulting in an average particle size of 750.2 μm. These mature and re-stabilized granules exhibited characteristics of large particle size, excellent settleability, compact structure, and high biomass retention. Furthermore, chitosan facilitated the recovery of COD and nitrogen removal performances within 17–23 days of operation. It effectively facilitated the rapid aggregation of disintegrated granules by charge neutralization and bridging effects under a slightly acidic environment. Moreover, the precipitated chitosan acted as carriers, promoting the adhesion of microorganisms once pH control was discontinued. The results of batch tests and microbial community analysis confirmed that chitosan addition increased sludge retention time, enriching slow-growing microorganisms and enhancing the stability and pollutant removal efficiency of the AGS system. [Display omitted] • Chitosan addition achieved rapid recovery of disintegrated granules within 7 days. • Chitosan accelerated reaggregation of granules by charge neutralization and bridging. • Chitosan acted as carriers to promote microbial adhesion after precipitation. • The addition of chitosan avoids excessive loss of biomass in the system. • Slow-growing and functional microorganisms ensure the re-stabilization of system. [ABSTRACT FROM AUTHOR]

Operational mode and powdered activated carbon (PAC) are key factors facilitating microbial syntrophy and interspecies electron transfer during anaerobic digestion, consequently benefiting process stability and efficient methanogenesis. In this study, continuous-flow reactor (CFR) and sequencing batch reactor (SBR), with and without the addition of PAC, respectively, were operated to examine their effects on system performance and methanogenic activity. Based on the cycle-test result, the PAC-amended CFR (CFR PAC) recorded both the highest methane yield (690.1 mL/L) and the maximum CH 4 production rate (28.8 mL/(L·h)), while SBRs exhibited slow methanogenic rates. However, activity assays indicated that SBRs were beneficial for organics removal in batch experiments fed with peptone. Taxonomic and functional analysis confirmed that CFRs were optimal for proliferating oligotrophs (e.g., Geobacter) and SBRs were more suitable for copiotrophs (e.g., Desulfobulbus). Metagenomic analysis revealed that CFRs had efficient acetate metabolic pathways from propionate and ethanol, whereas SBRs did not, resulting in the buildup of propionate. Furthermore, Methanobacterium and Methanothrix were acclimated to the different operational conditions, while acetoclastic Methanosarcina and hydrogenotrophic Methanolinea were acclimated in SBRs (5.1–13.4%) and CFRs (0.3–1.7%), respectively. This study confirmed the enhancement of microbial syntrophy by the addition of PAC as well as the acclimation of electroactive bacteria (e.g., Geobacter) with complex organic substances. [Display omitted] • CFRs outperformed SBRs in substrate biodegradation and methanogenic activity. • The operational mode affected the metabolism of peptone and glucose differently. • Only CFRs had acetate metabolic pathways from propionate and ethanol by Geobacter. • PAC facilitated the acclimation of Methanothrix and Methanolinea in CFRs. • PAC promoted acetoclastic methanogenesis via the homoacetogenic pathway. [ABSTRACT FROM AUTHOR]

Sludge alkaline fermentation liquid (SAFL) is a promising alternative to acetate for improving biological nitrogen removal (BNR) from wastewater. SAFL inevitably contains some refractory compounds, while the characteristics of dissolved organic matter (DOM) in effluent from SAFL-fed BNR process remain unclear. In this study, the molecular weight distribution, fluorescent composition and molecular profiles of DOM in effluent from SAFL and acetate-fed sequencing batch reactors (S-SBRs and A-SBRs, respectively) at different hydraulic retention time (12 h and 24 h) was comparatively investigated. Two carbon sources resulted in similar effluent TN, but a larger amount of DOM, which was bio-refractory or microorganisms-derived, was found in effluent of S-SBRs. Compared to acetate, SAFL increased the proportion of large molecular weight organics and humic-like substances in effluent DOM by 74.87%–101.3% and 37.52%–48.35%, respectively, suggesting their bio-refractory nature. Molecular profiles analysis revealed that effluent DOM of S-SBRs exhibited a more diverse composition and a higher proportion of lignin-like molecules. Microorganisms-derived molecules were found to be the dominant fraction (71.51%–72.70%) in effluent DOM (<800 Da) of S-SBRs. Additionally, a prolonged hydraulic retention time enriched Bacteroidota, Haliangium and unclassified_f_Comamonadaceae , which benefited the degradation of DOM in S-SBRs. The results help to develop strategies on reducing effluent DOM in SAFL-fed BNR process. [Display omitted] • Characteristics of effluent DOM in SAFL-fed reactors were analyzed. • SAFL increased the proportion of high MW organics in effluent by 74.87%–101.3%. • Effluent DOM exhibited a more complex molecule profile in SAFL-fed reactors. • 71.51%–72.70% of the effluent DOM molecules in SAFL-fed reactors was SMP-derived. [ABSTRACT FROM AUTHOR]

In an anaerobic sequential batch reactor (SBR), marine anammox bacteria (MAB) were able to enhance microbial activity in nitrogen-rich saline wastewater and it was significantly affected by influent substrate composition and loading strength. This study therefore enhanced nitrogen removal efficiency by adjusting the influent nitrogen loading strength of MAB-inoculated anaerobic SBRs and assessed the correlation with the bacterial community. The results displayed that the system obtained optimal nitrogen removal efficiency (TN = 83.52%, NH 4 –N = 90.14%, and NO 2 –N = 83.57%) as the strength of influent nitrogen loading was increased to 201.35 mg L−1 for NH 4 –N and 266.42 mg L−1 for NO 2 –N. Moreover, the increase in the strength of influent nitrogen loading also enhanced the anammox 16S rRNA abundance (4.09 × 108 copies g−1) and ladderanes content (22.49 ng g−1 dw). Analysis of 15N isotope further illustrated that all systems were dominated by anammox (average ra = 95.22%). In conclusion, these findings provide scientific guidance for the management of eutrophic seawater and contribute to the realization of industrial applications for the treatment of nitrogen-rich saline wastewater. [Display omitted] • Increasing influent nitrogen loading strength enhances anammox nitrogen removal of saline wastewater. • Influent nitrogen load strength significantly affected the abundance of nitrogen transforming microorganisms. • Summed ladderanes increased with the increasing influent nitrogen loading strength. [ABSTRACT FROM AUTHOR]

Acid mine drainage (AMD) and municipal wastewater (MWW) are commonly co-occurring waste streams in mining regions. Co-treating AMD at existing wastewater facilities represents an innovative solution for simultaneous AMD reclamation and improved MWW treatment. However, unknowns related to biological processes and continuous treatment performance block full-scale use. The overarching goal of this work was to address questions related to efficacy and performance of continuous processing of AMD in a biological MWW treatment system. Synthetic AMD was co-treated with synthetic MWW in a continuously-operating bench-scale sequencing batch reactor (SBR). SBRs treated MWW with two strengths of AMD (91 and 720 mg/L as CaCO 3 Acidity) to capture the variations of coal AMD chemistry and strength observed in the field. Each co-treatment phases lasted 40+ days, during which clarified effluent and settled sludge quality was routinely monitored to determine impacts of co-treatment relative to conventional MWW treatment performance. Co-treatment produced effluent that met key standards for secondary treatment including biochemical oxygen demand (BOD) < 5 mg/L, total suspended solids (TSS) < 20 mg/L, and pH ∼7.0. Addition of AMD also improved treatment performance, increasing Phosphate (PO 4) removal by >60% and pathogen removal by an order of magnitude. Furthermore, AMD co-treatment did not exhibit any major impacts on the overall diversity of the wastewater microbial community. Co-treatment sludge had slightly higher settleability and a lower bound water content, but notable changes in sludge morphology was observed. This study demonstrates co-treatment allows for continuous mitigation of AMD without adversely impacting MWW treatment performance in conventional biological MWW processes. • Varying strength AMD continuously added to biological wastewater treatment process. • All treatment conditions met US Secondary Wastewater Standards (pH, BOD, and TSS). • Co-treatment generally did not impact overall wastewater microbial diversity. • PO 4 removal exceeded 90% during strongest AMD addition. • Settled secondary sludge contained less bound water and higher total solids. [ABSTRACT FROM AUTHOR]

To efficiently remove nutrients from low C/N sanitary sewage by conventional biological process is challenging due to the lack of sufficient electron donors. A novel electrolysis-integrated sequencing batch biofilm reactor (E-SBBR) was established to promote nitrogen and phosphorus removal for sanitary sewage with low C/N ratios (3.5–1.5). Highly efficient removal of nitrogen (>79%) and phosphorus (>97%) was achieved in the E-SBBR operating under alternating anoxic/electrolysis-anoxic/aerobic conditions. The coexistence of autotrophic nitrifiers, electron transfer-related bacteria, and heterotrophic and autohydrogenotrophic denitrifiers indicated synergistic nitrogen removal via multiple nitrogen-removing pathways. Electrolysis application induced microbial anoxic ammonia oxidation, autohydrogenotrophic denitrification and electrocoagulation processes. Deinococcus enriched on the electrodes were likely to mediate the electricity-driven ammonia oxidation which promoted ammonia removal. PICRUSt2 indicated that the relative abundances of key genes (hya A and hya B) associated with hydrogen oxidation significantly increased with the decreasing C/N ratios. The high autohydrogenotrophic denitrification rates during the electrolysis-anoxic period could compensate for the decreased heterotrophic rates resulting from insufficient carbon sources and nitrate removal was dramatically enhanced. Electrocoagulation with iron anode was responsible for phosphorus removal. This study provides insights into mechanisms by which electrochemically assisted biological systems enhance nutrient removal for low C/N sanitary sewage. [Display omitted] • E-SBBR showed enhanced capability for TN and TP removal for low C/N sanitary sewage. • Multiple nitrogen-removing pathways were involved in a single-stage E-SBBR. • Applied electrolysis enhanced ammonia removal via microbial anoxic ammonia oxidation. [ABSTRACT FROM AUTHOR]

The objective of the study was to explore the impact of different operation strategies on the performance of the Oxic-Settling-Anaerobic (OSA) process. A sequencing batch reactor was selected as the aerobic (oxic) unit of the OSA system and it was operated as a dual mixing phase with step feeding in order to optimize simultaneous nitrogen removal and sludge minimization. For this purpose, the effect of COD/N ratio, filling pattern and the fraction of anaerobic period was investigated on the performance of a laboratory-scale OSA system fed with synthetic wastewater (peptone mixture) approximating the characteristics of sewage. In nine consecutive experimental stages, each sustaining different metabolic processes and biochemical reactions, the sludge reduction potential of the OSA system was not impaired, maintaining an average observed yield value of 0.18 g VSS/g COD, which was approximately half the level observed in the reference reactor. Similarly, the OSA scheme of operation did not affect or alter the fundamental mechanisms of biological nitrogen removal, where system performance could be modified and optimized the same way as in a conventional activated sludge configuration. Furthermore, the OSA system maintained a higher level of biomass concentration compared with the reference reactor. This observation confirmed the previous analyses that the continuous biomass inflow from the side-stream anaerobic bioreactor was the reason to establish the microbial mechanism that resulted in a much lower sludge generation, regardless of the biochemical processes taking place in the reactor. Image 103724 • Simultaneous N removal and sludge minimization was optimized in an OSA system. • The main reactor was operated with a dual mixing phase and step feeding. • OSA reduced sludge production by almost 50% as compared with the control reactor. • Changing oxic reactor for nitrogen removal did not impair sludge reduction ratio. • Nitrogen removal efficiencies were increased up to 83% with higher COD/N ratios. [ABSTRACT FROM AUTHOR]

Ibuprofen (IBU) containing wastewater with a concentration of 1–5 mg/L was treated in an activated sludge sequencing batch reactor (SBR), for 60 days, in order to investigate the overall performance of the SBR, the parameter variations during a typical cycle, the chemical composition and content of extracellular polymeric substances (EPS) and the evolution of microbial community. The average removal efficiencies of COD, NH 4 +-N and TN were >85%, while >40% of the IBU was removed and the removal efficiencies of TP fluctuated around ~ 75%. The EPS content increased significantly with IBU addition (p < 0.01). Fulvic acid-like substances in the chemical composition of EPS increased during the stable operation phase. Proteobacteria associated with nitrogen removal was the dominant phylum, which can also resist IBU stress. For the denitrifying bacteria, the OTUs of both Rhodobacter and Pseudomonas increased from day 1–30 and reduced on day 60 (p < 0.01), which was opposite to the results observed for Rhodocyclaceae (phosphorus-accumulating bacteria). The OTUs of Acidovorax showed an increasing trend (p < 0.01), whereas the OTUs for Nitrospira (nitrite oxidizers) and Nitrosomonas (ammonia oxidizers) decreased significantly (p < 0.05). Image 1 • Good removal efficiency of COD, NH 4 +-N, TN, TP and ibuprofen was attained. • After adding ibuprofen, the content of EPS (PN and PS) highly significant increased (p < 0.01). • Read numbers of partial DNB and PAO highly significant increased (p < 0.01). • Proteobacteria associated with nitrogen removal was the dominant phylum. [ABSTRACT FROM AUTHOR]

This study investigated the influence of three different organic carbon sources including sodium acetate (SOD), glucose (GLU), and starch (STAR), on soluble microbial products (SMP), which presumably have dissimilar uptake rates and metabolic pathways, in sequencing batch reactors (SBR) and their subsequent effects on membrane fouling of ultrafiltration (UF). SMP were mainly characterized by fluorescence excitation emission matrix coupled with parallel factor analysis (EEM-PARAFAC) and size exclusion chromatography (SEC). SMP produced in SOD-fed SBR showed higher abundances of protein-like fluorescent component and large sized aliphatic biopolymer (BP) than GLU- or STAR-fed counterpart did, while the STAR-based operation resulted in more SMP enriched with humic-like fluorescence. The differences in SMP exerted marked effects on UF membrane fouling as indicated by the highest fouling potential with reversibility shown for the SMP from the SOD-fed reactor. Regardless of the carbon source, BP fraction and protein-like component exhibited the greatest extent of reversible fouling, suggesting that size exclusion plays a critical role. However, notable differences in the reversible fouling propensity of relatively smaller size fractions among the three SBRs signified the possible involvement of chemical interactions as a secondary fouling mechanism and its dependency on different carbon sources. Our results provide a new insight into the roles of carbon sources in the characteristics of SMP in biological treatment systems and their effects on the post-treatment using membrane filtration, which is ultimately beneficial to the optimization of biological treatment design and membrane filtration operation. Image 1 • Different organic carbon sources significantly alter SMP composition in SBRs. • Large sized biopolymers and protein-like component are more enriched in SOD-fed SBR. • SMP in SOD-fed SBR showed the highest reversible membrane fouling propensity. • Organic carbon source-dependent fouling is more pronounced for smaller sized SMP. [ABSTRACT FROM AUTHOR]

Anaerobic sludge granulation was evaluated in an expanded granular sludge bed (EGSB) reactor based on the increases in the specific organic loading rate (SOLR). The effect of precursor substances (calcium chloride, sodium chloride, and tannin) on the development of granular sludge was also investigated in batch reactors. The reactors were fed with synthetic sewage and operated in mesophilic conditions. The EGSB was operated with a variable hydraulic retention time (HRT) and the batch reactors, with cycles of 8 h and 16 h. The increase of SOLR from 17.4 ± 7.4 to 104.6 ± 66.7 mgCOD gVSS-1 d-1 in the EGSB resulted in an increase on the average granules diameter from 344.3 to 1583.3 μm. These conditions also favored the reduction rates of chemical oxygen demand (COD) and volatile fatty acids (VFAs) concentration in the reactor. When the upflow velocity suffered an abrupt increase (from 0.06 L h-1 to 0.25 L h-1), the granules size began to decrease and lose their settleability characteristics. Considering this, it is proposed to start the biomass granulation process without effluent recirculation, and, after the granules reach the desired size and settleability capacity, the normal operation of EGSB reactor starts. The results showed that calcium chloride was more efficient for granulation. CaCl 2 addition can be performed only during the reactor's start-up, improving granulation and reducing start-up time. Thus, these results have practical implications as granules maintenance is the key to the proper EGSB operation. • High upflow velocity decreases the granules' size in the start-up of the EGSB. • It is recommended to start the sludge granulation in UASB-type reactors. • The increase in the size of the granules favored COD removals. • Calcium chloride can be used as precursor agent for anaerobic sludge granulation. [ABSTRACT FROM AUTHOR]

Abstract Various water treatment processes may be ineffective to remove pharmaceutical compounds (PhCs) and their by-products, leading to endocrine-disruptive activity that might be detrimental to wildlife and human health. This study investigated the degradation of carbamazepine (CBZ), diclofenac (DCF), ibuprofen (IBP), and their intermediates, as well as estrogenic activity that is not effectively removed by conventional methods. A consortium of isolated South African indigenous fungi A. niger, M. circinelloides, T. polyzona, T. longibrachiatum and R. microsporus, was used in a sequencing batch reactor (SBR) to remove PhCs, their intermediates and strongly reduce their estrogenic activity. The fungal ligninolytic enzymatic activity was determined for laccase (Lac), manganese peroxidase (MnP) and lignin peroxidase (LiP) using a spectrophotometric method. The biodegradation of PhCs and their intermediates was monitored by SPE-UPLC/MS. The in vitro estrogenic activity was assessed in the T47D-KBluc reporter gene assay. Lac, MnP and LiP production appeared to be biomass growth dependent. During a lag phase of growth, a constant biomass of about 122.04 mg/100 mL was recorded with average enzymatic activity around 63.62 U/L for Lac, 151.91 U/L for MnP and 42.12 U/L for LiP. The exponential growth phase from day 7 to day 17, was characterised by a biomass increase of 124.46 units, and an increase in enzymatic activity of 9.91 units for Lac, 99.03 units for MnP and 44.24 units for LiP. These enzymes played an important synergistic role in PhCs degradation in the cytochrome P450 system. A decrease of 13.89%, 29.7% and 16.15% in PhC concentrations was observed for CBZ, DCF and IBP, respectively, and their intermediates were identified within 4 h of incubation. The removal efficiency achieved after 24 h in the SBR was about 89.77%, 95.8% and 91.41% for CBZ, DCF and IBP, respectively. The estradiol equivalent (EEq) values of 1.71 ± 0.30 ng/L and 2.69 ± 0.17 ng/L were recorded at the start-up time and after 4 h, respectively. The presence of intermediates was found to induce estrogenic activity. The EEq values after 24 h incubation was found to be below the LoQ and below the LoD of the assay. None of the samples exhibited any anti-estrogenic activity. The fungal consortium inoculum was found to induce toxicity at a 0.4× concentration, as observed under a microscope. This study revealed that the use of the fungal consortium can remove the estrogenic activity of pharmaceutical metabolites, which appeared to be the most significant contributors to the endocrine-disrupting activity of the wastewater treatment plant effluents. Graphical abstract Image 1 Highlights • A fungal consortium was successful for the bioremediation of synthetic wastewater. • Simultaneous degradation of carbamazepine, diclofenac and ibuprofen was observed. • Estrogenic activity was detected with metabolites compounds than parents. • Fungal consortium was found to remove the metabolites' estrogenic activity. [ABSTRACT FROM AUTHOR]

Abstract Greywater treatment and reuse can be considered a promising option, in particular in water scarcity affected areas. In this work a waste material, namely recycled corrugated wire hose cover, was applied as an alternative and cheap carrier in a sequencing batch biofilm reactor (SBBR) for greywater treatment. The bioreactor performance was studied in terms of organic matter, nitrogen and micropollutant removal. Four operational stages were investigated: i) inoculation of the carriers; ii) greywater treatment with suspended biomass; iii) synthetic and iv) real greywater treatment with inoculated carriers in the SBBR. The SBBR could treat real greywater showing high removal efficiencies for COD (86.5 ± 5.8%), ammonium (98.4 ± 1.4%) and total nitrogen (71.4 ± 8.2%). The obtained efficiencies were similar to the ones obtained with commercial carriers and to other treatments such as MBBR or MBR. In terms of micropollutants, 7 out of 13 detected micropollutants were highly removed (efficiency higher than 85%) while 5 of them (ofloxacin, metoprolol acid, venlafaxine, iopromide and hydrochlorothiazide) were found to be highly recalcitrant to the treatment. Graphical abstract Image 1 Highlights • Alternative low cost carriers were produced from recycled corrugated wire hose. • SBBR was effective removing 86.5% COD, 98.4% NH 4 + and 71.4% TN from greywater. • The performance of the alternative and commercial carriers was similar. • 7 Micropollutants were removed with an efficiency higher than 85%. • 5 micropollutants were found to be highly recalcitrant to the treatment. [ABSTRACT FROM AUTHOR]

Abstract The purpose of this research was the preparation and photocatalytic evaluation of a novel nanocomposite (NC) based on Fe 3 O 4 /ZnO, to eliminate four persistent antibiotics in surface waters: sulfamethoxazole, trimethoprim, erythromycin and roxithromycin. Prior to the operation of the photocatalytic reactor, the influence of pH (3–9), catalyst concentration (50–800 mg L−1), oxidant dose (0–100 mg L−1) and concentration of different targets (10–100 μg L−1) on the catalytic efficiency was evaluated. The analysis of reaction kinetics showed that degradation processes of the four antibiotics followed a pseudo-first-order kinetic model. Antibiotics adsorption onto the nanocomposite surface depended on their electrostatic nature and played an important role when decreasing the initial concentration of antibiotics. In this context, kinetic rates were higher at lower initial levels of organic pollutants, which is a favourable effect from a practical application perspective. On the other hand, a synergistic effect of the available Fe in the nanocomposite was found, contributing to the oxidation of antibiotics by photo-Fenton as a secondary reaction. Then, a magnetic photocatalytic reactor was operated under optimal conditions. The enhanced photonic efficiency of Fe 3 O 4 /ZnO in the system, as well as the ease of the magnetic separation and catalyst reusability, indicate the viability of this reactor configuration. Graphical abstract Image 1 Highlights • A nanocomposite (NC) of Fe 3 O 4 /ZnO was prepared and photocatalytically evaluated. • A magnetic photocatalytic reactor was operated to remove organic pollutants using NC. • Photocatalytic performance was consistent over 8 successive reaction cycles. • Toxicity levels in the samples were reduced after treatment. [ABSTRACT FROM AUTHOR]

Abstract As the efficiency of biological wastewater treatment in activated sludge systems can be increased if powdered mineral materials are added, the work described here investigated the effect of powdered keramsite on activated sludge and wastewater treatment in a sequencing batch reactor on the laboratory scale. Specifically, experiments were carried out on two sequencing batch reactors, with the reference system being the classical SBR with conventional activated sludge, while the second system involved a K-SBR (Keramsite - Sequencing Batch Reactor) with powdered keramsite added with a view to enhancing the effectiveness of the activated sludge. The concentration of powdered keramsite in the latter reactor was maintained at 0.75 g/L. The results confirmed the greater efficiency of biological wastewater treatment processes where activated sludge was supported by the keramsite. Highlights • Powdered keramsite improves floc sedimentation in activated-sludge system. • Use of the powdered keramsite stimulates the growth of microorganisms in activated sludge. • Powdered keramsite facilitates biological wastewater treatment processes. • Keramsite serves to stabilise biological wastewater treatment processes. [ABSTRACT FROM AUTHOR]

Abstract The occurrence of various antibiotics in natural waters poses an emerging environmental concern. Tetracycline (TC) is a frequently used antibiotic in human therapy, veterinary industry, and agricultural sectors. In the current study, TC removal from aqueous solutions was studied using binary Nickel/nano zero valent iron particles (NiFe nano particles) and in-situ NiFe nanoparticles coated sand (IS-NiFe). Removal of TC using bimetallic NiFe particles was optimized with help of response surface methodology (RSM). Using the optimized parameters (concentration of TC: 20 mg/L; NiFe dose: 120 mg/L; time of interaction: 90 min), 99.43 ± 0.98% removal of TC was noted. Further, IS-NiFe was packed in the column reactors and effects of different parameters like flow rate (1–3 mL/min), bed height (3–10 cm) and inlet TC concentration (20–60 mg/L) on breakthrough characteristics were examined. Under the optimized conditions the removal capacity in the column reactor was 1198 ± 40.2 mg/g using IS-NiFe. The column kinetic data were successfully fitted with Adams- Bohart and Thomas models. TC removal efficiency of IS-NiFe in column reactors was tested with TC (20 mg/L) spiked lake water, ground water, and tap water and the removal capacity was noted to be 698.55 ± 11.21, 764.17 ± 6.78, and 801.7 ± 13.26 mg/g respectively. Graphical abstract Image 1 Highlights • Ni/Fe and in-situ sand coated NiFe nanoparticles (IS-NiFe) synthesis. • NiFe nanoparticles in batch reactor removed 99.43 ± 0.98% of TC. • 1198 mg/g of TC removal capacity observed using IS-NiFe in column. • Sorption played important role in TC removal in continuous flow study. [ABSTRACT FROM AUTHOR]

A comprehensive investigation utilized a column-type sequencing batch reactor (SBR) to efficiently remove nutrients throughout various phases of its operational cycle by forming granules. This study assessed the influence and mechanisms of a simultaneous nitrification and denitrification (SND) system employing a column-type sequential batch reactor (SBR). The primary focus was on elucidating the functional groups involved in nitrogen transformation and removal within the extracellular polymeric substances (EPS). The research findings demonstrate the superior performance of the SBR process compared to the control group. It achieved an impressive SND efficiency of 69%, resulting in a remarkable 66% total nitrogen removal. Furthermore, a detailed analysis unveiled that the SBR process had a beneficial impact on the composition and properties of EPS. This impact was observed through increased EPS content and enhanced capacity to transport, convert, and retain nitrogen effectively. Additionally, after initial acclimatization, the SBR process showed its effectiveness in removing nutrients (88–98%) and COD (93%) from the generated wastewater within a hydraulic retention time (HRT) of 6 h. A statistically significant difference between the treatments for the investigated mixing ratios was found by univariate analysis of variance (ANOVA). Machine learning (CatBoost model) was employed to understand each parameter's relationship and predict the outcomes in measurable quantity. The findings of the SBR trials showed that the concentration of generated wastewater and the HRT impacted the treatment efficiency. However, the effluent may still need other physicochemical processes, such as membrane filtering, coagulation, electrocoagulation, etc., as post-treatment options, even though COD, nutrients, and turbidity have been entirely or significantly effectively removed. Overall, this work offers insightful information on the critical function of the SBR bacterial community in promoting SND. [Display omitted] • A sequencing batch reactor removed the nutrient using column-type sequencing batch reactors. • This study examined impact and mechanism for simultaneous nitrification and denitrification. • It achieved an SND efficiency of 69% and 66% of total nitrogen removal. • SBR removed nutrients (88–98%) and COD (93%) with hydraulic retention time (HRT) of 6 h. • Machine learning (CatBoost model) employed to understand the relationship of each parameter. [ABSTRACT FROM AUTHOR]

Sludge reduction is a major challenge in biological wastewater treatment. Hydrolytic enzymes secreted by thermophilic bacteria can lyse sludge and thus achieve sludge reduction, and the indigenous thermophilic community in sludge can lyse sludge more effectively. In this study, the feasibility of combining a sludge lysis reactor based on thermophilic bacteria community (LTBC reactor, 75 °C) with a conventional sequencing batch activated sludge reactor (SBR) for sludge reduction (i.e., LTBC-SBR process) was systematically investigated first time. The effect of lysed sludge returning to the biochemical tank on pollutant removal efficiency, sludge flocculation, sludge settling, and microbial community and function of the LTBC-SBR process was studied. In the LTBC 1 -SBR process, a sludge growth rate of 0.71 g TSS/day was observed when the lysed sludge reflux ratio (LRR) was 1, and the sludge generation was reduced by 81.5% compared to the conventional SBR reactor. In the LTBC 1 -SBR process, the removal efficiencies of chemical oxygen demand and total nitrogen were 94.0% and 80.5%, respectively. There was no significant difference in the sludge volume index from the SBR to the LTBC 1 -SBR stage, however, the effluent suspended solids concentration increased from 35.2 ± 2.1 mg/L to 80.1 ± 5.3 mg/L. This was attributed to the reflux of sludge lysate. In addition, the changes in extracellular polymers content and composition resulted in poor sludge flocculation performance. Heterotrophic bacteria associated with Actinobacteria and Patescibacteria enriched in LTBC 1 -SBR with relative abundance of 28.51 ± 1.25% and 20.01 ± 1.21%, respectively, which decomposed the macromolecules in the refluxed lysed sludge and contributed to the sludge reduction. Furthermore, due to the inhibition of nitrite-oxidizing bacteria, the nitrite concentration in the effluent of the LTBC 1 -SBR system reached 4.7 ± 1.1 mg/L, and part of the denitrification process was achieved by short-cut nitrification and simultaneous denitrification. These results indicate that in-situ sludge reduction technology based on lyse sludge lysing by thermophilic community has considerable potential to be widely used in wastewater treatment. [Display omitted] • LTBC-SBR process can reduce 81.5% excess sludge compared with SBR process. • COD and TN removal efficiencies in LTBC-SBR were 94.0% and 80.5%. • Enrichment of Actinobacteria and Patescibacteria promotes sludge reduction. • NOB Inhibition in LTBC-SBR promotes short-cut nitrification & denitrification. [ABSTRACT FROM AUTHOR]

Disinfection and decontamination of water by application of oxidisers is an essential treatment step across numerous industrial sectors including potable supply and industry waste management, however, could be greatly enhanced if operated as advanced oxidation processes (AOPs). AOPs destroy contaminants including pathogens by uniquely harnessing radical chemistry. Despite AOPs offer great practical opportunities, no reviews to date have highlighted the critical AOP virtues that facilitate AOPs' scale up under growing industrial demand. Hence, this review analyses the critical AOP parameters such as oxidant conversion efficiency, batch mode vs continuous-flow systems, location of radical production, radical delivery by advanced micro-/mesoporous structures and AOP process costs to assist the translation of progressing developments of AOPs into their large-scale applications. Additionally, the state of the art is analysed for various AOP inducing radical/oxidiser measurement techniques and their half-lives with a view to identify radicals/oxidisers that are suitable for in-situ production. It is concluded that radicals with short half-lives such as hydroxyl (10−4 μsec) and sulfate (30–40 μsec) need to be produced in-situ via continuous-flow reactors for their effective transport and dosing. Meanwhile, radicals/oxidisers with longer half-lives such as ozone (7–10 min), hydrogen peroxide (stable for several hours), and hypochlorous acid (10 min −17 h) need to be applied through batch reactor systems due to their relatively longer stability during transportation and dosing. Complex and costly synthesis as well as cytotoxicity of many micro-/mesoporous structures limit their use in scaling up AOPs, particularly to immobilising and delivering the short-lived hydroxyl and sulfate radicals to their point of applications. Overall, radical delivery using safe and advanced biocompatible micro-/mesoporous structures, radical conversion efficiency using advanced reactor design and portability of AOPs are priority areas of development for scaling up to industry. [Display omitted] • Radical conversion, location, delivery, operating mode and cost affect scalability • Radicals with short half-lives need to be produced in-situ via continuous mode • Radicals/oxidisers with longer half-lives need to be applied through batch systems • Conversion efficiency and portability of AOPs are priority areas of development [ABSTRACT FROM AUTHOR]

Treating wastewater using purple non-sulfur bacteria (PNSB) is an environmentally friendly technique that can simultaneously remove pollutants and lead to the accumulation of high-value cell inclusions. However, no PNSB system for treating heavy oil refinery wastewater (HORW) and recovering high-value cell inclusions has yet been developed. In this study, five batch PNSB systems dominated by Rhodopseudomonas were used to treat real HORW for 186 d. The effects of using different hydraulic retention times (HRT), sludge retention times (SRT), trace element solutions, phosphate loads, and influent loads were investigated, and the bacteriochlorophyll, carotenoid, and coenzyme Q 10 concentrations were determined. The community structure and quantity of Rhodopseudomonas in the systems were determined using a high-sequencing technique and quantitative polymerase chain reaction technique. The long-term results indicated that phosphate was the limiting factor for treating HORW in the PNSB reactor. The soluble chemical oxygen demand (SCOD) removal rates were 67.03% and 85.26% without and with phosphate added, respectively, and the NH 4 +-N removal rates were 32.18% and 89.22%, respectively. The NO 3 −-N concentration in the effluent was stable at 0–3 mg/L with or without phosphate added. Adding phosphate increased the Rhodopseudomonas relative abundance and number by 13.21% and 41.61%, respectively, to 57.35% and 8.52 × 106 gene copies/μL, respectively. The SRT was the limiting factor for SCOD removal, and the bacteria concentration was the limiting factor for nitrogen removal. Once the inflow load had been increased, the total nitrogen (TN) removal rate increased as the HRT increased. Maximum TN removal rates of 64.46%, 68.06%, 73.89%, 82.15%, and 89.73% were found at HRT of 7, 10, 13, 16, and 19 d, respectively. The highest bacteriochlorophyll, carotenoid, and coenzyme Q 10 concentrations were 2.92, 4.99, and 4.53 mg/L, respectively. This study provided a simple and efficient method for treating HORW and reutilizing resources, providing theoretical support and parameter guidance for the application of Rhodopseudomonas in treating HORW. • PNSB system with Rhodopseudomonas can treat heavy oil refinery wastewater effectively. • Phosphate improved SCOD and TN removal by 26.50% and 166.95% respectively in systems. • Phosphate obviously stimulated the growth of Rhodopseudomonas in PNSB system. • Rhodopseudomonas can adapt to higher concentrations of influent loads. • Compared to SCOD, nitrogen removal relied on longer HRT and higher bacterial concentration. [ABSTRACT FROM AUTHOR]

The need for environmental sustainability while increasing the quantity, quality, and the rate of waste treatment to generate high-value environmental friendly fertilizer products is highly in demand. Vermicomposting is a good technology for the valorisation of industrial, domestic, municipal and agricultural wastes. Various vermicomposting technologies have been in use from time past to present. These technologies range from windrow, small - scale batch vermicomposting to large – scale continuous flow systems. Each of these processes has its own merits and demerits, necessitating advancement in the technology for efficient treatment of wastes. This work explores the hypothesis that the use of a continuous flow vermireactor system of a composite frame structure performs better than batch, windrow and other continuous systems operated in a single container. Following an in-depth review of the literature on vermicomposting technologies, treatment techniques, and reactor materials used, to explore the hypothesis, it was found that vermireactors operating in continuous flow fashion perform better in waste bioconversion than the batch and windrow techniques. Overall, the study concludes that batch techniques using plastic vermireactors predominate over the other reactor systems. However, the use of frame compartmentalized composite vermireactors performs considerably better in waste valorisation. • Vermicomposting is far superior to traditional composting technique. • Continuous flow system vermireactors outperforms batch vermicomposting reactors and windrows in waste valorisation. • Materials used for vermireactor construction determines the reactors efficiency. • Frame compartmentalized composite vermireactors performs better than other vermireactors. • Precomposting exert significant influence on the waste stabilization time. [ABSTRACT FROM AUTHOR]

Abstract In order to intuitively and clearly evaluate the potential and current distribution, the fluid flow and mixing, as well as mass transfer involved in electrocoagulation process for As and Sb removal, numerical simulation of electric field, flow field and mass transfer were constructed by Comsol Multiphysics and verified by experiments. Results displayed that the primary current and potential distribution were improved by changing electrode distance or adding insulator in a batch reactor. When configuration 2 and 2 cm electrode distance were applied, a more uniform primary current distribution and higher electrode current efficiency were obtained. In a continuous flow reactor, the increase of flow rate resulted in the left shift of the peak in residence time distribution curve, gradual decrease of the tailing area, reduction of the stagnation zone, and more uniform mixing of the fluid. However, higher than 0.043 L/min was unfavorable to the formation of flocs and its effective combination with pollutants. According to the simulation of mass transfer, at the initial stage, the rate of electrolysis/hydrolysis was greater than that of mass transfer. Fe2+, OH−, and Fe(OH) 2 were primarily concentrated on the anode, cathode, and between the two electrodes, respectively. Under the action of electromigration, diffusion and convection, the concentration distribution of Fe(OH) 2 increased at the direction of streamline. The concentration of Fe2+ and OH− achieved the minimum value at the outlet. However, Fe(OH)+ concentration and distribution were hardly affected by the treatment time, and once generated, immediately proceed to the next hydrolysis reaction. Graphical abstract Image 1 Highlights • The primary current and potential distribution with different reactor configurations were modeled and improved. • The hydrodynamics of EC system were evaluated and the continuous flow reactor was optimized. • The diffusion, migration and convection of typical products were systematically analyzed. [ABSTRACT FROM AUTHOR]

The individual and mutual effects of substrate concentration (from 0.8 to 9.2 g/L) and pH (from 4.6 to 7.4) on hydrogen and volatile fatty acids production from sugarcane bagasse (SCB) were investigated in batch reactors, using a response surface methodology (RSM) and central composite design (CCD). The maximum of 23.10 mmoL H 2 /L was obtained under optimized conditions of 7.0 g SCB/L and pH 7.2, at 37 °C through the acetic acid pathway (1.57 g/L). Butyric and succinic acids were the major volatile fatty acids (VFA) produced in the fermentation process (from 0.66 to 1.88 g/L and from 1.06 to 1.65 g/L, respectively). According to the results, the RSM and CCD were useful tools to achieve high hydrogen production rates using Clostridium, Bacillus and Enterobacter, identified by Illumina sequencing (16S RNAr) in the fermentative consortium, and Clostridium and Paenibacillus, autochthonous bacteria from SCB. Significant changes were observed in the microbial community according to the changes in the independent variables, since the genera in the central point condition (5.0 g SCB/L and pH 6.0) were Lactobacillus , Escherichia and Clostridium, and Bacteroides and Enterobacter , which were identified in the optimized condition (7.0 g SCB/L and pH 7.2). [ABSTRACT FROM AUTHOR]

The performance, microbial enzymatic activity and microbial community of a sequencing batch reactor (SBR) have been explored under magnesium oxide nanoparticles (MgO NPs) stress. The NH 4 + -N removal efficiency kept relatively stable during the whole operational process. The MgO NPs at 30–60 mg/L slightly restrained the removal of chemical oxygen demand (COD), and the presence of MgO NPs also affected the denitrification and phosphorus removal. The specific oxygen uptake rate, nitrifying and denitrifying rates, phosphorus removal rate, and microbial enzymatic activities distinctly varied with the increase of MgO NPs concentration. The appearance of MgO NPs promoted more reactive oxygen species generation and lactate dehydrogenase leakage from activated sludge, suggesting that MgO NPs had obvious toxicity to activated sludge in the SBR. The protein and polysaccharide contents of extracellular polymeric substances from activated sludge increased with the increase of MgO NPs concentration. The microbial richness and diversity at different MgO NPs concentrations obviously varied at the phylum, class and genus levels due to the biological toxicity of MgO NPs. [ABSTRACT FROM AUTHOR]

In this study, a lab-scale anaerobic sequencing batch reactor (AnSBR) was used for the acidification of a pharmaceutical wastewater sourced from etodolac chemical synthesis tanks. The effects of the organic loading rate (OLR), and etodolac and sulfate concentrations on the acidification rate and microbial community in AnSBR were investigated at 35 °C with a hydraulic retention time (HRT) of 37 h, a pH of 5, and OLRs up to 5.2 kgCOD/m 3 ·day. The AnSBR accomplished a 60% acidification ratio and 50–60% etodolac removal at OLRs up to 2.6 kgCOD/m 3 ·day. However, at OLR = 3.9 kgCOD/m 3 ·day, acidification was not achieved due to sulfite inhibition; pre-ozonation was applied to overcome this sulfite inhibition. Although etodolac and COD removals were improved, the wastewater was not successfully acidified. Real-time polymerase chain reaction (Q-PCR) and fluorescent in situ hybridization (FISH) analyses revealed that acidification was inhibited by the dominance of sulfate reducing bacteria (SRB) over acidification bacteria in the AnSBR. However, increasing the OLR to 5.2 kgCOD/m 3 ·day led to toxicity stress in the SRB due to increased sulfite concentrations. Sulfate load fundamentally affected acidification process and microbial community composition. The presence of etodolac with concentration up to 56 mg/L did not have a significant effect on VFA production and the microbial community. [ABSTRACT FROM AUTHOR]

Petrochemical wastewater contains inhibitory compounds such as aromatics that are toxic to microorganisms during biological treatment. The compact and layered structure and the high amount of extracellular polymeric substances (EPS) in aerobic granular sludge (AGS) can contribute to protecting microorganisms from the harsh environment. This study evaluated the changes in the granule properties, pollutants removal, microbial metabolic potential and molecular microbial characteristics of the AGS process for petrochemical wastewater treatment. Granules treating petrochemical wastewater had a higher SVI 30 /SVI 5 value (0.94) than that treating synthetic wastewater. An increase in bioactivity and EPS secretion with higher bio-polymer composition, specifically the functional groups such as hydroxyl, alkoxy and amino in protein, was observed, which promoted biomass aggregation. The granules also had more than 2-fold higher specific oxygen utilization rate. The AGS-SBR process obtained an average COD removal of 93% during petrochemical wastewater treatment and an effluent bCOD of below 1 mg L−1. No obvious inhibition of nitrification and denitrification activity was observed in the processes attributed to the layered structure of AGS. The average effluent NH 4 +-N of 5.0 mg L−1 was obtained and TN removal efficiencies of over 80.0% was achieved. Molecular microbial analysis showed that abundant functional genera Stenotrophomonas and Pseudoxanthomonas contributed to the degradation of aromatics and other petroleum organic pollutants. They were enriched with the variation of group behavior while metabolisms of amino acids and carboxylic acids by the relevant functional genera (e.g., Cytophagia) were significantly inhibited. The enrichment of Flavobacterium and Thermomonas promoted nitrification and denitrification, respectively. This research revealed the rapid start-up, enhanced granule structural strength, high inhibition resistance and considerable performance of AGS-SBR for petrochemical wastewater treatment. [Display omitted] • AGS kept stability and high efficiency in petrochemical wastewater treatment. • Average COD removal of 93% and TN removal of over 80% were achieved. • High SVI 30 /SVI 5 and bioactivity with increased EPS secretion were observed. • Stenotrophomonas and Pseudoxanthomonas contributed to petroleum organic degradation. • AGS process showed significant potential for petrochemical wastewater treatment. [ABSTRACT FROM AUTHOR]

Simultaneous bio-treatment processes of organic carbon (C)-, nitrogen (N)-, and phosphorus (P)-containing wastewater are challenged by insufficient carbon sources in the effluent. In the present study, two parallel anaerobic/aerobic sequencing batch reactors (R-1 and R-2) treating low C/N (≤4) wastewater were employed using different partial nitrification start-up strategies, controlled reduced aeration, and decreased sludge retention time. Advanced removal efficiencies for NH 4 +-N (≥96%), total nitrogen (TN, ≥86%), PO 4 3−–P (≥95%), and COD intra (≥91%) were realized, with TN and PO 4 3−–P effluent concentrations of 10.0 ± 3.5 and 0.11 ± 0.3 mg/L in R-1 and 9.28 ± 4.0 and 0.11 ± 0.1 mg/L in R-2, respectively. Higher nitrite accumulation rate (nearly 100%) and TN (121.1 ± 0.7 mg TN/g VSS·d) and P (12.5 ± 0.6 mg PO 4 3−–P/g VSS·d) removal loadings were obtained in R-2 by a thorough elimination of nitrite-oxidizing bacteria. Moreover, different microbial structures and nutrient removal pathways were identified. Denitrifying glycogen-accumulating organisms (Candidatus Competibacter) and phosphorus-accumulating organisms (PAOs) (Tetrasphaera) removed N and P with partial nitrification–endogenous denitrification pathways and aerobic P removal in R-1. In R-2, aerobic denitrifying bacteria (Psychrobacter) and PAOs ensured N and P removal through the partial nitrification–aerobic denitrification and aerobic P removal pathways. Compared to R-1, R-2 offers greater efficiency, convenience, and scope to further reduce carbon-source demand. [Display omitted] • Two novel start-up strategies of partial nitrification were developed. • COD intra efficiency reached to 93.3% in R-1. • Aerobic PAOs Tetrasphaera dominated in both systems. • The high-efficient use of oxygen by PAOs in the aerobic stage help AOB and DGAOs. • Aerobic denitrification in R-2 by Psychrobacter. [ABSTRACT FROM AUTHOR]

This study was to develop biogranules using a sequencing batch reactor (SBR) and to evaluate the effect of pineapple wastewater (PW) as a co-substrate for treating real textile wastewater (RTW). The biogranular system cycle was 24 h (2 stages of phase), with an anaerobic phase (17.8 h) followed by an aerobic phase (5.8 h) for every stage of the phase. The concentration of pineapple wastewater was the main factor studied in influencing COD and color removal efficiency. Pineapple wastewater with different concentrations (7, 5, 4, 3, and 0% v/v) makes a total volume of 3 L and causes the OLRs to vary from 2.90 to 0.23 kg COD/m3day. The system achieved 55% of average color removal and 88% of average COD removal at 7%v/v PW concentration during treatment. With the addition of PW, the removal increased significantly. The experiment on the treatment of RTW without any added nutrients proved the importance of co-substrate in dye degradation. • Biogranulation technology for the treatment of RTW, highly alkaline non-biodegradable textile wastewater under the influence of PW as a co-substrate. • Color and COD removal increased with the increasing volume of PW (%v/v). • Glucose and fructose in the PW could have contributed to the decolorization process as an electron donor. [ABSTRACT FROM AUTHOR]

The aim of this work was to study the effect of organic loading rate on the production of Polyhydroxyalkanoates (PHA) from sewage sludge. Synthesis of PHA using sewage sludge as platform was achieved in this work. Three pilot-scale selection-sequencing batch reactors (S-SBR) were used for obtaining a culture able to accumulate PHA following a strategy of aerobic dynamic feeding (ADF) at different volumetric organic-loading-rate (vOLR): 1.3, 1.8 and 0.8 g COD L−1 d−1 for S-SBR 1, S-SBR 2 and S-SBR 3, respectively. Decreasing the vOLR enhanced the general performance of the process as for organic matter removal (from 99.2% ± 0.3% in S-SBR-3 to 92 ± 2 in S-SBR-2) while the opposite trend was recorded for PHA production (6.0 PHA % w/w in S-SBR-3 vs 13.7 PHA % w/w in S-SBR-2 at the end of the feast phase). Furthermore, indirect and direct emissions, as N2O, were evaluated during the process for the first time. Finally, three accumulation tests were performed achieving 24% w/w. [Display omitted] • Effect of different OLR in MMC selection was studied. • OLR influenced F/F and PHA storage yield. • Low OLR enhance reactor's performance. • Higher OLR enhanced PHA production (13% w/w at the end of the feast). • Indirect GHG were higher than direct. [ABSTRACT FROM AUTHOR]

The performance, microbial enzymatic activity and microbial community of a sequencing batch reactor (SBR) were explored under instantaneous Cd(II) shock loading. After a 24-h Cd(II) shock loading of 100 mg/L, the chemical oxygen demand and NH 4 +-N removal efficiencies decreased significantly from 92.73% and 99.56% on day 22 to 32.73% and 43% on day 24, respectively, and then recovered to the normal values gradually. The specific oxygen utilization rate (SOUR), specific ammonia oxidation rate (SAOR), specific nitrite oxidation rate (SNOR), specific nitrite reduction rate (SNIRR) and specific nitrate reduction rate (SNRR) decreased by 64.81%, 73.28%, 77.77%, 56.84% and 52.46% on day 23 in comparison with the absence of Cd(II) shock loading, respectively, and they gradually returned to the normal levels. The changing trends of their associated microbial enzymatic activities including dehydrogenase, ammonia monooxygenase, nitrite oxidoreductase, nitrite reductase and nitrate reductase were in accordance with SOUR, SAOR, SNOR, SNIRR and SNRR, respectively. Cd(II) shock loading promoted the microbial reactive oxygen species production and lactate dehydrogenase release, indicating that instantaneous shock caused oxidative stress and damaged to cell membranes of the activated sludge. The microbial richness and diversity, and the relative abundance of Nitrosomonas and Thauera obviously decreased under the stress of Cd(II) shock loading. PICRUSt prediction showed that Cd (II) shock loading significantly affected Amino acid biosynthesis, Nucleoside and nucleotide biosynthesis. The present results are conducive to take adequate precautions to reduce the adverse effect on bioreactor performance in wastewater treatment systems. [Display omitted] • The COD and NH 4 +-N removal sharply decreased under the Cd(II) shock loading. • The Cd(II) shock inhibited nitrogen removal rates and microbial enzymatic activities. • The Cd(II) shock induced the ROS production and LDH release of activated sludge. • The Cd(II) shock reduced the microbial richness and diversity obviously. • The SBR performance gradually recovered after the instantaneous Cd(II) shock. [ABSTRACT FROM AUTHOR]

The formation of dominant phosphate-accumulating organisms (PAOs) is essential for the high enrichment of phosphate in biofilm sequencing batch reactors (BSBR) for phosphorus recovery. The dominant PAOs in the biofilm process have not been isolated and purified, and the key metabolic pathways that promote the formation of dominant PAOs are still unclear. In this study, four strains of highly-efficient PAOs were obtained by an innovative isolation procedure. The relationship between the abundance of highly-efficient and dominant PAOs and the phosphate removal ability was compared. We found that the abundance of PAOs was positively correlated with the phosphate removal efficiency in vitro pure culture and complex biofilm process. Metagenomics analysis revealed that compared with highly-efficient PAOs cultured in vitro , dominant PAOs in biofilms had unique key metabolic pathways, F-ATPases and N-Acyl homoserine lactones (AHLs). F-ATPases are important for maintaining the proton motive force (PMF) required for the uptake of carbon sources by PAOs, and AHLs are participating in phosphate metabolism through quorum sensing (QS) mediated secretion of extracellular polymeric substance (EPS). The formation of dominant PAOs was promoted by optimizing carbon source uptake and phosphate metabolism. This study revealed that the difficult isolation of dominant PAOs was due to the AHLs-mediated QS, and we identified the key pathways regulating the formation of dominant PAOs in biofilms through genomics analysis. Our findings provide insights in enhancing phosphate enrichment in BSBR by modulating the components of microbial community under the low concentration of carbon source consumption. [Display omitted] • The abundance of PAOs was positively correlated with phosphate removal efficiency. • Difficulty in isolation and screening of dominant PAOs is closely related to AHLs. • The key pathways regulating the formation of dominant PAOs are AHLs and F-ATPase. • Nutrition stress, oxidation and stress condition can stimulate the secretion of AHLs. • Balanced regulation of the carbon source and pH promotes the activation of F-ATPase. [ABSTRACT FROM AUTHOR]

Biogranulation technology is an emerging biological process in treating various wastewater. However, the development of biogranules requires an extended period of time when treating wastewaters with high oil and grease (O&G) content. A study was therefore conducted to assess the formation of biogranules through bioaugmentation with the Serratia marcescens SA30 strain, in treating real anaerobically digested palm oil mill effluent (AD-POME), with O&G of about 4600 mg/L. The biogranules were developed in a lab-scale sequencing batch reactor (SBR) system under alternating anaerobic and aerobic conditions. The experimental data were assessed using the modified mass transfer factor (MMTF) models to understand the mechanisms of biosorption of O&G on the biogranules. The system was run with variable organic loading rates (OLR) of 0.69–9.90 kg/m3d and superficial air velocity (SAV) of 2 cm/s. After 60 days of being bioaugmented with the Serratia marcescens SA30 strain, the flocculent biomass transformed into biogranules with excellent settleability with improved treatment efficiency. The biogranules showed a compact structure and good settling ability with an average diameter of about 2 mm, a sludge volume index at 5 min (SVI 5) of 43 mL/g, and a settling velocity (SV) of 81 m/h after 256 days of operation. The average removal efficiencies of O&G increased from 6 to 99.92%, respectively. The application of the MMTF model verified that the resistance to O&G biosorption is controlled via film mass transfer. This research indicates successful bioaugmentation of biogranules using the Serratia marcescens SA30 strain for enhanced biodegradation of O&G and is capable to treat real AD-POME. • Biogranulation technology is suitable for treating oily wastewater. • Bioaugmentation with the addition of Serratia marcescens SA30 enhanced the development of biogranules. • After the addition of Serratia marcescens SA30, the degradation of O&G increased. • Application of the MMTF model verified that mass transfer resistance is dependent on the [ k L a ] f. [ABSTRACT FROM AUTHOR]

Two novel and eco-friendly redox mediators (RMs), magnetic oxidative vanillin (MOV) and magnetic oxidative syringaldehyde (MOS), both derived from lignin, were prepared to improve the decolorization of the methyl orange (MO) dye. The Decolorization Efficiency (DE) of MO in the batch experiments with MOV and MOS were increased by more than 60% and 22%, respectively, when compared to the control experiment without magnetic RMs. Moreover, the two magnetic RMs could maintain stable DE of MO in sequenced batch reactors (SBRs), and negligible leaching of the oxidized lignin monomers was observed under various environmental conditions. Density Function Theory (DFT) calculations were used to propose three potential biodegradation mechanisms for azo dyes, and the key intermediates were confirmed using high-performance liquid chromatography. This study proposed a feasible strategy for functional utilization of lignin resource, as well as a practical method for effectively treating azo dye-containing wastewater. [Display omitted] • A kind of novel and eco-friendly redox mediators (RMs) derived from lignin were prepared. • The induction of RMs accelerated the decolorization of methyl orange (MO). • Magnetic immobilized in sequenced batch reactors, RMs maintained stable removal of MO. • Three stimulative mechanisms of the RMs for azo dyes were proposed. [ABSTRACT FROM AUTHOR]

The textile industry demands huge volumes of high quality water which converts into wastewater contaminated by wide spectrum of chemicals. Estimation of textile wastewater influence on the aquatic systems is a very important issue. Therefore, closing of the water cycle within the factories is a promising method of decreasing its environmental impact as well as operational costs. Taking both reasons into account, the aim of this work was to establish the acute toxicity of the textile wastewater before and after separate chemical, biological as well as combined chemical-biological treatment. For the first time the effects of three different combinations of chemical and biological methods were investigated. The acute toxicity analysis were evaluated using the Microtox ® toxicity test. Ozonation in two reactors of working volume 1 dm 3 (stirred cell) and 20 dm 3 (bubble column) were tested as chemical process, while biodegradation was conducted in two, different systems – Sequence Batch Reactors (SBR; working volume 1.5 dm 3 ) and Horizontal Continuous Flow Bioreactor (HCFB; working volume 12 dm 3 ). The untreated wastewater had the highest toxicity (EC50 value in range: 3–6%). Ozonation caused lower reduction of the toxicity than biodegradation. In the system with SBR the best results were obtained for the biodegradation followed by the ozonation and additional biodegradation – 96% of the toxicity removal. In the second system (with HCFB) two-stage treatment (biodegradation followed by the ozonation) led to the highest toxicity reduction (98%). [ABSTRACT FROM AUTHOR]

In the present study, a novel green bio-sorption reactor (GBR) was firstly proposed and preliminarily investigated by embedding constructed wetland (CW) into the aeration tank of the conventional activated sludge (CAS). This integrated novel system owns the striking features of adding carriers of wetland substrate (i.e. the dewatered alum sludge in this case) in CAS for robust phosphorus adsorption and enriching the biomass. Meanwhile, the “green” feature of this GBR imparted aesthetic value of CW to the CAS system. The preliminary 3-month trial of GBR based on a sequencing batch reactor (GB-SBR) with diluted piggery wastewater demonstrated an average removal of 96%, 99% and 90% for BOD, TP and TN, respectively. The comparison with moving bed biofilm reactor (MBBR) and integrated fixed-film activated sludge (IFAS) reflected the advantages of GBR over purification performance, aesthetic value and potential carbon sink. Moreover, the carriers used in the GBR are dewatered alum sludge which is in line with the policy of “recycle, reuse and reduce”. Overall, this GBR undoubtedly offered a more sustainable and economical solution for retrofitting the aging CAS. [ABSTRACT FROM AUTHOR]

The study aims to understand the occurrence and removal of 20 emerging contaminants (ECs) in each unit process of a sequencing batch reactor-based wastewater treatment plant (WWTP) and explore the potential of biological activated carbon (BAC) for the treatment of residual ECs and organic matter in the secondary effluent. Analgesic-acetaminophen, anti-inflammatory drug-ibuprofen, and stimulant-caffeine were detected at high concentrations in the influent. Most of the removal was observed in the biological treatment stage in the SBR basins. The mass load of the ECs was 2.93 g/d in the secondary effluent and 0.4 g/d in the final sludge, while the total removal of the mass load of ECs till the secondary treatment stage was 93.22%. 12 of the 20 ECs were removed by more than 50%, while carbamazepine (negative removal), sulfamethoxazole, and trimethoprim were removed by less than 20%. As a polishing step and to remove residual ECs, two BAC units were studied for 11,000 bed volumes (324 days). Packed column studies on granular activated carbon were conducted, and GAC development to BAC was monitored. SEM and FTIR were used to confirm and characterize the BAC. The BAC appeared to be more hydrophobic than the GAC. The BAC removed 78.4% and 40% of the dissolved ECs and organic carbon at an optimum EBCT of 25 min. Carbamazepine, sulfamethoxazole, and trimethoprim were removed by 61.5, 84, and 52.2%, respectively. Parallel column tests revealed adsorption as an important mechanism for the removal of positively charged compounds. The results indicate that the BAC is an effective tertiary/polishing technique for removing organic and micropollutants in the secondary wastewater effluent. [Display omitted] • 8 out of the 20 ECs were removed by less than 50% in the SBR-based treatment plant. • The GAC tends to get lipophilic while transitioning to BAC. • Adsorption is an important pathway in removing positively charged ECs in BAC. • BAC removed 78.4% of ECs and 40% of the organic carbon. [ABSTRACT FROM AUTHOR]

A novel coupling process (SAPD-A) with sulfide autotrophic partial denitrification (SAPD) (NO 3 −-N→NO 2 −-N) and anaerobic ammonium oxidation (Anammox) was developed using anaerobic sequencing batch reactor (ASBR) in this work. The integrated process comprised two stages. Firstly, the starting-up of SAPD process succeeded by gradually increasing the influent nitrate and sulfide in 95 days. The average nitrate removal efficiency (NRE) and NO 2 −-N accumulation rates were 71.24% ± 0.21% and 46.44% ± 0.53% at SAPD process (days 75–95). Then, successful coupling process (SAPD-A) was implemented in two stages (stage I and stage II of SAPD-A). In stage I, it is feasible to promote the successful construction of SAPD-A process by elevating influent ammonium only based on SAPD system, making the NRE increased from 44.45% ± 0.46% (day 95) to 64.62% ± 0.12% at the end of stage I in SAPD-A system (day 126). Meanwhile, the ammonium nitrogen removal efficiency (ARE) and total nitrogen removal efficiency (TN-RE) also rose up to 42.46% ± 2.02% and 63.28% ± 0.54% respectively. Furthermore, the average ARE, NRE and TN-RE during the stage II in the bioreactor could reach 65.17% ± 1.45%, 74.50% ± 0.81% and 77.81% ± 0.37% by loading some biofilters (with of approximate 10% of the volume of the bioreactor) attached anaerobic ammonium oxidation bacteria (AnAOB). High-throughput sequencing results showed that the dominant genera concerning nitrogen removal were norank_f_norank_o_Fimbriimonadates (with the abundance of 2.88–8.54%), norank_ o_ norank _ c_ OM190 (2.48–4.41%), norank_f_norank_o_norank_c_WWE3 (11.01–17.69%), subgroup_10 (1.97–3.81%), Limnobacter （2.17–3.49%）, norank_f_n orank_ o_norank_ c_OLB14 (2.03–5.23%), norank-f-PHOS-HE36 (2.18–5.5%), Ellin6067 (1.34–2.24%) and Candidatus_ Brocadia (1.95–2.42%) during the whole starting-up period of coupling SAPD-A process. Batch experiments revealed that the sulfide was fully oxidized within 2 h, with the maximum reaction rate of 38.30 ± 1.53 mg (L h)−1 in the first 1 h. Simultaneously, the concentration of nitrate sharply decreased from 53.08 ± 0.23 mg L−1 to 24.16 ± 0.42 mg L−1 with the reaction rate of 66.41 ± 2.12 mg (L h)−1 in 0.5 h. Also, the ammonium concentration significantly declined from 47.88 ± 0.34 mg L−1 to 10.98 ± 0.39 mg L−1 in 8 h. Anammox process was responsible for the dominant nitrogen removal in the coupling SAPD-A system. [Display omitted] • SAPD-A process was developed to treat nitrate and ammonium contained wastewater. • Contributions of nitrogen removal and microbial structures were investigated. • High TN removal efficiency could rise to 77.81% in the SAPD-A bioreactor. • Sulfur and nitrogen conversion mechanism in the SAPD-A system was proposed. [ABSTRACT FROM AUTHOR]

Pyrite-based autotrophic denitrification (PAD) is an emerging biological process to diminish nitrate pollution, but the relatively low NO 3 −-N removal rate limits its practical application. In this research, a pyrite-intensified hybrid sequencing batch biofilm reactor (PIHSBBR) was designed to treat low C/N ratio domestic wastewater. The results showed that PIHSBBR could achieve optimal removal of COD, NH 4 +-N, and TN under the aeration rate of 1.0 L/L∙min and the hydraulic retention time (HRT) of 8 h, with removal rates of 69.67 ± 4.37%, 77.04 ± 4.84%, and 63.92 ± 6.66%, respectively. The PAD efficiency in PIHSBBR during the stable operation was not high (13.05–31.01%), and the main nitrogen removal pathway in PIHSBBR, especially in the aerobic zone, was simultaneous nitrification and denitrification (SND). High-throughput sequencing analysis unraveled that Planctomycetota (3.65%) had a high abundance in the anoxic zone of PIHSBBR, implying that anaerobic ammonium oxidation (anammox) might have occurred in the anoxic zone. In addition, the nitrogen cycle function gene with the highest abundance was nirBD , indicating the possible presence of dissimilatory nitrate reduction to ammonium (DNRA) within the system (aerobic and anoxic zones). Our research can provide useful information for the improvement and future application of PIHSBBR. [Display omitted] • Mixotrophic denitrification was achieved in the pyrite-intensified hybrid SBBR. • The optimal working conditions were at aeration rate = 1.0 L/L∙min and HRT = 8 h. • Anaerobic ammonium oxidation (anammox) occurred in the anoxic zone. • The nitrogen removal pathway in the upper aerobic zone of PIHSBBR was SND. • The conversion pathway of nitrogen and sulfur in PIHSBBR was revealed. [ABSTRACT FROM AUTHOR]

pH is one of the major parameters that influence the granulation and long-term operation of aerobic granular sludge (AGS). In hypersaline wastewater, the impact of pH on granulation and the extent of organic loading rate (OLR) that AGS can withstand under different pH are still not clear. In this study, AGS was cultivated at 3% salinity in three sequencing batch reactors with influent pH values of 5.0, 7.0, and 9.0, respectively, and the OLR was stepwise increased from 2.4 to 16.8 kg COD/m3·d after the granules maturation. The results showed the satisfactory granulation and organic removal under different influent pH conditions, in which the granulation was completed on day 43, 23, and 23, respectively. Neutral influent was the most appropriate for development of salt-tolerant aerobic granular sludge (SAGS), while acidic environment induced the formation of fluffy filamentous granules, and alkaline environment weakened the granule stability. Metagenomic analysis revealed the similar microbial community of neutral and alkaline conditions, with the predominance of genus Paracoccus_f__Rhodobacteraceae. While in acidic environment, fungus Fusarium formed the skeleton of filamentous granules and functioned as the carrier of bacteria including Azoarcus and Pararhodobacter. With the elevation of OLR, SAGSs were found to maintain the compact structure under OLRs of 2.4, 7.2, and 2.4 kg COD/m3·d, and obtain high TOC removal (>95.0%) under OLRs of 7.2, 14.4, and 14.4 kg COD/m3·d, respectively. For hypersaline high-strength organic wastewater, satisfactory TOC removal could also be obtained at broad pH ranges (5.0–9.0), in which neutral environment was the most suitable and acidic environment was the worst. This study contributed to a better understanding of SAGS granulation and treatment of hypersaline high-strength organic wastewater with different pH values. [Display omitted] • Granulation and high-strength wastewater treatment at varied pH were first studied. • AGSs successfully formed in hypersaline wastewater with influent pH of 5, 7, and 9. • Longer time was needed to form fluffy filamentous granules with influent pH of 5. • SAGSs maintained compact under 2.4 (pH 5), 7.2 (pH 7), and 2.4 kg COD/m3·d (pH 9). • TOC removal was >95% under 7.2 (pH 5), 14.4 (pH 7), and 14.4 kg COD/m3·d (pH 9). [ABSTRACT FROM AUTHOR]

The fate and presence of nanoplastics in wastewater treatment systems is a topic of increasing interest. Furthermore, challenges related to their quantification and identification have made it difficult to set up experimental conditions and compare results between studies. In this study, the effect of 100 nm polystyrene nanoplastics on activated sludge was evaluated. A concentration of 2 μg/L was used to continuously feed a sequencing batch reactor (SBR-NPs). Under the experimental conditions used in this study, no changes were observed in the process performance of the SBR-NPs compared to the reactor used as a control. Neither nitrification nor organic matter removal efficiency, which was 96% for both SBRs, were affected by the presence of 100 nm polystyrene nanoplastics, which suggests that the tested nanoplastics were not sufficiently toxic to the biomass. Although no significant differences in the relative abundances of predominant phyla between SBR-Control and SBR-NPs were observed, a slight shift in the relative abundance of Patescibacteria (1.5 ± 0.6% and 3.7 ± 0.8% in SBR-Control and SBR-NPs, respectively, at the end of the test) occurred. The higher abundance of this phylum in SBR-NPs compared to SBR-Control may suggest that these bacteria have some sensitivity to the presence of 100 nm polystyrene nanoplastics. Furthermore, even with the absence of nitrification inhibition, it was observed stagnation of the growth of Nitrotoga bacteria in SBR-NPs, which also suggests that the polystyrene nanoplastics could have an inhibitory effect on these cells and an impact on nitrification in the long term. • Organic matter removal was not affected by 100 nm polystyrene nanoplastics. • Differences between the microbial populations in SBRs (control and with NPs) were found. • Nitrotoga bacteria abundance was reduced in SBR with 100 nm polystyrene nanoplastics. • Nanoplastics enhanced the abundance of Patescibacteria in the activated sludge. • Moderate heteroaggregation of nanoplastics was found in the activated sludge. [ABSTRACT FROM AUTHOR]

Partial nitrification is an effective process for treating high-strength ammonium landfill leachate with low C/N ratio, for the cooperation with denitrification can save almost 40% carbon addition in biological nitrogen removal. However, high ammonia loading often causes the instability of partial nitrification process. Less carbon addition can promote the stability of partial nitrification and increase the nitrite accumulation ratio (NAR). Nevertheless, the microbial mechanisms within remain further elusive. In this study, two laboratory-scale sequencing batch reactors were constructed and operated for 125 days, which were fed with ammonia synthetic wastewater with C/N of 0.6 (CN system) and C/N of 0.0 as the control (N system). CN system performed more stably and had the highest NAR of 100%. Extracellular polymeric substances (EPS) generated from carbon source provided spatial and nutrient niches to tighten the cooperation of functional microorganisms, thus, enhanced the stability and efficiency of partial nitrification. Thauera was the dominant denitrifier in CN system. Nitrosomonas was one of the most important autotrophic ammonia oxidizing bacteria, while Paracoccus and Flavobacterium were the main heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria in CN system. The enrichment of HN-AD bacteria outcompeted nitrite oxidizing bacteria (NOB), therefore leaded to higher nitrite accumulation in CN system. The findings of this study may be conducive to increasing the understanding of the microbial collaboration mechanisms of partial nitrification, thereby provides theoretical support for the improvement of biological nitrogen removal technology. [Display omitted] • CN system performs more stably in high ammonia loading phase. • Carbon addition promotes the partial nitrification in CN system. • EPS provides spatial and nutrient niches to tighten functional microorganisms. • Cooperation of multiple functional bacteria in CN system promotes the stability. • Diverse functional bacteria outcompete nitrite oxidizing bacteria in CN system. [ABSTRACT FROM AUTHOR]

Contamination of water by phenol is potentially a serious problem due to its high toxicity and its acid character. In this way some treatment process to remove or reduce the phenol concentration before contaminated water disposal on the environment is required. Currently, phenol can be removed by charcoal adsorption, but this process does not allow easy regeneration of the adsorbent. In contrast, polymeric resins are easily regenerated and can be reused in others cycles of adsorption process. In this work, the interaction of phenol with two polymeric resins was investigated, one of them containing a weakly basic anionic exchange group (GD-DEA) and the other, a strongly basic group (GD-QUAT). Both ion exchange resins were obtained through chemical modifications from a base porous resin composed of glycidyl methacrylate (GMA) and divinyl benzene (DVB). Evaluation tests with resins were carried out with 30 mg/L of phenol in water solution, at pH 6 and 10, employing two distinct processes: (i) batch, to evaluate the effect of temperature, and (ii) continuous flow, to assess the breakthrough of the resins. Batch tests revealed that the systems did not follow the model proposed by Langmuir due to the negative values obtained for the constant b and for the maximum adsorption capacity, Q 0 . However, satisfactory results for the constants K F and n allowed assuming that the behavior of systems followed the Freundlich model, leading to the conclusion that resin GD-DEA had the best interaction with the phenol when in a solution having pH 10 (phenoxide ions). The continuous flow tests corroborated this conclusion since the performance of GD-DEA in removing phenol was also best at pH 10, indicating that the greater availability of the electron pair in the resin with the weakly basic donor group contributed to enhance the resin's interaction with the phenoxide ions. [ABSTRACT FROM AUTHOR]

The effects of chlortetracycline (CTC) on the performance, microbial activity, extracellular polymeric substances (EPS) and microbial community of a sequencing batch biofilm reactor (SBBR) were investigated in treating mariculture wastewater. Low CTC concentration (less than 6 mg/L) had no obvious effect on the SBBR performance, whereas high CTC concentration could inhibit the chemical oxygen demand (COD) and nitrogen removal of the SBBR. The microbial activity of the biofilm in the SBBR decreased with the increase of CTC concentration from 0 to 35 mg/L. The protein (PN) contents were always higher than the PS contents in both loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) at different CTC concentrations. The chemical compositions of LB-EPS and TB-EPS had obvious variations with the increase of CTC concentration from 0 to 35 mg/L. The high-throughput sequencing revealed the effects of CTC on the microbial communities of the biofilm at phylum, class and genus level. The relative abundances of some genera displayed a decreasing tendency with the increase of CTC concentration from 0 to 35 mg/L, such as Nitrospira , Paracoccus , Hyphomicrobium , Azospirillum . However, the relative abundances of the genera Flavobacterium , Aequorivita , Buchnera , Azonexus and Thioalbus increased with the increase of CTC concentration. [ABSTRACT FROM AUTHOR]