1. In vitroandin vivogenotoxicity assessment of selected pharmaceuticals in relation toEscherichia coliandCyprinus carpio
- Author
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Monika Załęska-Radziwiłł, Andrzej Affek, Nina Doskocz, and Katarzyna Affek
- Subjects
Carps ,Environmental Engineering ,0211 other engineering and technologies ,02 engineering and technology ,In Vitro Techniques ,010501 environmental sciences ,Biology ,medicine.disease_cause ,01 natural sciences ,Cyprinus ,Toxicology ,Ciprofloxacin ,In vivo ,Escherichia coli ,medicine ,Animals ,Humans ,Carp ,0105 earth and related environmental sciences ,021110 strategic, defence & security studies ,Estradiol ,Mutagenicity Tests ,General Medicine ,biology.organism_classification ,Molecular biology ,Comet assay ,SOS chromotest ,S9 fraction ,Comet Assay ,Fluorouracil ,Genotoxicity ,DNA Damage ,Mutagens - Abstract
Genotoxicity studies (using SOS chromotest and comet assay) of Escherichia coli and carp (Cyprinus carpio) were performed for three pharmaceutically active compounds, ciprofloxacin, 17α-ethinylestradiol and 5-fluorouracil, used in the treatment of humans. The values of genotoxicity induction coefficient (I) in the SOS chromotest clearly showed genotoxicity for ciprofloxacin, both in the presence and in the absence of S9 fraction; 17α-ethinylestradiol demonstrated slight genotoxicity at the highest tested concentration; and 5-fluorouracil did not induce genotoxic effects in Escherichia coli mutants. Statistical analysis of the results of the comet assay revealed significant differences in cell populations derived from carp placed in a solution of 5-fluorouracil in comparison with the negative control. Statistical analysis also showed a significant increase of "% DNA in tail" of comets in cell populations incubated in solutions of 17α-ethinylestradiol at concentrations of 10000, 2000 and 400 µg/L and in solutions of 5-fluorouracil with S9 fraction at concentrations of 50,000 and 2,000 μg/L in comparison with the negative controls.
- Published
- 2016
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