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Phosphorus (P) is involved in various biochemical reactions in plant growth, so it is beneficial to plants growing in soils contaminated by metals, including cadmium (Cd). However, few studies have reported on the mechanistic roles of P in mitigating Cd toxicity to ryegrass root, and especially in alleviating the disruption of the mitochondrial function of living cells. In this study, the physiological and biochemical mechanisms associated with ryegrass growth under various Cd and P treatments were investigated using leaching and pot systems. The concentration of Cd in soil leachates showed a significantly positive relationship with redox potential (P < 0.05), but negative relationship (P < 0.05) with leachate pH values and dissolved organic carbon (DOC), indicating that exogenous P addition (as H 2 PO 4 −) may decrease Cd leaching from contaminated soil. Compared to the control (without P addition), the cumulative Cd content was reduced by 53.3% and 64.5% in the soil leachate with exogenous P application (20 mg/L and 80 mg/L), respectively. Notably, application of P decreased the Cd concentrations in the symplastic fractions and increased the Cd concentrations in the apoplastic fractions in root tips, which may help to alleviate Cd stress to the protoplast. Moreover, exogenous P was found to play a positive role in mitochondrial function and Ca2+ variation in root cells under Cd stress, which provides novel insights into the mechanisms of exogenous P in alleviating plant Cd injury. [Display omitted] [ABSTRACT FROM AUTHOR]