1. Triiodothyronine Has No Enhancement Effect on the Osteogenic or Chondrogenic Differentiation of Equine Adipose Tissue Stem Cells
- Author
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Debora de oliveira spila, Jorge Tiburcio Barbosa de Lima, Jankerle Neves Boeloni, Renata de Pino Albuquerque Maranhão, Rogéria Serakides, Natália de Melo Ocarino, and Fabrício Gomes Melo
- Subjects
Triiodothyronine ,040301 veterinary sciences ,Equine ,Chemistry ,Stem Cells ,Mesenchymal stem cell ,0402 animal and dairy science ,Adipose tissue ,Cell Differentiation ,04 agricultural and veterinary sciences ,Chondrogenesis ,040201 dairy & animal science ,0403 veterinary science ,Andrology ,Adipose Tissue ,Cell culture ,Animals ,Alkaline phosphatase ,Horses ,Viability assay ,Stem cell ,Cells, Cultured - Abstract
The effects of two concentrations of triiodothyronine (T3; 0.01 and 1,000 nM) on the osteogenic and chondrogenic differentiation abilities of equine adipose-derived mesenchymal stem cells (AD-MSCs) were evaluated. The osteogenic study evaluated the effect of T3 using alkaline phosphatase activity (ALP) assay; cell viability and density; and formation of mineralized nodules at Days 7, 14, and 21 in culture. The chondrogenic study tested the effect of T3 through ALP assay, mitochondrial metabolism, cell density, and periodic acid-Schiff-positive (PAS+) matrix percentage at Days 7 and 14. In both experiments, analysis of variance was used to compare averages through the Student-Newman-Keuls test. In the osteogenic study, no differences in any variable were detected between groups at Day 7. At Day 14, 0.01 nM T3 reduced cell density and the number of mineralized nodules despite the increase in ALP activity and mitochondrial metabolism (P.05). ALP activity increased at 1,000 nM T3 concentration (P.05). At Day 21, 0.01 nM T3 treatment increased ALP activity compared with control treatment (P.05). At 1,000 nM concentration, T3 reduced mitochondrial metabolism and cell density (P.05). In the chondrogenic study, the two T3 concentrations increased cell density compared with control treatment at Day 7. At Day 14, higher T3 concentration reduced mitochondrial metabolism, ALP activity, cell density, and PAS+ chondrogenic matrix percentage compared with control treatment (P.05). Thus, T3 addition to equine AD-MSC cultures has no enhancement effect on osteogenic or chondrogenic differentiation and may, in fact, negatively affect cell density and matrix synthesis depending on hormone concentration and culture time.
- Published
- 2020