3 results on '"Zhang, LI‐Jun"'
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2. Anti-inflammatory effect of Man-Pen-Fang, a Chinese herbal compound, on chronic pelvic inflammation in rats.
- Author
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Zhang, Li-Jun, Zhu, Jian-Yong, Sun, Meng-Yao, Song, Ya-Nan, Rahman, Khalid, Peng, Cheng, Zhang, Miao, Ye, Yu-Mei, and Zhang, Hong
- Subjects
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ANTI-inflammatory agents , *BIOLOGICAL models , *ENZYME-linked immunosorbent assay , *HERBAL medicine , *INTERLEUKINS , *CHINESE medicine , *PELVIC inflammatory disease , *POLYMERASE chain reaction , *RATS , *RNA , *TRANSFORMING growth factors-beta - Abstract
Ethnopharmacological relevance Traditional Chinese Medicine (TCM) has become the focus of research for the treatment of chronic pelvic inflammatory disease (CPID) based on unique medical theory system. Man-Pen-Fang (MPF), a Chinese herbal compound, which is composed of Thlaspi arvense L. (Cruciferae), Gleditsia sinensis Lam. (Leguminosae), Smilax china L. (Liliaceae), Euonymus alatus (Thunb.) Sieb. (Celastraceae) and Vaccaria segetalis (Neck.) (Caryophyllaceae) MPF has been used for the treatment of CPID and exerted significant clinical curative effects. However, the corresponding active principles and anti-inflammatory mechanism of MPF are still unknown. Aim of the study The objective of present study is to evaluate the effect of MPF on CPID in the chronic pelvic inflammation (CPI) rat model and elucidate its possible anti-inflammatory mechanism. Materials and Methods The CPI in rats was induced by administration with E. coli, Staphylococcus aureus and Beta-hemolytic streptococcus. MPF (8.112 g/(kg d) (20 times of adult dosage), 4.056 g/(kg d) (10 times of adult dosage) and 2.028 g/(kg d) (5 times of adult dosage)) and Jingangteng Capsule 2 g/(kg d) (20 times of adult dosage) were administered orally for 20 days. The serum levels of five inflammation-associated cytokines (IL-2, IL-6, IL-10, TNF-α and TGF-β 1 ) were determined by enzyme-linked immunoassay, and the mRNA expression levels of TGF-β 1 , P53, Fas, FasL and MMP-2 in the uterus tissue were measured by quantitative RT-PCR. Furthermore, the expression of NF-κB p65 in uterus and ovary tissues was detected by immunohistochemistry assay and the pathological changes induced in the uterus and ovary tissues were observed by histology. Results MPF caused a reduction in serum levels of IL-2, IL-6, IL-10, TNF-α and TGF-β 1 . The expression of P53 mRNA, Fas/FasL mRNA and MMP-2 mRNA in the uterus tissue was significantly elevated after treating with MPF, in contrast the expression of TGF-β 1 mRNA was decreased. Furthermore, the expression of NF-κB p65 in uterus and ovary tissue was inhibited after treating with MPF. Conclusions These results taken together suggest that MPF has a significant anti-CPID effect, probably due to inhibition of the inflammation reaction by the promotion, and the induction of the apoptosis of inflammatory cells and downregulation of the serum levels of inflammation cytokines. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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3. Therapeutic effect of Dongbai-Tonglin-Fang, a Chinese herbal formula, on urinary tract infection in rat model.
- Author
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Li, Xiao-Qin, Zhu, Jian-Yong, Pan, Rong-Rong, Shen, Yu-Li, Rahman, Khalid, Zhang, Chun-Yan, Zhang, Li-Jun, Luan, Xin, and Zhang, Hong
- Subjects
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TISSUE analysis , *ANALGESICS , *ANIMAL experimentation , *ANTI-inflammatory agents , *BILIRUBIN , *FECAL occult blood tests , *HERBAL medicine , *HISTOLOGICAL techniques , *KETONES , *KIDNEYS , *LEUCOCYTES , *CHINESE medicine , *NITRITES , *RATS , *STAINS & staining (Microscopy) , *TISSUES , *URINARY tract infections , *ALBUMINS , *TREATMENT effectiveness , *DRUG administration , *DRUG dosage , *PHARMACODYNAMICS , *THERAPEUTICS - Abstract
Traditional Chinese Medicine (TCM) has many obvious advantages in the treatment of chronic conditions such as urinary tract infection (UTI). Dongbai-Tonglin-Fang (DBTL), a Chinese herbal formula, has been used for the treatment of UTI for more than 40 years with proven efficacy. However, its mechanism of action is still unknown. The purpose of this study is to evaluate the therapeutic efficacy of DBTL and its mechanism of action in a rat UTI model. E. coli solution induced UTI rat model was used to evaluate the therapeutic effect of DBTL on UTI. Biochemical indicators related to UTI were measured. The kidney tissue was stained with hematoxylin-eosin (HE) to observe pathological changes whilst the ear swelling, feet swelling, hot plate and body torsion tests were used to estimate the anti-inflammatory and analgesic effects of DBTL. After treatment with different doses of DBTL (1, 2, 4 g/kg), a decrease in weight of the kidney in the UTI rat model was observed. The contents of white blood cell, nitrite, urinary albumin, ketone body, bilirubin and occult blood in the urine were also reduced whilst an increase in the pH of urine was observed. HE staining showed that the pathological changes in the kidney tissue were alleviated. At the same time, ear swelling assay showed that the weight and the degree of swelling of the ear of the mice in DBTL groups were decreased remarkably. DBTL also reduced the degree of feet swelling of the rats caused by the adjuvant. Furthermore, with the DBTL treatment, the latency period of foot licking induced by thermal stimulation was increased while the number of twists was lessened. These results show that DBTL has an excellent therapeutic effect on UTI rats accompanying with anti-inflammation and analgesia. The data presented here lays the foundations for further investigations in the treatment of UTI. Image 1 [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
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