1. Inhibitory effects of Tabebuia impetiginosa inner bark extract on platelet aggregation and vascular smooth muscle cell proliferation through suppressions of arachidonic acid liberation and ERK1/2 MAPK activation
- Author
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Sung-Keun Chang, Young-Hyun Park, Yeo-Pyo Yun, Gary R. Takeoka, Byeoung-Soo Park, Jeong-Han Kim, Mi-Ran Kim, Kwang-Geun Lee, Dong Ju Son, Yong Lim, Jin-Tae Hong, and Sung-Eun Lee
- Subjects
MAPK/ERK pathway ,Vascular smooth muscle ,Platelet Aggregation ,Myocytes, Smooth Muscle ,Biology ,Tabebuia ,Muscle, Smooth, Vascular ,chemistry.chemical_compound ,Drug Discovery ,Animals ,Platelet ,Extracellular Signal-Regulated MAP Kinases ,Cell Proliferation ,Pharmacology ,Arachidonic Acid ,DNA synthesis ,Cell growth ,Molecular biology ,Rats ,chemistry ,Biochemistry ,biology.protein ,Plant Bark ,Liberation ,Arachidonic acid ,Plant Preparations ,Rabbits ,Platelet-derived growth factor receptor - Abstract
The antiplatelet and antiproliferative activities of extract of Tabebuia impetiginosa inner bark (taheebo) were investigated using washed rabbit platelets and cultured rat aortic vascular smooth muscle cells (VSMCs) in vitro. n-Hexane, chloroform and ethyl acetate fractions showed marked and selective inhibition of platelet aggregation induced by collagen and arachidonic acid (AA) in a dose-dependent manner. These fractions, especially the chloroform fraction, also significantly suppressed AA liberation induced by collagen in [(3)H]AA-labeled rabbit platelets. The fractions, especially the chloroform fraction, potently inhibited cell proliferation and DNA synthesis induced by platelet derived growth factor (PDGF)-BB, and inhibited the levels of phosphorylated extracellular signal regulated kinase (ERK1/2) mitogen activated protein kinase (MAPK) stimulated by PDGF-BB, in the same concentration range that inhibits VSMC proliferation and DNA synthesis.
- Published
- 2005