10 results on '"Hong, Xu"'
Search Results
2. Danggui Buxue decoction ameliorates mitochondrial biogenesis and cognitive deficits through upregulating histone H4 lysine 12 acetylation in APP/PS1 mice
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Chai, Gao-shang, Gong, Juan, Wu, Jia-jun, Ma, Rui-kun, Zhu, Jun, Jia, Dong-dong, Zhang, Yu-qi, Zhai, Xiao-run, Sun, Hong-xu, Nie, Yun juan, Zhao, Peng, Xu, Yi-liang, and Yu, Hai tao
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- 2023
- Full Text
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3. Huaier suppresses cisplatin resistance in non-small cell lung cancer by inhibiting the JNK/JUN/IL-8 signaling pathway
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Jin, Haoyi, primary, Liu, Changhao, additional, Liu, Xi, additional, Wang, Huan, additional, Zhang, Yi, additional, Liu, Yu, additional, Li, Jijia, additional, Yu, Zhanwu, additional, and Liu, Hong-xu, additional
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- 2023
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4. Danggui Buxue decoction ameliorates mitochondrial biogenesis and cognitive deficits through upregulating histone H4 lysine 12 acetylation in APP/PS1 mice
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Gao-shang Chai, Juan Gong, Jia-jun Wu, Rui-kun Ma, Jun Zhu, Dong-dong Jia, Yu-qi Zhang, Xiao-run Zhai, Hong-xu Sun, Yun juan Nie, Peng Zhao, Yi-liang Xu, and Hai tao Yu
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Pharmacology ,Drug Discovery - Published
- 2023
5. Comparative and mechanistic study of pharmacodynamic difference in anti-breast cancer activity between water and liquor extracts of Xiaojin Pills
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Huimin Liu, Bo Cao, Chunyu Li, Li Han, Hong Xu, Junzhi Lin, Dingkun Zhang, and Runchun Xu
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Pharmacology ,Water ,Apoptosis ,Breast Neoplasms ,Mice ,Phosphatidylinositol 3-Kinases ,Drug Discovery ,Animals ,Humans ,Female ,Phosphatidylinositol 3-Kinase ,Proto-Oncogene Proteins c-akt ,Cell Proliferation ,Drugs, Chinese Herbal - Abstract
Xiaojin Pills was first recorded during the Qing Dynasty and have a history of nearly 300 years. It is the first choice among Chinese patent medicines for the clinical treatment of diseases of the mammary glands in contemporary traditional Chinese medicine. It was also widely used in the treatment of breast cancer, lung cancer, thyroid cancer, and other malignant tumors. Its initial administration method was "taken orally after soaking with Chinese baijiu"; however, the method was changed to "taken orally with water" within the last 40 years. There is no scientific evidence for the difference in efficacy against breast cancer between the two methods of administration.In vitro and in vivo experiments were carried out to confirm the therapeutic advantages of the liquor extract of Xiaojin Pills to improve the efficacy against breast cancer, and the mechanism was explained in terms of metabolomics and molecular biology.In vitro, a cell counting kit-8 cell activity assay and flow cytometry were used to detect the activity and apoptosis of MCF-7 breast cancer cells. In vivo, pharmacodynamic evaluation was performed by constructing a heterotopic transplantation model of breast cancer in BALB/c-nu mice. TUNEL staining was used to observe the apoptosis of cells in tumor tissues. The expression of proteins associated with the phosphoinositide 3-kinase (PI3K)/Akt pathway in BALB/c-nu mice tissue was investigated by metabolomics analysis, immunohistochemistry and western blotting.CCK-8 assay showed that the ICThis study demonstrates that the liquor extract of Xiaojin Pills had a stronger anti-breast cancer effect than that of the water extract. The PI3K/Akt signaling pathway might play an important role in the mechanism of the liquor extract of Xiaojin Pills and thus improve the efficacy against breast cancer.
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- 2021
6. Comparative study on the pharmacodynamic difference of oral administration of Xiaojin Pills accompanied with Chinese Baijiu and water
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Fei Ran, Run-Chun Xu, Junzhi Lin, Bi Feng, Li Han, Min Qiu, Bo Cao, Jiao Song, Ding-Kun Zhang, and Hong Xu
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Male ,Linoleic acid ,Analgesic ,Mammary Gland Tissue ,Anti-Inflammatory Agents ,Administration, Oral ,Pain ,Traditional Chinese medicine ,Pharmacology ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Oral administration ,Drug Discovery ,medicine ,Animals, Outbred Strains ,Animals ,Metabolomics ,Medicine, Chinese Traditional ,030304 developmental biology ,Inflammation ,0303 health sciences ,Analgesics ,Hyperplasia ,business.industry ,Alcoholic Beverages ,Water ,Drug Synergism ,Oryza ,medicine.disease ,Rats ,Disease Models, Animal ,chemistry ,030220 oncology & carcinogenesis ,Pharmacodynamics ,Female ,Animal studies ,business ,Drugs, Chinese Herbal - Abstract
Ethnopharmacological relevance Xiaojin Pills is a classic prescription for the treatment of mammary glands hyperplasia with a history of nearly 300 years, and is also the first choice of Chinese patent medicine for the clinical treatment of mammary glands hyperplasia in contemporary traditional Chinese medicine clinic. Clinical and animal studies have shown that Xiaojin Pills has the effects of anti-mammary glands hyperplasia, promoting blood circulation, anti-inflammation and analgesia. However, its initial administration method was “taking orally after soaked with Chinese Baijiu”, the modern method was changed to “taking orally with water” in recent 20 years. Whether there is any difference in the efficacy of the two administration methods is still unknown. Aim of the study To reveal the difference in efficacy and metabolic mechanism of anti-mammary gland hyperplasia between the oral administration of Xiaojin Pills accompanied with Chinese Baijiu (XJP&B) and water (XJP&W). Materials and methods COX-2 inhibition rate test and anti-platelet aggregation activity test were used to investigate the efficacy difference between the 40 vol% Chinese Baijiu and water extracts of Xiaojin Pills on anti-inflammatory and blood-activating in vitro. Kunming male mice (20 ± 5 g) and SD female rats (200–220 g) were orally treated with XJP&B and XJP&W, respectively. Then the difference in anti-inflammatory and analgesic effects between XJP&B and XJP&W were evaluated via xylene-induced ear swelling test, formaldehyde-induced pain test, and acetic acid-induced writhing test in mice. Determination of nipple diameter, pathological examination of mammary gland tissue, determination of serum E2, P and FSH content and hemorheological parameters of rats with mammary gland hyperplasia were performed to explore the efficacy difference in anti-mammary gland hyperplasia between XJP&B and XJP&W. Metabolomics was used to study the difference of anti-mammary gland hyperplasia mechanism between XJP&B and XJP&W. Results The results showed that the effect of XJP&B was superior to that of XJP&W in anti-platelet aggregation, inhibition of inflammation and pain, and anti-mammary gland hyperplasia. Interestingly, the advantages were more significant under low-dose condition. In addition, the mechanism of the two combinations against mammary gland hyperplasia was indeed different. Their common metabolic pathways include tryptophan metabolism and alanine, aspartic acid and glutamic acid metabolism. However, Chinese Baijiu and XJP&B also have additional regulatory effects on linoleic acid metabolic pathway. Conclusion In brief, this research demonstrated that the efficacy of XJP&B was better than that of XJP&W in activating the blood, anti-inflammation, analgesia and anti-mammary gland hyperplasia, which means that XJP&B has synergistic and superior effects. The special dose-effect relationship under the condition of XJP&B was also found, laying the foundation for clinical treatment to reduce the dosage and shorten the medication cycle, which is beneficial to reduce the economic burden of patients.
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- 2021
7. Study on the protection of water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata against NAFLD and its mechanism
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Shaobo Zhang, Liang Shi, Hong Xu, Zhanxia Hao, Bin Lu, Lili Ji, and Li Lin
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Male ,Resveratrol ,Pharmacology ,Plant Roots ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,NEFA ,Non-alcoholic Fatty Liver Disease ,Drug Discovery ,Nonalcoholic fatty liver disease ,medicine ,Oil Red O ,Animals ,Aspartate Aminotransferases ,Rats, Wistar ,030304 developmental biology ,chemistry.chemical_classification ,Liver injury ,0303 health sciences ,Carnitine O-Palmitoyltransferase ,Plant Extracts ,Fatty acid ,Water ,Alanine Transaminase ,medicine.disease ,chemistry ,Liver ,030220 oncology & carcinogenesis ,Solvents ,Polygonum ,Emodin ,Reactive Oxygen Species ,Etomoxir - Abstract
Ethnopharmacological relevance Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Praeparata (PMRP) that is used after processing are two well-known traditional Chinese medicines. PMRP is traditionally reported to have lipid-reducing activity as recorded in Chinese Pharmacopoeia. Aim of the study: This study aims to observe the alleviation of Polygoni Multiflori Radix Praeparata water extract (PMRPWE) and Polygoni Multiflori Radix water extract (PMRWE) against nonalcoholic fatty liver disease (NAFLD), and its potential engaged mechanism and the main active ingredients. Materials and methods The contents of 2,3,5,4′-tetrahydroxy-stilbene-2-O-β- D-glucoside (TSG), emodin and physcion in PMRWE and PMRPWE were measured by using high-performance liquid chromatography (HPLC). NAFLD was induced in rats by high-fat diet (HFD) feeding for 8 weeks. At the same time, rats were orally given with PMRWE (70, 140, 280 mg/kg) or PMRPWE (70, 140, 280 mg/kg) every day. Serum and liver biochemical parameters, hepatic gene expression and enzymatic activity were detected. Cellular lipids accumulation in human normal liver L-02 cells was induced by 0.5 mM non-esterified fatty acid (NEFA). Results The results of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver reactive oxygen species (ROS) and hematoxylin-eosin (H&E) observation showed that PMRWE and PMRPWE both alleviated liver injury in HFD-fed rats. The results of liver triglyceride (TG), total cholesterol (TC) and NEFA amounts, and liver Oil Red O staining evaluation showed that PMRWE and PMRPWE both reduced hepatic lipids accumulation in HFD-fed rats. The results of 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY) fluorescence staining and cellular TG content showed that both PMRWE and PMRPWE reduced NEFA-induced cellular lipids accumulation in L-02 cells. PMRWE and PMRPWE increased liver mRNA expression of some signals involved in mitochondrial β oxidation, including the key enzyme carnitine palmitoyltransferase 1A (CPT1A). Moreover, PMRWE and PMRPWE increased the decreased liver CPT1A enzymatic activity in HFD-fed rats. Etomoxir (ETO), a CPT1A inhibitor, weakened the lipid-lowering activity of PMRWE and PMRPWE in vitro. Additionally, the main compounds in PMRWE and PMRPWE including TSG, emodin, physcion and resveratrol all reduced cellular lipids accumulation induced by NEFA in L-02 cells. Conclusions PMRWE and PMRPWE alleviated NAFLD through promoting mitochondrial β oxidation by enhancing liver CPT1A activity. Stilbenes (including TSG, polydatin and resveratrol) and anthraquinones (including physcion, emodin and rhein) may be the main active compounds contributing to the lipid-lowering activity provided by PMRWE and PMRPWE.
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- 2019
8. Da-Bu-Yin-Wan and Qian-Zheng-San, two traditional Chinese herbal formulas, up-regulate the expression of mitochondrial subunit NADH dehydrogenase 1 synergistically in the mice model of Parkinson's disease
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Xin He, Xiao-Gang Gong, Hai-Xia Wu, Hong-Mei Sun, Hong Xu, Yuan-Yuan Wang, Yi Zhang, Zhen-Yu Guo, and Yushan Gao
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Male ,Mitochondrial DNA ,Tyrosine 3-Monooxygenase ,Neurotoxins ,Substantia nigra ,Striatum ,Pharmacology ,DNA, Mitochondrial ,Neuroprotection ,Mice ,chemistry.chemical_compound ,Drug Discovery ,Monoaminergic ,Animals ,Medicine ,Biogenic Monoamines ,RNA, Messenger ,Cerebral Cortex ,Behavior, Animal ,biology ,Tyrosine hydroxylase ,business.industry ,MPTP ,Intracellular Signaling Peptides and Proteins ,NADH dehydrogenase ,Proteins ,Drug Synergism ,Parkinson Disease ,Mice, Inbred C57BL ,Substantia Nigra ,Disease Models, Animal ,Neuroprotective Agents ,chemistry ,1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine ,biology.protein ,business ,Drugs, Chinese Herbal - Abstract
Ethnopharmacological relevance Da-Bu-Yin-Wan (DBYW) and Qian-Zheng-San (QZS), two traditional Chinese herbal formulas, were clinically employed to treat Parkinson's disease (PD) for decades. Aim of the study Our previous studies demonstrated neuroprotective effects of DBYW and QZS on mitochondrial function in mice model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). In present research, we aimed to investigate the possible neuroprotective mechanisms of DBYW and QZS. Materials and methods The effects of DBYW and QZS on the behavioral changes (pole test), expression of tyrosine hydroxylase (TH) of substantia nigra by immunohistochemistry, monoaminergic contents and activity of striatum by high performance liquid chromatography, neuronal ultrastructure changes by transmission electron microscopy, mitochondrial DNA (mtDNA) damage by long-extension polymerase chain reaction (PCR), and mRNA expression of mitochondrial subunit NADH dehydrogenase 1(ND1) by qualitative real-time PCR were investigated. Results Present study demonstrated that DBYW and QZS not only ameliorated the behavior induced by the administration of MPTP and synergistically prevented the decreasing of TH expression, but also increased monoaminergic contents and activity, improved the ultrastructural changes, decreased the mtDNA damage, and synergistically up-regulated the expression of ND1 in mRNA level. Conclusions These results suggest that DBYW and QZS possess anti-parkinsonism and neuroprotective properties.
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- 2013
9. Er Zhi Wan, an ancient herbal decoction for woman menopausal syndrome, activates the estrogenic response in cultured MCF-7 cells: an evaluation of compatibility in defining the optimized preparation method
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Wen Huang, Zhengtao Wang, Hong Xu, David T W Lau, Karl Wah Keung Tsim, Roy Chi Yan Choi, Yuzhong Zheng, Zi-Ren Su, and Tina T. X. Dong
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medicine.drug_class ,Genetic Vectors ,Ligustrum ,Decoction ,Breast Neoplasms ,Phytoestrogens ,Transfection ,complex mixtures ,Preparation method ,Cell Line, Tumor ,Drug Discovery ,Medicine ,Humans ,MTT assay ,Cell Proliferation ,Pharmacology ,Traditional medicine ,business.industry ,Cell growth ,Menopausal Syndrome ,Eclipta ,MCF-7 ,Receptors, Estrogen ,Estrogen ,MCF-7 Cells ,Female ,Plant Preparations ,Menopause ,business ,Drugs, Chinese Herbal - Abstract
Ethnopharmacological relevance Er Zhi Wan (EZW), a Chinese medicinal preparation, has been used clinically for treating menopausal syndrome for its kidney-invigorating function, which contains simply two herbs, Ecliptae Herba (EH) and Ligustri Lucidi Fructus (LLF). Although this herbal extract has been used for many years, there is no scientific basis about its effectiveness on menopausal symptom. Here, we aimed to evaluate the estrogenic activities of EZW and to study the compatibilities of two herbs including different processed-LLF in single and mixed preparation of EZW. Moreover, the weight ratio of EH to LLF in EZW was determined according to their estrogenic activities. Materials and methods The extractions of LLF, processed-LLF and EH were prepared separately by extracting the powders with water, 50% alcohol or 95% alcohol. Steamed-LLF and EH were extracted separately, or together, in preparing EZW extracts. A promoter-reporter construct (pERE-Luc) containing three repeats of estrogen responsive elements (ERE) was stably transfected into MCF-7 cells, and this stable breast cancer cell line was used to determine the estrogenic property. The cell proliferation was measured by MTT assay. Results The results showed that EZW could significantly induce the expression of luciferase driven by an estrogen responsive element in a pERE-Luc vector. The proliferation of MCF-7 cells was not altered by this herbal treatment. The best preparation of EZW was from: (i) LLF was firstly steamed over water and then dried to make steamed-LLF; and (ii) steamed-LLF and EH were extracted separately by 95% alcohol and then mixed together according to a weight ratio of 1:1. Conclusions Under the optimized extracting method, EZW possessed robust effect in activating the estrogenic activity, but which did not alter the proliferation of cultured MCF-7 cells. Thus, EZW is an effective and safe estrogenic herbal extract.
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- 2012
10. Involvement of p38 MAPK signaling pathway in the anti-melanogenic effect of San-bai-tang, a Chinese herbal formula, in B16 cells
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Zhi-Ling Yu, Wang-Fun Fong, Hong Xu, Gui-Xin Chou, Hui Wang, Alexander Kai-Man Leung, Yan Ye, and Jian-Hong Chu
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MAP Kinase Signaling System ,Tyrosinase ,p38 mitogen-activated protein kinases ,Melanoma, Experimental ,Pharmacology ,Paeonia ,p38 Mitogen-Activated Protein Kinases ,Melanin ,Cell Line, Tumor ,Drug Discovery ,Medicine ,Humans ,MTT assay ,Viability assay ,Enzyme Inhibitors ,Cytotoxicity ,Melanins ,Traditional medicine ,business.industry ,Monophenol Monooxygenase ,Atractylodes ,Microphthalmia-associated transcription factor ,Cell culture ,Smilax ,business ,Drugs, Chinese Herbal ,Phytotherapy - Abstract
Aim of the study San-bai-tang (SBT), a Chinese herbal formula, is traditionally used as a skin whitener in China. In our previous screening assays, SBT was identified as an effective tyrosinase inhibitor. In this study, we aim to investigate the anti-melanogenic effect and mechanisms of SBT in B16 cells. Materials and methods Cell viability was examined by the MTT assay. Cellular tyrosinase activity and melanin content were determined using spectrophotographic methods. Protein expression was analyzed by immunoblotting. Results SBT inhibited tyrosinase activity with an IC50 of 215.6 ± 10.3 μg/ml, and decreased cellular melanin content with an IC50 of 254.8 ± 14.5 μg/ml at 48 h. MTT assay demonstrated that 48-h SBT (50–400 μg/ml) treatment did not show obvious cytotoxicity. Immunoblot analysis showed that SBT (100, 200 or 400 μg/ml) treatment for 48 h down-regulated the expression levels of phosphorylated-p38, MITF, tyrosinase, TRP-1 and TRP-2 in a dose-dependent manner. Conclusions SBT inhibited melanogenesis in B16 cells, and suppression of p38 MAPK signaling pathway contributed to the anti-melanogenic effect of SBT by down-regulating the expression of MITF and melanogenic enzymes. These novel findings demonstrated the anti-melanogenic effect and mechanisms of SBT, and provide pharmacological basis for the traditional use of SBT.
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- 2010
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