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Support for EU membership has long been an important topic of study. Individual-level research shows that winners of globalisation, including the higher educated, express greater EU support as they profit from economic and cultural conditions resulting from European integration. Recent context-focused work suggests that individuals in 'left-behind' places are less supportive of EU integration because of their home regions' weaker long-term economic conditions. This paper brings these two research strands into conversation with each other by examining how the relationship between EU support and individual-level education is contingent on subnational economic conditions. Using harmonised Eurobarometer data from almost 750,000 respondents spanning 2004–2019, combined with subnational economic data from 201 European regions, we find no evidence that subnational economic conditions influence the relationship between EU support and respondents' education level. At the same time, when cross-sectionally comparing long-term differences between regions, we find that EU support is positively related to regional GDP per capita (though unrelated to regional unemployment), among both the higher and lower educated, and especially in the post-Great Recession period. Longitudinally, EU support is positively related to declining regional unemployment, both among the higher and lower educated, but not to increasing regional GDP per capita. [ABSTRACT FROM AUTHOR]