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Giant honeybees, including the open-nesting Asian giant honeybee Apis dorsata, display a spectacular collective defence behaviour - known as 'shimmering' - against predators, which is characterised by travelling waves generated by individual bees flipping their abdomens in a coordinated and sequential manner across the bee curtain. We examined whether shimmering is visually mediated by presenting moving stimuli of varying sizes and contrasts to the background (dark or light) in bright and dim ambient light conditions. Shimmering was strongest under bright ambient light, and its strength declined under dim light in this facultatively nocturnal bee. Apis dorsata shimmered only when presented with the darkest stimulus against a light background, but not when this condition was reversed (light stimulus against dark background). This response did not attenuate with repeated exposure to the stimuli, suggesting that shimmering behaviour does not undergo habituation. We suggest that this is an effective anti-predator strategy in open-nesting A. dorsata colonies which are exposed to high ambient light, as flying predators are more easily detected when they appear as dark moving objects against a bright sky. Moreover, the stimulus detection threshold (smallest visual angular size) is much smaller in this anti-predatory context (1.6-3.4 deg) than in the context of foraging (5.7 deg), indicating that ecological context affects the visual detection threshold., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2022. Published by The Company of Biologists Ltd.)

Innate colour preferences in insects were long considered to be a non-flexible representation of a floral 'search image' guiding them to flowers during initial foraging trips. However, these colour preferences have recently been shown to be modulated by multi-sensory integration of information. Using experiments on the butterfly Catopsilia pomona (common emigrant), we demonstrate that cross-modal integration of information not only affects colour preferences but also colour learning, and in a sex-specific manner . We show that spontaneous colour preference in this species is sexually dimorphic, with males preferring both blue and yellow while females prefer yellow. With minimal training (two training sessions), both males and females learned to associate blue with reward, but females did not learn green. This suggests that the aversion to green, in the context of foraging, is stronger in females than in males, probably because green is used as a cue to find oviposition sites in butterflies. However, females learned green after extensive training (five training sessions). Intriguingly, when a floral odour was present along with green during training, female colour preference during the subsequent choice tests resembled their innate preference (preference for yellow). Our results show that multi-sensory integration of information can influence preference, sensory bias, learning and memory in butterflies, thus modulating their behaviour in a context-specific manner., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2020. Published by The Company of Biologists Ltd.)

Bees of the genus Apis are important foragers of nectar and pollen resources. Although the European honeybee, Apis mellifera, has been well studied with respect to its sensory abilities, learning behaviour and role as pollinators, much less is known about the other Apis species. We studied the anatomical spatial resolution and absolute sensitivity of the eyes of three sympatric species of Asian honeybees, Apis cerana, Apis florea and Apis dorsata and compared them with the eyes of A. mellifera. Of these four species, the giant honeybee A. dorsata (which forages during moonlit nights) has the lowest spatial resolution and the most sensitive eyes, followed by A. mellifera, A. cerana and the dwarf honeybee, A. florea (which has the smallest acceptance angles and the least sensitive eyes). Moreover, unlike the strictly diurnal A. cerana and A. florea, A. dorsata possess large ocelli, a feature that it shares with all dim-light bees. However, the eyes of the facultatively nocturnal A. dorsata are much less sensitive than those of known obligately nocturnal bees such as Megalopta genalis in Panama and Xylocopa tranquebarica in India. The differences in sensitivity between the eyes of A. dorsata and other strictly diurnal Apis species cannot alone explain why the former is able to fly, orient and forage at half-moon light levels. We assume that additional neuronal adaptations, as has been proposed for A. mellifera, M. genalis and X. tranquebarica, might exist in A. dorsata.