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Your search keyword '"Zhang, Huilong"' showing total 3 results
Start Over Author "Zhang, Huilong" Journal journal of experimental botany
3 results on '"Zhang, Huilong"'

1. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance

Author

Yao, Jun, primary, Shen, Zedan, primary, Zhang, Yanli, primary, Wu, Xia, primary, Wang, Jianhui, primary, Sa, Gang, primary, Zhang, Yuhong, primary, Zhang, Huilong, primary, Deng, Chen, primary, Liu, Jian, primary, Hou, Siyuan, primary, Zhang, Ying, primary, Zhang, Yinan, primary, Zhao, Nan, primary, Deng, Shurong, primary, Lin, Shanzhi, primary, Zhao, Rui, primary, and Chen, Shaoliang, primary

Published
2019
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2. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance.

Author

Yao, Jun, Shen, Zedan, Zhang, Yanli, Wu, Xia, Wang, Jianhui, Sa, Gang, Zhang, Yuhong, Zhang, Huilong, Deng, Chen, Liu, Jian, Hou, Siyuan, Zhang, Ying, Zhang, Yinan, Zhao, Nan, Deng, Shurong, Lin, Shanzhi, Zhao, Rui, and Chen, Shaoliang

Subjects
GENE expression, POPLARS, GENE silencing, CELL membranes, SALT
Abstract

Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter–reporter construct PeHA1-pro :: GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4 , which has a cis -acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis. [ABSTRACT FROM AUTHOR]

Published
2020
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3. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance.

Author

Yao J, Shen Z, Zhang Y, Wu X, Wang J, Sa G, Zhang Y, Zhang H, Deng C, Liu J, Hou S, Zhang Y, Zhang Y, Zhao N, Deng S, Lin S, Zhao R, and Chen S

Subjects
DNA-Binding Proteins genetics, Gene Expression, Gene Expression Regulation, Plant, Plant Proteins genetics, Plant Proteins metabolism, Plant Roots metabolism, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, Transcription Factors genetics, DNA-Binding Proteins metabolism, Populus genetics, Populus metabolism, Promoter Regions, Genetic, Proton-Translocating ATPases genetics, Salt Tolerance genetics, Transcription Factors metabolism
Abstract

Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis., (© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.)

Published
2020
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