1. The route of priming influences the ability of respiratory virus-specific memory CD8+ T cells to be activated by residual antigen.
- Author
-
Takamura S, Roberts AD, Jelley-Gibbs DM, Wittmer ST, Kohlmeier JE, and Woodland DL
- Subjects
- Administration, Intranasal, Animals, CD8-Positive T-Lymphocytes cytology, CD8-Positive T-Lymphocytes immunology, Cell Movement, Epitopes, Injections, Intraperitoneal, Lung virology, Lymph Nodes immunology, Lymph Nodes virology, Mediastinum virology, Mice, Species Specificity, Antigens, Viral immunology, CD8-Positive T-Lymphocytes virology, Cross-Priming immunology, Immunologic Memory immunology, Lung immunology, Orthomyxoviridae immunology, Sendai virus immunology
- Abstract
After respiratory virus infections, memory CD8+ T cells are maintained in the lung airways by a process of continual recruitment. Previous studies have suggested that this process is controlled, at least in the initial weeks after virus clearance, by residual antigen in the lung-draining mediastinal lymph nodes (MLNs). We used mouse models of influenza and parainfluenza virus infection to show that intranasally (i.n.) primed memory CD8+ T cells possess a unique ability to be reactivated by residual antigen in the MLN compared with intraperitoneally (i.p.) primed CD8+ T cells, resulting in the preferential recruitment of i.n.-primed memory CD8+ T cells to the lung airways. Furthermore, we demonstrate that the inability of i.p.-primed memory CD8+ T cells to access residual antigen can be corrected by a subsequent i.n. virus infection. Thus, two independent factors, initial CD8+ T cell priming in the MLN and prolonged presentation of residual antigen in the MLN, are required to maintain large numbers of antigen-specific memory CD8+ T cells in the lung airways.
- Published
- 2010
- Full Text
- View/download PDF