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Start Over Author "Heerklotz H" Journal journal of fluorescence
3 results on '"Heerklotz H"'

2. Surface area per molecule in lipid/C12En membranes as seen by fluorescence resonance energy transfer

Author

Lantzsch, G., Binder, H., and Heerklotz, H.

Abstract

Fluorescence resonance energy transfer (FRET) from NBD-PE to rhodamine-PE has been used to measure the average area occupied by surfactant molecules in lipid bilayers. The Foerster radius has been determined from the spectral overlap of donor fluorescence and acceptor absorption (R0=4.6 nm). The results of steady-state as well as of time-resolved investigations have been compared. The analysis of time-resolved fluorescence data by means of nonexponential decay functions yields an average area per lipid of 0.65 nm2 in pure POPC bilayers. The area per surfactant in two-component C12En/POPC-membranes (n=2, 4, 6) has been determined and compared with the results of X-ray investigations. The surfactant head group seems to adapt a predominantly disordered confirmation within the bilayer.

Published
1994
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3. Determination of the partition coefficients of the nonionic detergent C12E 7 between lipid-detergent mixed membranes and water by means of Laurdan fluorescence spectroscopy.

Author

Heerklotz H, Binder H, and Lantzsch G

Abstract

The membrane-water partition coefficient of the detergent C12E7 between water and C12E7/POPC mixed membranes has been determined by means of steady-state fluorescence spectroscopy. The emission spectra of the fluorescent probe Laurdan were used as an indicator of membrane composition at different membrane concentrations in the sample. The partition coefficient expressed as the ratio of the mole fractions of the detergent in the membrane and water phases is about 6(*)10(5) at low molar ratios of C12E7/POPC (R c ) and decreases rapidly with increasingR c . The limiting detergent content of the lamellar phase (R c (*) >≈0.8) is indicated by a minimum ofP(R c ).

Published
1994
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