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Your search keyword '"Polystyrene microsphere"' showing total 2 results
Start Over Descriptor "Polystyrene microsphere" Journal journal of fluorescence
2 results on '"Polystyrene microsphere"'

1. Quantum Dot-Based Luminescent Oxygen Channeling Assay for Potential Application in Homogeneous Bioassays.

Author

Zhuang, Si-Hui, Guo, Xin-Xin, Wu, Ying-Song, Chen, Zhen-Hua, Chen, Yao, Ren, Zhi-Qi, and Liu, Tian-Cai

Subjects
*BIOLOGICAL assay, *OPTICAL properties of quantum dots, *BIOTIN, *STREPTAVIDIN, *POLYSTYRENE, *MICROSPHERES, *PHOTOSENSITIZERS
Abstract

The unique photoproperties of quantum dots are promising for potential application in bioassays. In the present study, quantum dots were applied to a luminescent oxygen channeling assay. The reaction system developed in this study was based on interaction of biotin with streptavidin. Carboxyl-modified polystyrene microspheres doped with quantum dots were biotinylated and used as acceptors. Photosensitizer-doped carboxyl-modified polystyrene microspheres were conjugated with streptavidin and used as donors. The results indicated that the singlet oxygen that was released from the donor beads diffused into the acceptor beads. The acceptor beads were then exited via thioxene, and were subsequently fluoresced. To avoid generating false positives, a high concentration (0.01 mg/mL) of quantum dots is required for application in homogeneous immunoassays. Compared to a conventional luminescent oxygen channeling assay, this quantum dots-based technique requires less time, and would be easier to automate and miniaturize because it requires no washing to remove excess labels. [ABSTRACT FROM AUTHOR]

Published
2016
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2. [Untitled]

Author

Yu Zhong Zhang, Bruce P. Wittmershaus, Sharon Swan, Jamie J. Skibicki, and Jason B. McLafferty

Subjects
Sociology and Political Science, Clinical Biochemistry, Quantum yield, equipment and supplies, Photochemistry, Biochemistry, Fluorescence, Clinical Psychology, chemistry.chemical_compound, chemistry, Yield (chemistry), Polystyrene microsphere, SPHERES, Absorption (chemistry), BODIPY, Photodegradation, Law, Spectroscopy, Social Sciences (miscellaneous)
Abstract

The absorption, fluorescence, fluorescence quantum yield, and photostability of five BODIPY dyes are characterized and compared as single dyes in two environments, in 40-nm polystyrene spheres and in solution. The absorption and fluorescence spectra of the dyes in spheres are similar in profile but shifted to lower energies compared to those in solution. All the dyes are highly fluorescent, with three having fluorescence quantum yields of 1.0. For three of the five dyes, the yields were the same in spheres as in solution (1.00, 1.00, and 0.73). The high concentration of these dyes in spheres does not quench their fluorescence. For two other dyes the yields dropped, from 1.00 to 0.55 in one case and 0.83 to 0.50 in another, comparing the dyes in solution versus in spheres. The photodegradation of the dyes decreases in spheres compared to in solution in all but one case. For one dye, it decreases as much as 800-fold. Dyes overlooked because of low fluorescence or stability in solution could become useful fluorescent materials in the microsphere environment.

Published
2001
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