1. Differential proteomic analysis of frozen tilapia (Oreochromis niloticus) fillets with quality characteristics by a tandem mass tag (TMT)-based strategy.
- Author
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Wang, Hongli, Shi, Wenzheng, and Wang, Xichang
- Subjects
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TILAPIA , *NILE tilapia , *FISH fillets , *DENATURATION of proteins , *PROTEOMICS , *ICE crystals , *ANIMAL coloration , *COLOR of birds - Abstract
A tandem mass tag technology (TMT), based on the quantitative proteomics approach, was applied to investigate the associations between tilapia (Oreochromis niloticus) fillet quality characteristics (pH, color, texture, ice crystal morphology and protein denaturation) and proteome profiles during a 12-month frozen storage period. When compared with the control group (0 day), a total of 175 differentially abundant proteins (DAPs) were found in tilapia muscle from two frozen storage temperatures (−20 °C and −40 °C). Correlation analysis between the DAPs and the quality traits showed that 80 DAPs were correlated closely with the quality traits. The bioinformatics analysis revealed that 49, 9 and 2 DAPs were involved in binding proteins, metabolic enzymes and protein turnover, respectively, which were mainly closely associated with color traits and protein denaturation. These results provide a further understanding of the proteome changes and mechanisms of the quality decline of tilapia during frozen storage. • A TMT-based strategy was applied to the proteomics of frozen tilapia fillets for 12 months. • Tilapia fillets stored at − 40 °C exhibited satisfactory quality traits. • Tilapia fillets stored at − 20 °C showed greater proteome changes than those stored at − 40 °C. • Twenty-nine and 17 DAPs correlated closely with color trait and protein denaturation. • Four proteins could be potential biomarkers for monitoring the quality of frozen tilapia. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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