1. Global optimization for simultaneous extraction of oil and polysaccharides from Schizochytrium limacinum by enzyme-assisted three-phase partitioning.
- Author
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Wenwei Chen, Zhenbao Jia, Guangrong Huang, and Yao Hong
- Subjects
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GLOBAL optimization , *ALGAL biofuels , *POLYSACCHARIDES , *AMMONIUM sulfate , *MARINE algae , *EDIBLE fats & oils - Abstract
Marine algae are important sources of biological substances. Polysaccharides and oil in algae have a broad range of biological activities. The aim of our study was to develop a novel method for the extraction of polysaccharides and oil from Schizochytrium limacinum by enzyme-assisted three-phase partitioning. S. limacinum was pretreated by protamex hydrolysis for 2 hr, at pH 3.0, temperature of 45°C, and enzyme concentration of 2.0%. Response surface was used to analyze the three-phase extraction and Box–Behnken design was employed to model algae oil and polysaccharides. The following optimal conditions were obtained by global optimization: 34% mass fraction of ammonium sulfate, 1:2.4 ratio of slurry to t-butanol, 41 min time, 40°C temperature, 35.69% oil yield, and 5.16% polysaccharides yield. The polysaccharides exhibited potential antioxidant activity in DPPH, ABTS, and reducibility assay. The results indicated that the algal polysaccharides may be used as a natural antioxidant in food. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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