1. Comparison of Enrichment Procedures for Shiga Toxin–Producing Escherichia coli in Wastes from Commercial Swine Farms
- Author
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Mark Mogler, Michael A. Grant, and D. L. Harris
- Subjects
Swine ,Colony Count, Microbial ,Biology ,medicine.disease_cause ,Microbiology ,Shiga Toxin ,Food and drug administration ,Feces ,chemistry.chemical_compound ,Shiga-like toxin ,STX2 ,Enterotoxigenic Escherichia coli ,medicine ,Animals ,Humans ,Food science ,Escherichia coli ,Shiga toxin-producing Escherichia coli ,Shiga-Toxigenic Escherichia coli ,biology.organism_classification ,Enterobacteriaceae ,Culture Media ,chemistry ,Bacteria ,Food Science - Abstract
Three methods for enrichment of Shiga toxin-producing Escherichia coli (STEC) were compared using waste pit samples from swine production facilities housing 50 to 3,000 animals. The STEC gene stx2 was detected in 5 of 17 pooled samples using a U.S. Department of Agriculture (USDA) enrichment procedure, 6 of 17 samples using a U.S. Food and Drug Administration (FDA) enrichment procedure, and 8 of 17 samples using an experimental acid enrichment. All isolates were non-O157 and 5 of 6 were positive for enterotoxigenic E. coli-associated heat stable toxins a and b. The three enrichment procedures were also tested for their ability to support growth of 31 strains of STEC. The acid enrichment media supported growth of 100% of the strains, the FDA medium supported 77% of the strains, and the USDA medium supported 16% of the strains.
- Published
- 2009
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