Search

As a powerful alternative to short tandem repeat (STR) profiling, we have developed a novel panel of 47 single nucleotide polymorphisms (SNPs) for DNA profiling and ABO genotyping. We selected 42 of the 47 SNPs from a panel of 86 markers that were previously validated as universal individual identification markers and identified five additional SNPs including one gender marker and four ABO loci. Match probability of the 42 validated SNPs was found to be 9.5 × 10−18 in Han Chinese. SNP analysis correctly assessed a panel of historical cases, including both paternity identifications in trios and individual identifications. In addition, while STR profiling of degraded DNA provided information for 11 loci of 16 potential markers with low peak intensities, SNPstream® genotyping was sufficient to identify all 47 SNPs. In summary, SNP analysis is equally effective as STR profiling, but appears more suited for individual identification than STR profiling in cases where DNA may be degraded. [ABSTRACT FROM AUTHOR]

In forensic genetic analyses, mixtures of various biological materials are common samples. Micromanipulation, which is performed based on differences in cellular morphology, is an effective method for the isolation of cells from mixtures. In this study, mucosal cell was isolated from somatic cellular mixtures (blood and saliva) based on micromanipulation and a low volume- PCR ( LV- PCR) platform. One hundred and twenty-six parallel LV- PCR processes were performed using an Identifiler® kit, with 107 reactions yielding single-source DNA profiles. Among them, 54 full profiles (50%) and 37 partial profiles (13-15 loci) were obtained. Based on the above method, we obtained a single-source DNA profile from a cigarette butt contaminated by two victims' blood in a murder case. The generated genotype was used to query a DNA database, and a perfect match was found. [ABSTRACT FROM AUTHOR]