1. DNA Profiling Success Rates from Degraded Skeletal Remains in Guatemala
- Author
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Mishel Stephenson and Emma Johnston
- Subjects
0301 basic medicine ,Male ,Bone density ,Physiology ,Biology ,Bone and Bones ,Pathology and Forensic Medicine ,03 medical and health sciences ,chemistry.chemical_compound ,Forensic dna ,0302 clinical medicine ,Genetics ,Humans ,Femur ,030216 legal & forensic medicine ,Forensic anthropology ,DNA ,Guatemala ,DNA Fingerprinting ,Genealogy ,Body Remains ,Forensic science ,030104 developmental biology ,DNA profiling ,chemistry ,Microsatellite ,Forensic Anthropology ,Female ,Microsatellite Repeats - Abstract
The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link. No data are available regarding the success of DNA Short Tandem Repeat (STR) profiling from degraded skeletal remains in Guatemala. Therefore, DNA profiling success rates relating to 2595 skeletons from eleven cases at the Forensic Anthropology Foundation of Guatemala (FAFG) are presented. The typical postmortem interval was 30 years. DNA was extracted from bone powder and amplified using Identifiler and Minifler. DNA profiling success rates differed between cases, ranging from 50.8% to 7.0%, the overall success rate for samples was 36.3%. The best DNA profiling success rates were obtained from femur (36.2%) and tooth (33.7%) samples. DNA profiles were significantly better from lower body bones than upper body bones (p =
- Published
- 2014