1. Impaired function of CD4CD25 T regulatory lymphocytes characterizes the self-limited hepatitis A virus infection
- Author
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Costanza Sbreglia, Simona Altamura, Giuseppe Morelli, Alessandro Perrella, Stella Grattacaso, Luigi Racioppi, Luigi Atripaldi, Tommaso Patarino, Laura Vitiello, Oreste Perrella, Pasquale Bellopede, Perrella, A., Vitiello, Laura, Atripaldi, L., Sbreglia, C., Grattacaso, S., Bellopede, P., Patarino, T., Morelli, G., Altamura, S., Racioppi, Luigi, and Perrella, O.
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Adult ,Male ,CD3 Complex ,CD3 ,Lymphocyte Activation ,T-Lymphocytes, Regulatory ,DENDRITIC CELLS ,DISEASE ,Flow cytometry ,INFLAMMATION ,medicine ,Humans ,IMMUNE-RESPONSE ,IL-2 receptor ,Seroconversion ,Cells, Cultured ,SUPPRESSION ,Hepatitis ,Hepatology ,biology ,medicine.diagnostic_test ,business.industry ,Interleukin-2 Receptor alpha Subunit ,Gastroenterology ,Case-control study ,T helper cell ,Hepatitis A ,medicine.disease ,Hepatitis a virus ,medicine.anatomical_structure ,Case-Control Studies ,CD4 Antigens ,AUTOIMMUNE HEPATITIS ,Immunology ,biology.protein ,Female ,business ,Hepatitis A Virus, Human - Abstract
Background and Aim: Hepatitis A virus (HAV) causes a transient illness leaving permanent protection against reinfection. Few data are available on the regulatory mechanisms involved in the CD4+ T helper activation. We aimed to investigate the frequency and function of CD3+/CD4+/CD25+ T cells with regulatory function (Tregs) during acute HAV infection. Methods: We enrolled 35 consecutive patients and 15 healthy donors, enumerated Tregs by flow cytometry assay and evaluated, after immunomagnetical sorting with magnetic beads, their ability to inhibit the proliferation of CD4+/CD25– T lymphocytes at different ratios (1:1, 1:10, 1:20). Results: All patients had the usual course of infection. Our immunological analysis showed Tregs frequency in these patients (6.5% [range, 5–8.8%]; 36 [range, 10–87] cells) did not have any statistical difference compared with healthy donors (6% [range, 5–8%]; 48 (range, 23–71) cells), while their ability to suppress CD4+/CD25– was drastically reduced at different ratios (Mann–Whitney U-test; ratio 1:1, 93% vs 72%, z = −3.34, P
- Published
- 2008
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