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Shitouban Au deposit is a vein-type deposit, which located in the Dehua-Youxi area (Fujian Province, southeast China) and hosted by late Jurassic volcanic-sedimentary rocks. In this study, a detailed investigation on fluid inclusions, multiple isotopic compositions (H-O-S-Pb-Sr) and Rb Sr isotope dating of quartz have been carried out in an attempt to constrain the ore-forming processes and ore genesis. The deposit shares a characteristic of low-sulfidation epithermal Au deposit, such as low-temperature (peak T h 160–180 °C, average 168 °C) and low-salinity (2-3 wt% NaCl equiv., average 2.47). The hydrogen and calculated oxygen isotope values for fluids are −64 to −54‰ (average − 60‰) and - 0.95 to 0.76‰ (average 0.15‰) respectively, indicating that the ore-forming fluid was a mixture of dominant meteroric water and subsidiary magmatic fluid. Pyrite from the deposit shows a δ34S range from - 4.5 to - 1.2‰ (average −2.4‰), indicating a magmatic sulfur. The lead isotope ratios of pyrite, 206Pb/204Pb (18.399–18.494), 207Pb/204Pb (15.695–15.841), 208Pb/204Pb (38.842–39.297), are more radiogenic than the Jurassic magmatic rocks in the area, indicating a mixing metal source of magmatic and basement rocks. Based on the Rb Sr isotopic isochron age of Au-bearing quartz, the metallogenic epoch of Shitouban Au deposit is 157 ± 1 Ma, and consistent with the Jurassic volcanism in the Dehua-Youxi area. It is proposed that the primary hydrothermal fluids may have exsolved from the late Jurassic magma chamber and subsequently ascent into the shallower crust level near paleosurface to mix with meteoric water, which cause precipitation of Au and sulfides in the epithermal environment due to a decrease in metal solubility triggered by fluid boiling, cooling, and mixing. [Display omitted] • Shitouban Au deposit is a late Jurassic epithermal deposit in Dehua-Youxi area, Southeast China. • The ore-forming fluids show low-temperature and low-salinity characteristics. • H O isotopes indicate a mixture of dominant meteoric water with magmatic fluid. • Pb isotopes indicate a mixing metal source of magmatic and basement rocks. • Rb Sr geochronology of Au-bearing quartz yielded an age of 157 ± 1 Ma. [ABSTRACT FROM AUTHOR]