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Bitumens of the Alberta tar sands share a similar primary source rock, but exhibit varying levels of degradation. In the Peace River tar sands area, the wide range of API gravity and sulphur content in Lower Cretaceous reservoirs is investigated in terms of biodegradation processes, using detailed geochemical analyses, burial history modelling and charge/degrade modelling. Decreasing tar sand degradation to the west can be attributed to increasing reservoir temperatures which retard biodegradation. Gething oils west of the Peace River tar sands are derived from the same source, but are minimally degraded due to reservoir pasteurization at 80 °C shortly after charging. Long charge times, reservoir filling and possibly compositional or very low temperature degradation retardation have limited biodegradation of the Alberta tar sands despite long oil residence times and low reservoir temperatures.

Detailed petroleum system analysis allows the direction and extent of hydrocarbon flow to be assessed. In most parts of the Western Canada Sedimentary Basin, the major pulse of hydrocarbon generation and migration occurred as a result of the Laramide Orogeny in Late Cretaceous–Early Tertiary time. The distribution and chemistry of oils provide a detailed picture of fluid flow at this time with oil migration up to hundreds of kilometres observed. Hydrocarbon generation and migration mostly ceased once uplift of the basin commenced during Eocene time. As the basin evolved so did the hydrodynamic regime reflecting changes in topography, glaciation and other factors. This indicates possible problems with using present day hydrogeology to determine oil migration pathways although hydrogeology can help explain petroleum alteration.