1. DIET-like and MIET-like mutualism of S. oneidensis MR-1 and metal-reducing function microflora boosts Cr(VI) reduction.
- Author
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Jia, Boyu, Wan, Juanjuan, Liu, Hui, Yan, Bo, Zhang, Lijuan, and Su, Xintai
- Abstract
Microbial treatment of Cr(VI) is an environmentally friendly and low-cost approach. However, the mechanism of mutualism and the role of interspecies electron transfer in Cr(VI) reducing microflora are unclear. Herein, we constructed an intersymbiotic microbial association flora to augment interspecies electron transfer via functionalizing electroactive Shewanella oneidensis MR-1 with metal-reducing microflora, and thus the efficiency of Cr(VI) reduction. The findings suggest that the metal-reducing active microflora could converts glucose into lactic acid and riboflavin for S. oneidensis MR-1 to act as a carbon source and electron mediator. Thus, when adding initial 25 mg/L Cr (VI), this microflora exhibited an outstanding Cr (VI) removal efficiency (100%) at 12 h and elevated Cr (III) immobilization efficiency (80%) at 60 h with the assistance of 25 mg/L Cu(II). A series of electrochemical experiments proved this remarkable removal efficiency were ascribed to the improved interspecies electron transfer efficiency through direct interspecies electron transfer and riboflavin through mediated interspecies electron transfer. Furthermore, the metagenomic analysis revealed the expression level of the electron transport pathway was promoted. Intriguing high abundance of genes participating in the bio-reduction and biotransformation of Cr(VI) was also observed in functional microflora. These outcomes give a novel strategy for enhancing the reduction and fixation of harmful heavy metals by coculturing function microflora with electrogenic microorganisms. [Display omitted] • An intersymbiotic microflora was constructed by functionalizing S. oneidensis MR-1 with metal-reducing bacteria. • The microflora achieved Cr(VI) removal efficiency of 100% and Cr (III) immobilization efficiency of 80%. • The removal efficiency was ascribed to the improved interspecies electron transfer. • The metal-reducing active microflora converted glucose to lactate and riboflavin for S. oneidensis MR-1 to utilize. • The metagenomic analysis revealed the expression level of the electron transport pathways were promoted. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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