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Improvement of refractory nitrogen-containing organics biodegradation is crucial to meet discharged nitrogen standards and guarantee aquatic ecology safety. Although electrostimulation accelerates organic nitrogen pollutants amination, it remains uncertain how to strengthen ammonification of the amination products. This study demonstrated that ammonification was remarkably facilitated under micro-aerobic conditions through the degradation of aniline, an amination product of nitrobenzene, using an electrogenic respiration system. The microbial catabolism and ammonification were significantly enhanced by exposing the bioanode to air. Based on 16S rRNA gene sequencing and GeoChip analysis, our results indicated that aerobic aniline degraders and electroactive bacteria were enriched in suspension and inner electrode biofilm, respectively. The suspension community had a significantly higher relative abundance of catechol dioxygenase genes contributing to aerobic aniline biodegradation and reactive oxygen species (ROS) scavenger genes to protect from oxygen toxicity. The inner biofilm community contained obviously higher cytochrome c genes responsible for extracellular electron transfer. Additionally, network analysis indicated the aniline degraders were positively associated with electroactive bacteria and could be the potential hosts for genes encoding for dioxygenase and cytochrome, respectively. This study provides a feasible strategy to enhance nitrogen-containing organics ammonification and offers new insights into the microbial interaction mechanisms of micro-aeration assisted with electrogenic respiration. [Display omitted] • Aniline (AN) could be degraded under micro-aerobic bioanode system. • Microbial functional differentiation occurred in suspension and electrode. • Functional genes were more sensitive than 16S rRNA genes to DO stimulation. • AN degraders in suspension positively correlated with electroactive bacteria. • AN degraders were potential hosts of dioxygenase catalyzing ammonification. [ABSTRACT FROM AUTHOR]

The high concentrations of salt and refractory toxic organics in industrial wastewater seriously restrict biological treatment efficiency and functional stability. However, how to construct a salt-tolerant biocatalytic community and realize the decarbonization coupled with detoxification toward green bio-enhanced treatment, has yet to be well elucidated. Here, acetoacetanilide (AAA), an important intermediate for many dyes and medicine synthesis, was used as the model amide pollutant to elucidate the directional enrichment of halotolerant degradative communities and the corresponding bacterial interaction mechanism. Combining microbial community composition and molecular ecological network analyses as well as the biodegradation efficiencies of AAA and its hydrolysis product aniline (AN) of pure strains, the core degradative bacteria were identified during the hypersaline AAA degradation process. A synthetic bacterial consortium composed of Paenarthrobacter , Rhizobium , Rhodococcus , Delftia and Nitratireductor was constructed based on the top-down strategy to treat AAA wastewater with different water quality characteristics. The synthetic halotolerant consortium showed promising treatment ability toward the simulated AAA wastewater (AAA 100–500 mg/L, 1–5% salinity) and actual AAA mother liquor. Additionally, the comprehensive toxicity of AAA mother liquor significantly reduced after biological treatment. This study provides a green biological approach for the treatment of hypersaline and high concentration of organics wastewater. [Display omitted] • A halotolerant acetoacetanilide (AAA)-mineralizing consortium was obtained. • Core AAA-degrading strains were identified and isolated from the consortium. • Interspecific interactions drive biodegradation of AAA upon hypersaline stress. • Synthetic communities with bioremediation and stress resistance functions. [ABSTRACT FROM AUTHOR]