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Your search keyword '"Kamada, Takenobu"' showing total 15 results
Start Over Author "Kamada, Takenobu" Journal journal of hepatology
15 results on '"Kamada, Takenobu"'

3. Density analysis of hepatitis C virus particle population in the circulation of infected hosts: implications for virus neutralization or persistence

Author

Mita Eiji, Tatsuya Kanto, Kasahara Akinori, Tetsuo Takehara, Naito Masafumi, Fusamoto Hideyuki, Hayashi Norio, Kamada Takenobu, and Hagiwara Hideki

Subjects
Adult, Male, Adolescent, viruses, Hepatitis C virus, Population, Immunoglobulins, Hepacivirus, medicine.disease_cause, Liver disease, Immune system, medicine, Humans, Centrifugation, education, Aged, education.field_of_study, Hepatology, biology, Virion, Middle Aged, medicine.disease, Hepatitis C, Precipitin Tests, Virology, Reverse transcriptase, Immune complex, Antibodies, Anti-Idiotypic, Acute Disease, Chronic Disease, biology.protein, Female, Antibody
Abstract

Hepatitis C virus has a low buoyant density in sucrose, but high-density particles are often observed in hepatitis C virus infection. To investigate the characteristics of circulating hepatitis C virus particles and their association with liver disease progression, we examined sera from two histologically normal hepatitis C virus carriers, 20 chronic hepatitis patients and five acute hepatitis C patients. The supernatants obtained after immunoprecipitation with anti-immunoglobulins antibody were subjected to sucrose equilibrium centrifugation. HCV-RNA positive fractions separated after the treatments were further examined for immunoprecipitation with anti-core hepatitis C virus antibody. We separated hepatitis C virus particle populations according to the density difference on 35% sucrose with centrifugation. The proportions of high and low density particles in hepatitis C virus populations were determined by means of competitive reverse transcription and polymerase chain reaction. Circulating hepatitis C virus particles in chronically infected patients could be separated into two populations: those immunoglobulin-bound with high densities and -unbound with low densities. Patients with severe liver inflammation had high-density hepatitis C virus that did not precipitate with anti-immunoglobulins but with anti-core hepatitis C virus antibodies. Thus, hepatitis C virus particle populations consist of low-density virions and high-density immune complexes and/or nucleocapsids. Among the chronic hepatitis patients, the dominant population shifted from low-density to high-density particles with the progression of liver disease. In acute hepatitis patients, this density shift was observed with alanine aminotransferase normalizations. Therefore, the major hepatitis C virus populations change from virion to immune complex and/or nucleocapsid with the progression of liver disease or inflammation.

Published
1995
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14. Clinical applications of ICG Finger Monitor in patients with liver disease

Author

Ishigami, Yoshitaka, primary, Masuzawa, Manabu, additional, Miyoshi, Eiji, additional, Kato, Michio, additional, Tamura, Kazuya, additional, Kanda, Masahiko, additional, Awazu, Kunio, additional, Taniguchi, Kunihiro, additional, Kurita, Masahiro, additional, Hayashi, Norio, additional, Kawano, Sunao, additional, Fusamoto, Hideyuki, additional, and Kamada, Takenobu, additional

Published
1993
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