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The purpose of this study was to investigate the interval in which male rugby union players reach maximum speed in a 50 m sprint according to age categories and playing positions. This study also aimed to establish the optimal distance for the assessment of sprint speed and to compare the differences in anthropometrics, sprint and sprint momentum according to the age and playing position. Three hundred amateur rugby players performed anthropometric and physical fitness tests (10, 20, 30, 40 and 50 m sprint times, acceleration, velocity, and sprint momentum) during the in-season period. Participants from different age categories (under 14’s (U14), under 16’s (U16), under 18’s (U18) and Seniors) and positions (forwards and backs) volunteered to participate in this study. Results revealed that most of the U14 and U16 players (58.2% and 55.3%, respectively) reached maximum speed in the interval between 20 and 30 m with lower sprint speed than U18 players and Seniors (44% and 49%, respectively). Comparisons between each interval showed significant differences for all U14 and U16 forwards, suggesting the fastest interval was between 20-30 m. No significant differences were found for U16 backs, U18 and Seniors, between sprint times in the 20-30 m and 3040 m intervals. In addition, between-group comparisons, demonstrated significant (p < 0.001) differences in U14 when compared to U16, U18 and Seniors in anthropometric variables, sprint times and sprint momentum. In conclusion, this study suggests that the optimal distance for the assessment of sprint speed of rugby players is 30 m and that body mass, sprint momentum and sprint speed clearly discriminate between players of different age categories and playing positions.