1. A radioimmunoassay with monoclonal antibodies for the detection of antigenic cell-free Fc receptor
- Author
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Zahava Dux, Isaac P. Witz, Maya Ran, Nechama I. Smorodinsky, and Romema Anavi
- Subjects
Cell Extracts ,Antigenicity ,medicine.drug_class ,Immunology ,Fc receptor ,Radioimmunoassay ,chemical and pharmacologic phenomena ,Receptors, Fc ,Monoclonal antibody ,Cell Line ,Acetone ,chemistry.chemical_compound ,Epitopes ,Mice ,Antigen ,medicine ,Immunology and Allergy ,Animals ,Humans ,Lymphocytes ,Mice, Inbred BALB C ,biology ,Chemistry ,Antibodies, Monoclonal ,Molecular biology ,Monoclonal ,biology.protein ,Binding Sites, Antibody ,Antibody - Abstract
Making use of 125 I-labelled monoclonal rat antibodies against mouse Fc receptor we have developed a solid-phase radioimmunoassay for cell-free antigenic mouse Fc receptor (FcR). An 80% acetone solution was used for fixation of relatively large amounts of soluble proteins on PVC microtiter plates. As a result of this treatment FcR practically lost its activity to bind the Fc portion of an IgG molecule but retained its antigenicity, the binding of specific anti-FcR (aFcR) antibody being increased following acetone fixation. Concentrations of cell-free FcR in NP-40 extracts of FcR-expressing cells were calculated from the linear part of a standard curve and expressed in units of antigenic activity, 1 unit being the amount of antigenic FcR capable of binding 1 μg of 125 I-aFcR. The method may be used for detecting cell-free FcR as a minor constituent in a mixture of proteins.
- Published
- 1984