1. The BZLF1 homolog of an Epstein-Barr-related gamma-herpesvirus is a frequent target of the CTL response in persistently infected rhesus macaques
- Author
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Amitinder Kaur, Amany Awad, Mark H. Fogg, Deirdre Garry, and Fred Wang
- Subjects
Immunology ,Molecular Sequence Data ,Epitopes, T-Lymphocyte ,chemical and pharmacologic phenomena ,Biology ,Epitope ,Lymphocryptovirus ,Viral Proteins ,Immune system ,MHC class I ,Immunology and Allergy ,Animals ,Amino Acid Sequence ,Cells, Cultured ,ELISPOT ,Histocompatibility Antigens Class I ,Herpesviridae Infections ,Rhesus lymphocryptovirus ,biology.organism_classification ,Virology ,Macaca mulatta ,BZLF1 ,DNA-Binding Proteins ,CTL ,Tumor Virus Infections ,Haplotypes ,biology.protein ,Trans-Activators ,CD8 ,T-Lymphocytes, Cytotoxic - Abstract
Although CD8+ T lymphocytes targeting lytic infection proteins dominate the immune response to acute and persistent EBV infection, their role in immune control of EBV replication is not known. Rhesus lymphocryptovirus (rhLCV) is a γ-herpesvirus closely related to EBV, which establishes persistent infection in rhesus macaques. In this study, we investigated cellular immune responses to the rhLCV BZLF1 (rhBZLF1) homolog in a cohort of rhLCV-seropositive rhesus macaques. rhBZLF1-specific IFN-γ ELISPOT responses ranging between 56 and 3070 spot-forming cells/106 PBMC were detected in 36 of 57 (63%) rhesus macaques and were largely mediated by CD8+ T lymphocytes. The prevalence and magnitude of ELISPOT responses were greater in adult (5–15 years of age) rather than juvenile macaques (
- Published
- 2006