1. Characterization of the human Ig V lambda II gene family and analysis of V lambda II and C lambda polymorphism in systemic lupus erythematosus.
- Author
-
Paul E, Livneh A, Manheimer-Lory AJ, and Diamond B
- Subjects
- Alleles, Base Sequence, Cloning, Molecular, Haplotypes, Humans, Lupus Erythematosus, Systemic genetics, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, Genes, Immunoglobulin, Immunoglobulin Constant Regions genetics, Immunoglobulin Variable Region genetics, Immunoglobulin lambda-Chains genetics, Lupus Erythematosus, Systemic immunology, Polymorphism, Genetic
- Abstract
We report the cDNA sequence of an expressed human V lambda II gene and present an RFLP analysis of the Ig gene family defined by this clone. This V lambda II gene was expressed in a monoclonal B cell line generated from a patient with SLE by transformation with EBV. The encoded lambda L chain displays the 8.12 Id, an Id common to anti-DNA antibodies from patients with SLE. Using a coding region probe we estimate from Southern blot analysis that the germline V lambda II gene family contains at least 15 members. Many of the V lambda II restriction fragments are polymorphic both in SLE patients and in nonautoimmune individuals. EcoRI, HindIII, and TaqI RFLP analyses of the V lambda II gene family and EcoRI analysis of the C lambda gene family reveal no polymorphisms specific to SLE. Observed V lambda II and C lambda allele frequencies are the same among SLE patients and nonautoimmune individuals, and show no evidence of linkage disequilibrium between the two loci.
- Published
- 1991