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Start Over Author "Zuyong He" Journal journal of immunology research
2 results on '"Zuyong He"'

1. Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR

Author

Shuqi Xiao, Yaosheng Chen, Liangliang Wang, Jintao Gao, Delin Mo, Zuyong He, and Xiaohong Liu

Subjects
Immunologic diseases. Allergy, RC581-607
Abstract

Porcine reproductive and respiratory syndrome (PRRS) is a leading disease in pig industry worldwide and can result in serious economic losses each year. The PRRS epidemic situation in China has been very complicated since the unprecedented large-scale highly pathogenic PRRS (HP-PRRS) outbreaks in 2006. And now the HP-PRRS virus (HP-PRRSV) and classical North American type PRRSV strains have coexisted in China. Rapid differential detection of the two strains of PRRSV is very important for effective PRRS control. The real-time RT-PCR for simultaneous detection and differentiation of HP-PRRSV and PRRSV by using both SYBR Green and TaqMan probes was developed and validated. Both assays can be used for rapid detection and strain-specific identification of HP-PRRSV and PRRSV. However, the TaqMan probe method had the highest detection rate whereas the conventional RT-PCR was the lowest. The real-time RT-PCR developed based on SYBR Green and TaqMan probe could be used for simultaneous detection and differentiation of HP-PRRSV and PRRSV in China, which will benefit much the PRRS control and research.

Published
2014
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2. Simultaneous detection and differentiation of highly virulent and classical Chinese-type isolation of PRRSV by real-time RT-PCR

Author

Delin Mo, Xiaohong Liu, Jintao Gao, Yaosheng Chen, Liangliang Wang, Zuyong He, and Shuqi Xiao

Subjects
lcsh:Immunologic diseases. Allergy, Article Subject, Swine, Highly pathogenic, viruses, animal diseases, Immunology, Porcine Reproductive and Respiratory Syndrome, Virulence, Biology, Viral Nonstructural Proteins, Real-Time Polymerase Chain Reaction, Rapid detection, Sensitivity and Specificity, Virus, TaqMan, Immunology and Allergy, Animals, Porcine respiratory and reproductive syndrome virus, Outbreak, virus diseases, Reproducibility of Results, General Medicine, respiratory system, Isolation (microbiology), Virology, Real-time polymerase chain reaction, lcsh:RC581-607, Research Article
Abstract

Porcine reproductive and respiratory syndrome (PRRS) is a leading disease in pig industry worldwide and can result in serious economic losses each year. The PRRS epidemic situation in China has been very complicated since the unprecedented large-scale highly pathogenic PRRS (HP-PRRS) outbreaks in 2006. And now the HP-PRRS virus (HP-PRRSV) and classical North American type PRRSV strains have coexisted in China. Rapid differential detection of the two strains of PRRSV is very important for effective PRRS control. The real-time RT-PCR for simultaneous detection and differentiation of HP-PRRSV and PRRSV by using both SYBR Green and TaqMan probes was developed and validated. Both assays can be used for rapid detection and strain-specific identification of HP-PRRSV and PRRSV. However, the TaqMan probe method had the highest detection rate whereas the conventional RT-PCR was the lowest. The real-time RT-PCR developed based on SYBR Green and TaqMan probe could be used for simultaneous detection and differentiation of HP-PRRSV and PRRSV in China, which will benefit much the PRRS control and research.

Published
2013

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