1. Bactericidal/Permeability-Increasing Protein Release in Whole Blood Ex Vivo: Strong Induction by Lipopolysaccharide and Tumor Necrosis Factor
- Author
-
Anton T.J. Tool, Gaby J. M. Francot, Peter Vandenabeele, W. A. Buurman, Arthur J. Verhoeven, Pieter S. Hiemstra, Mieke A. Dentener, Pulmonologie, Algemene Heelkunde, and RS: NUTRIM School of Nutrition and Translational Research in Metabolism
- Subjects
Lipopolysaccharides ,Blood Bactericidal Activity ,medicine.medical_specialty ,Neutrophils ,Complement C5a ,Pharmacology ,Neutrophil Activation ,Receptors, Tumor Necrosis Factor ,chemistry.chemical_compound ,Azurophilic granule ,Antigens, CD ,Internal medicine ,medicine ,Humans ,Immunology and Allergy ,Platelet Activating Factor ,biology ,Platelet-activating factor ,Tumor Necrosis Factor-alpha ,Interleukin-8 ,Zymosan ,Membrane Proteins ,Blood Proteins ,N-Formylmethionine leucyl-phenylalanine ,Bactericidal/permeability-increasing protein ,Recombinant Proteins ,humanities ,N-Formylmethionine Leucyl-Phenylalanine ,Kinetics ,Infectious Diseases ,Endocrinology ,chemistry ,Receptors, Tumor Necrosis Factor, Type I ,biology.protein ,Tumor necrosis factor alpha ,Lipoteichoic acid ,Leukocyte Elastase ,Ex vivo ,Antimicrobial Cationic Peptides ,Interleukin-1 - Abstract
Department of Pulmonology, University of Limburg, Maastricht, Netherlands.In this study, the release of bactericidal/permeability-increasing protein (BPI), which is stored in polymorphonuclear leukocytes (PMNL), was analyzed in a whole blood ex vivo system. Of the microbial products tested, lipopolysaccharide (LPS) most potently induced BPI release; FMLP, serum-treated zymosan (STZ), and lipoteichoic acid (LTA) also induced BPI release. In addition, the inflammatory mediator tumor necrosis factor (TNF)-alpha potently activated PMNL in whole blood, via TNF receptor p55, to release BPI, whereas interleukin (IL)-1, IL-8, platelet activating factor, and C5a were poor inducers of BPI release. STZ and phorbol myristate acetate, but not LPS, FMLP, or LTA, stimulated isolated PMNL to release BPI. BPI was released in comparable magnitude with the azurophilic granule protein elastase. Furthermore, both proteins were released with similar kinetics, which started within 30 min after onset of stimulation and lasted 1-4 h.
- Published
- 1997