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Your search keyword '"Zhu, Meifei"' showing total 3 results
Start Over Author "Zhu, Meifei" Journal journal of international medical research
3 results on '"Zhu, Meifei"'

2. Total flavonoids from Astragalusalleviate endothelial dysfunction by activating the Akt/eNOS pathway

Author

Liu, Qian, Zhang, Ling, Shan, Qiyuan, Ding, Yuxia, Zhang, Zhaocai, Zhu, Meifei, and Mao, Yuanjie

Abstract

Objective To investigate the vasodilative and endothelial-protective effects and the underlying mechanisms of total flavonoids from Astragalus(TFA).Methods The vasodilative activities of TFA were measured with a myograph ex vivousing rat superior mesenteric arterial rings. The primary human umbilical vein endothelial cell (HUVEC) viabilities were assayed using the cell counting kit-8 after hypoxia or normoxia treatment with or without TFA. Akt, P-Akt, eNOS, P-eNOS, Erk, P-Erk, Bcl-2 and Bax expression were analyzed using western blotting.Results TFA showed concentration-dependent vasodilative effects on rat superior mesenteric arterial rings, but had no effects on normal or potassium chloride precontracted arterial rings. TFA did not affect HUVEC viabilities in normoxia, but dramatically promoted cell proliferation in the concentration range of 1 to 30 µg/mL under hypoxia. Moreover, TFA significantly increased the ratios of P-Akt/Akt and P-eNOS/eNOS in vascular endothelial cells under hypoxic conditions, but did not change the P-Erk/Erk or Bcl-2/Bax ratios.Conclusions TFA might exhibit vasorelaxant and endothelial-protective effects via the Akt/eNOS signaling pathway.

Published
2018
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3. Total flavonoids from Astragalus alleviate endothelial dysfunction by activating the Akt/eNOS pathway.

Author

Liu Q, Zhang L, Shan Q, Ding Y, Zhang Z, Zhu M, and Mao Y

Subjects
Animals, Apoptosis drug effects, Cell Survival drug effects, Human Umbilical Vein Endothelial Cells drug effects, Humans, Hypoxia physiopathology, Male, Mesenteric Artery, Superior drug effects, Models, Animal, Nitric Oxide Synthase Type III metabolism, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt metabolism, Rats, Rats, Sprague-Dawley, Signal Transduction, Astragalus Plant, Drugs, Chinese Herbal therapeutic use, Endothelial Cells drug effects, Flavonoids pharmacology, Vasodilation drug effects
Abstract

Objective To investigate the vasodilative and endothelial-protective effects and the underlying mechanisms of total flavonoids from Astragalus (TFA). Methods The vasodilative activities of TFA were measured with a myograph ex vivo using rat superior mesenteric arterial rings. The primary human umbilical vein endothelial cell (HUVEC) viabilities were assayed using the cell counting kit-8 after hypoxia or normoxia treatment with or without TFA. Akt, P-Akt, eNOS, P-eNOS, Erk, P-Erk, Bcl-2 and Bax expression were analyzed using western blotting. Results TFA showed concentration-dependent vasodilative effects on rat superior mesenteric arterial rings, but had no effects on normal or potassium chloride precontracted arterial rings. TFA did not affect HUVEC viabilities in normoxia, but dramatically promoted cell proliferation in the concentration range of 1 to 30 µg/mL under hypoxia. Moreover, TFA significantly increased the ratios of P-Akt/Akt and P-eNOS/eNOS in vascular endothelial cells under hypoxic conditions, but did not change the P-Erk/Erk or Bcl-2/Bax ratios. Conclusions TFA might exhibit vasorelaxant and endothelial-protective effects via the Akt/eNOS signaling pathway.

Published
2018
Full Text
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