1. Treatment of Experimental Subcutaneous Human Melanoma with a Replication-Restricted Herpes Simplex Virus Mutant.
- Author
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Randazzo, Bruce P., Bhat, Mulki G., Kesari, Santosh, Fraser, Nigel W., and Brown, S. Moira
- Subjects
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MELANOMA , *HERPES simplex virus , *BRAIN tumors , *PROTEINS , *IMMUNOHISTOCHEMISTRY , *NEUROENDOCRINE tumors - Abstract
Modified, non-neurovirulent herpes simplex viruses (HSV) have shown promise for the treatment of brain tumors, including intracranial melanoma. In this report, we show that HSV-1716, an HSV-1 mutant lacking both copies of the gene coding-infected cell protein 34.5 (ICP 34.5), can effectively treat experimental subcutaneous human melanoma in mice. In vitro, HSV-1716 replicated in all 26 human melanoma cell lines tested, efficiently lysing the cells. Therapeutic infection of subcutaneous human melanoma nodules with HSV-.171.6 led to viral replication that was restricted to tumor cells by immunohistochemistry. Moreover, HSV-1716 treatment significantly inhibited progression of preformed subcutaneous human melanoma nodules in SCID mice and caused complete regression of some tumors. This work expands the potential scope of HSV-1-based cancer therapy. [ABSTRACT FROM AUTHOR]
- Published
- 1997
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