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Background: Utilizing pollinators is one of the most dependable, economical, and environmentally friendly ways to improve cross-pollination, boost crop output, and improve crop quality. Bees are regarded as the best pollinators among all other pollinators. Methods: The current study was conducted to elucidate the impact of honeybee (Apis mellifera) visits on the pollination of various sunflower cultivars including Hysum-33, Hysun-38, and FH-106. Results: Our finding revealed that the highest average number of honeybee visits was observed in the morning followed by noon and afternoon on different sunflower cultivars. The maximum number of honeybee visits was recorded on Hysun-38, while the minimum was recorded on the FH-106 cultivar, which was 87.85 and 73.6, respectively. In (Hysun-33) and (FH-106), the maximum average time spent by the honeybee noticed in the morning on the flower was 34.24 and 32.67 min, whereas the maximum average time spent in the evening on the flower of (Hysun-38), which was 36.41 min. Regression analysis demonstrated that a significant effect between the head diameter of sunflower cultivars and the number of honey bee visits (p

The most important honey bee queen food royal jelly is produced by the exocrine hypopharyngeal glands (HGs) of the worker honey bees. The HGs exhibits diverse gene and protein that create age-related physiological adaptations. However, limited knowledge is available on how the phosphorylation process is responsible for physiological alterations across the development of HGs in newly emerged bees. This study measured the acinus of HGs and characterized its phosphoproteomics analysis between the newly emerged bees of royal jelly bees (RJBs) and Italian bees (ITBs). Phosphopeptides of HGs were enriched by Ti4+-IMAC reagents, followed by protein identification via Q-Exactive LC-MS/MS. Our findings indicated that the mean acinus size of HGs of newly emerged bees of RJBs was significantly larger (56.18 ± 1.72 µm) than ITBs (45.98 ± 1.62 µm). A total of 1576 phosphopeptides with 1800 phosphosites containing 525 phosphoproteins were identified in RJBs, while 746 phosphopeptides, of which 846 phosphosites correspond to 317 phosphoproteins were identified in ITBs. Most proteins were phosphorylated on 1 residue followed by 2 and 3 residues in newly emerged bees of both bee stocks. In addition, serine phosphorylation was most observed, followed by threonine and tyrosine in both bee stocks. In newly emerged bees of RJBs, the protein metabolic process, glycolytic process, and formation of translation preinitiation were uniquely enriched, while protein translation, peptide metabolic process and elongation were enriched in ITBs. This research shows detailed phosphorylation of HGs and provides helpful information for understanding the biological activities of HGs development in newly emerged bees from both bee stocks.

Honey bee queen serves as a colony's centre hub, producing eggs and releasing pheromones to keep a colony together. Artificial insemination in Apis mellifera, trending research these days, is a consistent method for controlling mating in honey bees. Quality queen production is essential to maintaining a healthy bee colony. A review of comparative studies on superior quality honey bee (A. mellifera) queens production through nontraditional techniques and various factors affecting the instrumental insemination process has been compiled in this article. Several factors like; rearing conditions, stress, inseminator’s skills, food availability, mating age of queen, queen banking, temperature, sperms stored in the spermatheca, sperms quality and quantity, semen handling and storage, CO2 and nitrogen treatments, pheromones affect the performance of instrumentally inseminated queens. Moreover, different data collection showed that different methods used for queen treatment which have a significant effect on the performance of the queen. This review suggested that artificially inseminated queens could be more productive if experiments were done with proper attention and precision.

Artificial queen rearing has changed the beekeeping business in contrast to natural queen replacement, as it provides a newly mated queen into a bee colony, reduces the time between eggs not being laid, and increases the production of young bees in a colony. This study was conducted to investigate the larval acceptance rate grafted in queen cups made from different materials and to find out whether the various materials used for queen cups were suitable for the acceptance of grafted larvae in cell builder colonies. The evaluated materials included fresh bee wax (T1), old bee wax (T2), uncapping bee wax (T3), pure paraffin (T4), 50% paraffin + 50% old bee wax (T5), and brown plastic queen cups (T6) as a control. Results indicated that T3 was the material that most increased the larval acceptance (5.2 ± 0.37), followed by T4 (2.4 ± 0.5), T2 (2.2 ± 0.58), T5 (1.8 ± 0.37), T1 (1.6 ± 0.4), and minimum larval acceptance was in the T6 (1.0 ± 0.4) respectively during the spring season of the year 2020–2021. Similar findings were reported of larval acceptance rate during the spring season of 2021–2022. In addition, the wax material that least affected larval acceptance was the fresh comb bee wax. However, all materials used were coupled with a more larval acceptance rate than the control treatment during both spring seasons of 2020–2021 and 2021–2022. The larval acceptance rate was statistically significant in T3 as a compared to other queen cell cup materials during the both spring seasons (p = 0.001). These findings imply that using different types of bee wax for preparing queen cell cups during larvae grafting, particularly uncapping bee wax, may stimulate and promote grafted larvae acceptance during the queen rearing process.

To determine the effect of honey bee (Apis mellifera L.) pollination on sunflower yield using the most appropriate number of beehives per unit area of crops is very important. By comparing the number of hives and yield components, we can satisfy optimal pollination needs and improve economic yields. For this purpose, a series of experiments were conducted at thatha Khalil farmer field Taxila Rawalpindi, under the supervision of Beekeeping and Hill Fruit Pests Research, Station Rawalpindi, Pakistan, with four treatments for comparison: 1 hive acre−1, 2 hives acre−1, 3 hives acre−1, and 0 hives acre−1 as a control. The hives were kept inside the experimental area and pollination density, pollinator’s diversity, agronomic yield, and economic yield were determined. Two acres of sunflower field were sown and four bee colonies were introduced at 5% to 10% flowering. The maximum visitation frequency of A. mellifera was recorded at the weekly interval from 24 to 09-2019 to 24–10-2019, according to which maximum visitation was observed (44.8, 38.4, and 16.5 plant−1 5 min−1) at 10:00, 12:00, and 14:00 hrs, respectively. Data regarding foraging pollinators revealed that A. mellifera was the most dominant pollinator of the sunflower crop with the highest abundance 17.11% followed by A. cerana (3.46%) and the population of A. dorsata was minimum (2.12%). Furthermore, agronomic parameters (number of seeds plant−1, 100 seed weight (g), and economic yield were significantly higher in those fields which were pollinated by more beehives in comparison to fewer beehives.