1. Identification of mature and immature human thymic dendritic cells that differentially express HLA-DR and interleukin-3 receptor in vivo
- Author
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Jean Claude Gluckman, Marie-José Alpha, Christian Schmitt, Hélène Fohrer, A H Dalloul, Sylvie Beaudet, Pierre Palmer, Bruno Canque, laboratoire d'immunologie cellulaire et tissulaire (UMR7627), Centre National de la Recherche Scientifique (CNRS), School of Geosciences [Edinburgh], University of Edinburgh, CHU Pitié-Salpêtrière [APHP], and École pratique des hautes études (EPHE)
- Subjects
Antigens, Differentiation, T-Lymphocyte ,Myeloid ,medicine.medical_treatment ,[SDV]Life Sciences [q-bio] ,MESH: Membrane Glycoproteins ,CD34 ,MESH: Antigens, Neoplasm ,Antigens, CD34 ,[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity ,MESH: Cadherins ,MESH: Hematopoietic Stem Cells ,0302 clinical medicine ,MESH: Receptors, Granulocyte-Macrophage Colony-Stimulating Factor ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,MESH: Vesicular stomatitis Indiana virus ,Immunology and Allergy ,Myeloid Cells ,MESH: Antigens, CD ,Cells, Cultured ,0303 health sciences ,Lymphokines ,Membrane Glycoproteins ,MESH: Dendritic Cells ,Reverse Transcriptase Polymerase Chain Reaction ,MESH: Infant, Newborn ,Cell Differentiation ,Cadherins ,Fetal Blood ,MESH: Gene Expression Regulation ,Cytokine ,medicine.anatomical_structure ,[SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology ,Receptors, Granulocyte-Macrophage Colony-Stimulating Factor ,MESH: Interferon-alpha ,MESH: Cells, Cultured ,MESH: Cell Differentiation ,MESH: Interleukins ,MESH: Immunophenotyping ,MESH: Interferon-gamma ,CD14 ,Immunology ,Interleukin-3 Receptor alpha Subunit ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Thymus Gland ,Biology ,MESH: Receptors, Interleukin-3 ,Respirovirus ,Vesicular stomatitis Indiana virus ,Immunophenotyping ,03 medical and health sciences ,Interferon-gamma ,Th2 Cells ,Antigen ,MESH: Th2 Cells ,Antigens, CD ,Antigens, Neoplasm ,medicine ,HLA-DR ,Humans ,MESH: Fetal Blood ,Cell Lineage ,030304 developmental biology ,MESH: Interleukin-3 Receptor alpha Subunit ,CD40 ,MESH: Humans ,MESH: Lymphokines ,Interleukins ,Infant, Newborn ,Interferon-alpha ,Cell Biology ,MESH: Antigens, CD34 ,MESH: Thymus Gland ,Dendritic Cells ,HLA-DR Antigens ,MESH: Cell Lineage ,MESH: HLA-DR Antigens ,Hematopoietic Stem Cells ,Molecular biology ,MESH: Myeloid Cells ,Receptors, Interleukin-3 ,MESH: Respirovirus ,MESH: Antigens, Differentiation, T-Lymphocyte ,Gene Expression Regulation ,biology.protein ,MESH: Biomarkers ,Interleukin-3 receptor ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Biomarkers ,030215 immunology - Abstract
We have previously shown that thymic CD34 1 cells have a very limited myeloid differentia- tion capacity and differentiate in vitro mostly into CD1a 1 -derived but not CD14 1 -derived dendritic cells (DC). Herein we characterized the human neo- natal thymic DC extracted from the organ in rela- tionship with the DC generated from CD34 1 cells in situ. We show that in vivo thymic DC express E cad- herin, CLA, CD4, CD38, CD40, CD44, and granu- locyte-macrophage colony-stimulating factor-R (GM- CSF-R; CD116) but no CD1a. According to their morphology, functions, and surface staining they could be separated into two distinct subpopulations: mature HLA-DR hi , mostly interleukin-3-R (CD123)- negative cells, associated with thymocytes, some apoptotic, and expressed myeloid and activation markers but no lymphoid markers. In contrast, immature HLA-DR 1 CD123 hi CD36 1 cells with monocytoid morphology lacked activation and my- eloid antigens but expressed lymphoid antigens. The latter express pTa mRNA, which is also found in CD34 1 thymocytes and in blood CD123 hi DC further linking this subset to lymphoid DC. How- ever, the DC generated from CD34 1 thymic pro- genitors under standard conditions were pTa-neg- ative. Thymic lymphoid DC showed similar pheno- type and cytokine production profile as blood/ tonsillar lymphoid DC but responded to GM-CSF, and at variance with them produced no or little type I interferon upon infection with viruses and did not induce a strict polarization of naive T cells into TH2 cells. Their function in the thymus re- mains therefore to be elucidated. J. Leukoc. Biol. 68: 836-844; 2000.
- Published
- 2000
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