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Candida albicansbelongs to our commensal mucosal flora and in immune-competent individuals in the absence of epithelial damage, this fungus is well tolerated and controlled by our immune defense. However, C. albicansis an opportunistic microorganism that can cause different forms of infections, ranging from superficial to life-threatening systemic infections. C. albicansis polymorphic and switches between different phenotypes (e.g. from yeast form to hyphal form). C. albicanshyphae are invasive and can grow into tissues to eventually reach circulation. During fungal infections, neutrophils in particular play a critical role for the defense, but how neutrophils are directed toward the invasive forms of fungi is less well understood. We set out to investigate possible neutrophil chemoattractants released by C. albicansinto culture supernatants. We found that cell-free culture supernatants from the hyphal form of C. albicansinduced both neutrophil chemotaxis and concomitant intracellular calcium transients. Size separation and hydrophobic sorting of supernatants indicated small hydrophilic factors as responsible for the activity. Further analysis showed that the culture supernatants contained high levels of short-chain fatty acids with higher levels from hyphae as compared to yeast. Short-chain fatty acids are known neutrophil chemoattractants acting via the neutrophil free fatty acid receptor 2. In line with this, the calcium signaling in neutrophils induced by hyphae culture supernatants was blocked by a free fatty acid receptor 2 antagonist and potently increased in the presence of a positive allosteric modulator. Our data imply that short-chain fatty acids may act as a recruitment signal whereby neutrophils can detect C. albicanshyphae.During hyphal growth, Candida albicansrelease high levels of short-chain fatty acids which can function as end-point chemoattractants directing neutrophils toward invasive growth forms of C. albicans.