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The study aimed to establish the detection method of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method. The separation was performed by using acetonitrile -0.1% formic acid solution as mobile phase and 5% ammoniated methanol as eluent. The detection was conducted by using HPLC-ESI-MS/MS in positive ion and multiple reaction monitoring (MRM) modes. The results showed that this method possessed satisfactory detecting rate for clenbuterol ranging from 0.050 ng/mL to 5.000 ng/mL. The coefficient was 0.997 8, the recovery rate was 98% ~ 102%, and the relative standard deviation was 2.09%. With high sensitivity and accuracy, this method could be used for detecting the clenbuterol in lamb, and it also had certain guiding significance for quantitative analysis of clenbuterol in other meat products. [ABSTRACT FROM AUTHOR]