1. Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma
- Author
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Cian Monnin, Anthony D. Postle, S. J. Kumari A. Ubhayasekera, Matej Orešič, Tomas Cajka, Jacquelyn M. Weir, Candice Z. Ulmer, Stephen E. Somerville, Xueqing Zhao, Therese Koal, Renu Nandakumar, Senlin Zhou, Denis Reynaud, John A. Bowden, James E. Evans, Joost Brandsma, Rhishikesh Thakare, Jeremy P. Koelmel, Houli Jiang, Tuulia Hyötyläinen, Christoph H. Borchers, Oliver Fiehn, J. Will Thompson, Susanne Sales, Karen Lin, Christina M. Jones, Jun Han, Aveline H. Neo, Laila Abdullah, Charles N. Serhan, Mary R. Roth, Danielle J. McDougall, Alexander Triebl, Martin Trötzmüller, Yazen Alnouti, Serge Cremers, Michelle Cinel, Irwin J. Kurland, Kai Schuhmann, Craig E. Wheelock, Min Yuan, Romain A. Colas, Ruth Welti, Yingying Huang, Hiroaki Takeda, Timothy J. Garrett, Jon Rees, Takeshi Bamba, Grielof Koster, Michal A. Surma, Libin Yao, Natalie A. Mellett, Johan Kolmert, M. Arthur Moseley, Krishna Rao Maddipati, Harald Köfeler, John M. Asara, Dajana Vuckovic, Aaron M. Armando, Michael S. Gardner, Peter J. Meikle, Rebecca S. Pugh, Yoshihiro Izumi, Alexander Fauland, Antonio Checa, Jonas Bergquist, Amaury Cazenave-Gassiot, Parsram Ramrup, Zsuzsanna Kuklenyik, Alan Heckert, Hongfeng Jiang, Yunping Qiu, David A. Peake, Oswald Quehenberger, Markus R. Wenk, John R. Barr, Michael Leadley, Linda Ahonen, Barbara Rembiesa, Jiang Jiang, Martin Post, Kristaps Klavins, Susanne B. Breitkopf, Lisa St. John-Williams, Michal L. Schwartzman, Edward A. Dennis, Andrej Shevchenko, Katherine H. Gotlinger, Federico Torta, Christian Klose, Jason S. Pierce, Rainey E. Patterson, Reiko Kiyonami, and Maureen Kachman
- Subjects
0301 basic medicine ,Biochemistry & Molecular Biology ,Laboratory Proficiency Testing ,International Cooperation ,sterols ,QD415-436 ,Medical Biochemistry and Metabolomics ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,Endocrinology ,fatty acyls ,Lipidomics ,Humans ,Ionization mass spectrometry ,quality control ,Reference standards ,phospholipids ,Research Articles ,Observer Variation ,sphingolipids ,Chromatography ,quantitation ,Chemistry ,010401 analytical chemistry ,Standard Reference Material 1950 ,glycerolipids ,ta1182 ,Reproducibility of Results ,Cell Biology ,National Institute of Standards and Technology ,Reference Standards ,Lipid Metabolism ,Lipids ,0104 chemical sciences ,Cell and molecular biology ,Benchmarking ,030104 developmental biology ,Human plasma ,NIST ,Biochemistry and Cell Biology ,Targeted metabolomics - Abstract
As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.
- Published
- 2017