1. Sequential protein NMR assignments in the liquid state via sequential data acquisition
- Author
-
Christian Herbst, Matthias Görlach, Sabine Häfner, Christoph Wiedemann, Ramadurai Ramachandran, Peter Bellstedt, and Anika Kirschstein
- Subjects
Carbon Isotopes ,Nuclear and High Energy Physics ,Materials science ,Nitrogen Isotopes ,ved/biology ,Sulfolobus solfataricus ,ved/biology.organism_classification_rank.species ,Biophysics ,Proteins ,Resonance ,Chromosomes, Bacterial ,Winged Helix ,Condensed Matter Physics ,Biochemistry ,Spectral line ,Crystallography ,Electromagnetic Fields ,Liquid state ,Data acquisition ,Minichromosome maintenance ,Peptide bond ,Indicators and Reagents ,Nuclear Magnetic Resonance, Biomolecular - Abstract
Two different NMR pulse schemes involving sequential 1 H data acquisition are presented for achieving protein backbone sequential resonance assignments: (i) acquisition of 3D {HCCNH and HNCACONH} and (ii) collection of 3D {HNCOCANH and HNCACONH} chemical shift correlation spectra using uniformly 13 C, 15 N labelled proteins. The sequential acquisition of these spectra reduces the overall experimental time by a factor of ≈ 2 as compared to individual acquisitions. The suitability of this approach is experimentally demonstrated for the C-terminal winged helix (WH) domain of the minichromosome maintenance (MCM) complex of Sulfolobus solfataricus .
- Published
- 2014