1. Antioxidant and multi-sensitive PNIPAAm/keratin double network gels for self-stripping wound dressing application
- Author
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Xiuzhen Wan, Jian Shen, Bo Chi, Jie Dou, Jiang Yuan, Rong Yang, and Xiao Han
- Subjects
Antioxidant ,Morphology (linguistics) ,Stripping (chemistry) ,medicine.medical_treatment ,Acrylic Resins ,Biomedical Engineering ,Biocompatible Materials ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Antioxidants ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Picrates ,Materials Testing ,Keratin ,medicine ,Animals ,General Materials Science ,chemistry.chemical_classification ,Wound Healing ,integumentary system ,Biphenyl Compounds ,Hydrogels ,General Chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,Bandages ,Anti-Bacterial Agents ,Rats ,0104 chemical sciences ,chemistry ,Covalent bond ,Acrylamide ,Biophysics ,Keratins ,Swelling ,medicine.symptom ,0210 nano-technology ,Antibacterial activity - Abstract
Hydrogel is a potential wound dressing material due to its ability to maintain a humid environment, the strong absorptive capacity of exuded tissue fluid, and gas exchange function. Herein, poly(N-isopropyl acrylamide)/keratin double network (PNIPAAm/keratin DN) gels were fabricated through covalent and ionic double cross-linking strategy. The effects of PNIPAAm/keratin ratios on the morphology and swelling rate of gels were characterized. The DN gels could swell up from 2600% to 4600% in proportion to the keratin content, demonstrating their great ability to absorb tissue fluid. The gels possessed thermo-sensitiveness, imparting self-stripping property. Moreover, the antibacterial chlorhexidine acetate (CHX) was loaded into gels with a post-fabrication drug-loading strategy. The release behavior showed that CHX-loaded DN gels exhibited multiple responsive characteristics (temperature, pH, and ROS). Furthermore, the drug-loaded gels showed greater antibacterial activity than free CHX due to the sustained drug release effect. Meanwhile, the antioxidant efficiency of PNIPAAm/keratin DN gels was ca. 33.1%, while the PNIPAAm gel was just ca. 18.2%, indicating the strong oxidation resistance of DN gels. In the Sprague Dawley (SD) rat skin defect model, the hydrogel had better tissue regeneration ability than the commercial film. Taken together, the multifunctional PNIPAAm/keratin DN gels are potential candidates for clinical wound treatment.
- Published
- 2021
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