1. A novel in vitro model for preclinical testing of the hemocompatibility of intravascular stents according to ISO 10993-4
- Author
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Gerhard Ziemer, Stefan Sinn, Stephan Deichelbohrer, Hans Peter Wendel, and Torsten Scheuermann
- Subjects
Blood Platelets ,Male ,medicine.medical_treatment ,Biomedical Engineering ,Biophysics ,Thrombogenicity ,Hemocompatibility Testing ,Bioengineering ,In Vitro Techniques ,Models, Biological ,Biomaterials ,Coated Materials, Biocompatible ,Blood vessel prosthesis ,Materials Testing ,medicine ,Humans ,Platelet activation ,Blood Coagulation ,Whole blood ,Inflammation ,Blood Cells ,Heparin ,business.industry ,Anticoagulants ,Stent ,Drug-Eluting Stents ,Reference Standards ,Platelet Activation ,equipment and supplies ,Blood Cell Count ,Blood Vessel Prosthesis ,medicine.anatomical_structure ,Microscopy, Electron, Scanning ,Stents ,business ,Biomedical engineering ,Artery ,medicine.drug - Abstract
Subacute stent thrombosis, caused by undesired interactions between blood and the stent surface, is a major concern in the first few weeks following coronary artery stent implantation. The aim of this study was to establish a novel in vitro model for hemocompatibility testing of coronary artery stents according to ISO 10993-4. The model consists of a modified Chandler-Loop design with closed heparin-coated PVC Loops and a thermostated water bath. The tests were performed with anticoagulated human whole blood. After incubation in the loop, blood was analyzed for coagulation and inflammatory activation markers (TAT, β-TG, sP-selectin, SC5b-9 and PMN-elastase). Three different stent types with varying thrombogenicity were tested; statistically significant differences were found between the three stent types in measures of coagulation and platelet activation. The new Chandler-Loop model can be used as an alternative to animal and current in vitro models, especially for the determination of early events after stent implantation.
- Published
- 2011
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