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Introduction: This study examined Vickers hardness as well as surface characteristics of different computer-aided design/computer-aided manufacturing (CAD/CAM) resin composites prior to and after storage in various media., Materials and Methods: CAD/CAM resin composite blocks (Grandio Blocs (GB), Lava Ultimate (LU), Brilliant Crios (BC), Cerasmart (GC), Shofu Block HC (SB), Tetric CAD (TC), Luxacam Composite (LC); incl. different translucency variants) were prepared, polished and surface free energy was determined. The specimens were divided into four groups: dry conditions for 24 h (25 °C), demineralized water (37 °C), Pepsi Cola (37 °C) and 75% ethanol (37 °C). After seven and 28 days of storage, Vickers hardness was determined. Surface roughness was measured after the entire storage period., Results and Discussion: Vickers hardness was in the range of about 150 HV for GB, around 115 HV for LU, and 80-100 HV for BC, GC, SB, TC and LC. Only minor differences (total: 50.2 (6.4)-56.2 (3.2) mN/m) in surface free energy could be detected. No relationship was observed between surface free energy and filler content. However, a correlation between filler content and Vickers hardness was evident. Artificial aging caused a decrease of Vickers hardness (up to -40 HV or 35%) depending on storage media, duration and material. The changes in surface texture after immersion in different media were below a value of ΔSa = 0.015 µm., Conclusion: Artificial aging of CAD/CAM resin composites leads to a significant decrease of Vickers hardness for most materials, while only small changes in surface roughness were identified., (© 2023. The Author(s).)

This study aimed at evaluating bacterial adhesion and biofilm formation on resin-based composites (RBC) including dicalcium phosphate dihydrate nanoparticles (nDCPD)., Methods: Specimens were prepared from experimental RBCs with BisGMA/TEGDMA resin matrix including 20 vol% of either nDCPD (nDCPD-RBC), TEGDMA-functionalized nDPCD (F-nDCPD-RBC) or silanized silica (SiO 2 -RBC). Neat resin blend (control-Resin), conventional nanohybrid RBC (control-RBC) and human enamel were used for reference. Characterization of the specimens included surface roughness (SR), surface free energy (SFE), chemical surface composition (EDS, XPS), and buffering ability of a pH = 4.00 solution. Streptococcus mutans adherence was assessed after 2 h; biofilm formation was simulated for 48 h using a bioreactor. Adherent, viable biomass was determined using tetrazolium salt assay (MTT)., Results: nDCPD-RBC yielded highest roughness and showed higher polar and lower disperse component to total SFE. EDS and XPS indicated higher amounts of calcium and phosphate on the surface of nDCPD-RBC than on F-nDCPD-RBC. nDCPD buffered the acidic solution to 5.74, while functionalization almost prevented buffering (pH = 4.26). F-nDCPD-RBC reduced adherence and biofilm formation in comparison to nDCPD-RBC. Regardless of functionalization, biofilm formation on nDCPD-containing RBCs was not significantly different from SiO 2 -RBC. Control-Resin, control-RBC, and enamel surfaces showed similar adherence values as F-nDCPD-RBC, but lower biofilm formation compared to both nDCPD-containing RBCs. In conclusion, the incorporation of nDCPD did not minimize S. mutans adherence and biofilm formation as a function of the materials´ surface properties. However, results observed for the buffering capacity indicated that optimized formulations of biomimetic RBCs may be useful for modulating their interaction with microorganisms.

The aim of this study was to investigate the impact of resin matrix chemistry and filler fraction on biofilm formation on the surface of experimental resin-based composites (RBCs). Specimens were prepared from eight experimental RBC formulations differing in resin matrix blend (BisGMA/TEGDMA in a 7:3 wt% ratio or UDMA/aliphatic dimethacrylate in a 1:1 wt% ratio) and filler fraction (no fillers; 65 wt% dental glass with an average diameter of 7 or 0.7 µm or 65 wt% SiO2 with an average diameter of 20 nm). Surface roughness, surface free energy, and chemical surface composition were determined; surface topography was visualized using atomic force microscopy. Biofilm formation was simulated under continuous flow conditions for a 48 h period using a monospecies Streptococcus mutans and a multispecies biofilm model. In the monospecies biofilm model, the impact of the filler fraction overruled the influence of the resin matrix, indicating lowest biofilm formation on RBCs with nano-scaled filler particles and those manufactured from the neat resin blends. The multispecies model suggested a more pronounced effect of the resin matrix blend, as significantly higher biofilm formation was identified on RBCs with a UDMA/dimethacrylate matrix blend than on those including a BisGMA/TEGDMA matrix blend but analogous filler fractions. Although significant differences in surface properties between the various materials were identified, correlations between the surface properties and biofilm formation were poor, which highlights the relevance of surface topography and chemistry. These results may help to tailor novel RBC formulations which feature reduced biofilm formation on their surface.

The aim of this in vitro study was to investigate the influence of artificial ageing on the surface properties and early Streptococcus mutans adhesion to current dental composites for the direct restoration of class II defects. Three hundred and thirty specimens each were prepared from five dental composites, and were randomly allotted to various artificial ageing protocols (storage in distilled water/ethanol/artificial saliva for 7/90/365 days; thermal cycling, 6,000 cycles 5/55 degrees C). Prior and after each treatment, surface roughness (R(a)) and hydrophobicity were determined, and S. mutans adhesion (ATCC 25175; 2.5 h, 37 degrees C) was simulated with and without prior exposition to human whole saliva (2 h, 37 degrees C). Adherence of S. mutans was determined fluorometrically. Means and standard deviations were calculated, and analyzed using three-way ANOVA and post-hoc analysis (alpha = 0.05). For both R(a) and S. mutans adherence to uncoated and saliva-coated specimens, significant influences of the composite material, the ageing medium and the ageing duration have been observed; for surface hydrophobicity, significant influences of the composite material and the ageing duration were found. For uncoated specimens, significant increases in S. mutans adhesion were observed with prolonged artificial ageing, whereas significant decreases in S. mutans adhesion were found for the saliva-coated specimens. The data indicate influences of the artificial ageing method on surface parameters such as R(a) and hydrophobicity as well as microbial adhesion. The results underline the relevance of saliva coating on the outcome of studies simulating microbial adhesion, and highlight differences in the susceptibility of dental composites for the adhesion of oral bacteria.

Objective: The aim of this in vitro study was to examine potential changes and influences of prosthetic resin surface properties on Candida albicans adhesion after surface treatment or artificial ageing., Materials and Methods: Standardized specimens of a denture base resin and a veneering composite were prepared, polished, and randomly subjected to different surface roughness treatments or artificial ageing protocols (storage in ethanol or artificial saliva for 7/90 d, thermocycling). Surface roughness (Ra) and surface free energy were determined prior and after each treatment. Specimens were incubated with phosphate buffered saline or whole saliva for 2 h at 37 degrees C, and later with Candida albicans suspension (2.5 h, 37 degrees C). Adherent viable fungi were quantified using a bioluminescence assay., Results: Artifical ageing did not affect substratum surface roughness, yet slight increases in substratum surface free energy and significant increases in Candida albicans adhesion were observed. Saliva coating marginally influenced Candida albicans adherence to reference and surface treated specimens, yet more pronounced differences in Candida albicans adhesion between the various artificially aged specimens were found., Conclusion: No correlation between substratum surface roughness or surface free energy and Candida albicans adhesion could be established.

Objectives: This study aimed to characterize the surface properties of experimental resin polymers consisting of monomers differing in functionality and chain length, and to evaluate differences in Streptococcus mutans adhesion., Material and Methods: Six resins were prepared (70/30 ratio UDMA/monomer); camphorquinone and ethyl-4-dimethylaminebenzoate were added for light activation. A conventional composite was used as a control. Surface free energy was determined prior and after saliva exposition (2 h, 37 degrees C). After saliva incubation (2 h, 37 degrees C), specimens were incubated with Streptococcus mutans NCTC 10449 for 2.5 h at 37 degrees C. Adherent bacteria were quantified by determining the relative substratum area covered by bacteria using SEM analysis, and by using a fluorometric assay for viable cell quantification., Results: No statistically significant differences in total surface free energies were found for uncoated specimens (mean total surface free energies ranging from 39.79 to 49.73 mJ/m(-2)); after saliva coating, statistically significant differences were observed for some of the polymers (mean total surface free energies ranging from 44.13 to 65.81 mJ/m(-2)). Few differences were observed between SEM and fluorescence quantification, finding statistically significant differences in streptococcal adhesion to the experimental polymers. Median bacteria surface coverage ranged from 1.4% for UDMA mixed with 1,10-decandiol dimethacrylate to 16.2% for the control composite material; lowest fluorescence intensities indicating lowest adhesion of bacteria were found for UDMA mixed with 1,10-decandiol dimethacrylate (median 712), and highest values indicating highest adhesion of bacteria were found for UDMA mixed with polyethyleneglycol (600) dimethacrylate (median 11974)., Conclusion: Streptococcus mutans adhesion appears to be different on polymers differing in monomer mixtures, yet correlations between substratum surface free energy and streptococcal adhesion were poor. Further studies are necessary to evaluate additional substratum surface properties and pellicle distribution and composition more thoroughly.