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Your search keyword '"DNA, Protozoan cerebrospinal fluid"' showing total 2 results
Start Over Descriptor "DNA, Protozoan cerebrospinal fluid" Journal journal of medical microbiology
2 results on '"DNA, Protozoan cerebrospinal fluid"'

1. Evaluation of different techniques in the diagnosis of Toxoplasma encephalitis.

Author

Rodríguez JC, Martínez MM, Martínez AR, and Royo G

Subjects
Adult, Animals, Antibody Affinity, Antigens, Protozoan blood, Antigens, Protozoan cerebrospinal fluid, Cerebrospinal Fluid parasitology, DNA, Protozoan cerebrospinal fluid, Encephalitis diagnosis, Enzyme-Linked Immunosorbent Assay, Evaluation Studies as Topic, Humans, Immunoglobulin A blood, Immunoglobulin G blood, Immunoglobulin G immunology, Immunoglobulin M blood, Sensitivity and Specificity, Toxoplasma genetics, Toxoplasma isolation & purification, AIDS-Related Opportunistic Infections diagnosis, Antibodies, Protozoan blood, Antigens, Protozoan analysis, Polymerase Chain Reaction, Toxoplasma immunology, Toxoplasmosis, Cerebral diagnosis
Abstract

This study evaluated the detection of antibodies, circulating antigens and parasite DNA by polymerase chain reaction (PCR) in the diagnosis of toxoplasma encephalitis. The detection of antibody classes and IgG avidity were not useful diagnostically. The detection of circulating antigens by the ELISA system described was not sufficiently sensitive. The detection of DNA by PCR was the most useful test especially in untreated patients, with a sensitivity of 62% overall, 81% in untreated patients and only 20% in treated patients. The use of non-isotopic probes makes the use of this technique feasible in routine diagnostic parasitology laboratories.

Published
1997
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2. PCR detection of Toxoplasma gondii DNA in CSF for the differential diagnosis of AIDS-related focal brain lesions.

Author

Cingolani A, De Luca A, Ammassari A, Murri R, Linzalone A, Grillo R, and Antinori A

Subjects
AIDS-Related Opportunistic Infections drug therapy, Adult, Animals, Antibodies, Protozoan blood, Coccidiostats therapeutic use, Cohort Studies, Confidence Intervals, Diagnosis, Differential, Female, Humans, Immunoglobulin G blood, Male, Middle Aged, Odds Ratio, Prospective Studies, Sensitivity and Specificity, Spinal Puncture, Toxoplasma genetics, Toxoplasma immunology, Toxoplasmosis, Cerebral drug therapy, AIDS-Related Opportunistic Infections diagnosis, DNA, Protozoan cerebrospinal fluid, Polymerase Chain Reaction, Toxoplasma isolation & purification, Toxoplasmosis, Cerebral diagnosis
Abstract

To evaluate whether the detection of Toxoplasma gondii DNA in CSF could contribute to the differential diagnosis of AIDS-related focal brain lesions, CSF samples from 88 HIV-infected patients (56 with focal brain lesions and 32 without) were tested prospectively by a nested PCR for the B1 gene of T. gondii. The assay had a detection limit of 10 trophozoite equivalents. Six of 18 patients with toxoplasmic encephalitis, but none of the 70 patients with other disorders, were PCR-positive (33.3% sensitivity and 100% specificity). Considering only those patients with cerebral toxoplasmosis from whom CSF was collected before or during the first week of antitoxoplasmic therapy, sensitivity rose to 50%. This was higher than the sensitivity in patients whose CSF was collected after the first week of treatment (odds ratio (OR) of 7.0; 95% CI: 0.46-218.2). The administration of antitoxoplasmic prophylaxis did not affect the PCR results. Patients with a poor response to therapy had a higher probability of detectable T. gondii DNA in their CSF (OR of 5.0; 95% CI: 0.37-86.6). All patients with other central nervous system disorders were PCR-negative. Despite the moderate sensitivity, the high specificity and positive predictive value (100%) make this assay a useful tool in the differential diagnosis of AIDS-related focal brain lesions as part of a series of CSF and neuroradiological examinations.

Published
1996
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