1. Usefulness of antimicrobial resistance pattern for detecting PVL- or TSST-1-producing meticillin-resistant Staphylococcus aureus in a French university hospital
- Author
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Daniel Talon, Xavier Bertrand, Houssein Gbaguidi-Haore, Michelle Thouverez, Gérard Couetdic, Pascal Cholley, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
- Subjects
MESH : Leukocidins ,Male ,MESH : Retrospective Studies ,MESH : Aged ,MESH: Leukocidins ,Hospitals, University ,0302 clinical medicine ,MESH : Child ,MESH: Superantigens ,MESH: Child ,MESH : Superantigens ,Child ,Aged, 80 and over ,0303 health sciences ,Cross Infection ,MESH: Middle Aged ,Superantigens ,MESH : Genes, Bacterial ,MESH : Infant ,[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie ,General Medicine ,Staphylococcal Infections ,Antimicrobial ,MESH: Infant ,3. Good health ,Electrophoresis, Gel, Pulsed-Field ,MESH : Phenotype ,MESH: Electrophoresis, Gel, Pulsed-Field ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Staphylococcus aureus ,MESH: Young Adult ,Child, Preschool ,MESH : Cross Infection ,Microbiology (medical) ,Methicillin-Resistant Staphylococcus aureus ,Fusidic acid ,MESH : Molecular Epidemiology ,MESH: Staphylococcal Infections ,MESH : Young Adult ,Exotoxins ,MESH: Methicillin-Resistant Staphylococcus aureus ,Microbial Sensitivity Tests ,MESH: Phenotype ,Microbiology ,03 medical and health sciences ,Antibiotic resistance ,MESH : Adolescent ,MESH: Molecular Epidemiology ,MESH : Methicillin-Resistant Staphylococcus aureus ,Humans ,MESH : Middle Aged ,MESH : Aged, 80 and over ,MESH : Hospitals, University ,MESH : Electrophoresis, Gel, Pulsed-Field ,Aged ,Retrospective Studies ,MESH: Adolescent ,MESH: Humans ,MESH: Methicillin Resistance ,030306 microbiology ,MESH: Child, Preschool ,MESH : Humans ,Infant ,MESH: Adult ,MESH: Retrospective Studies ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Virology ,MESH : Methicillin Resistance ,MESH : Exotoxins ,MESH : Staphylococcal Infections ,MESH: Female ,Meticillin ,Drug resistance ,MESH : Child, Preschool ,medicine.disease_cause ,Enterotoxins ,MESH: Aged, 80 and over ,[ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology ,Leukocidins ,MESH: Enterotoxins ,MESH : Female ,030212 general & internal medicine ,MESH : Enterotoxins ,MESH: Aged ,MESH: Microbial Sensitivity Tests ,Molecular Epidemiology ,MESH: Infant, Newborn ,MESH : Adult ,Middle Aged ,Phenotype ,Female ,France ,MESH: Genes, Bacterial ,medicine.drug ,Adult ,Adolescent ,MESH : Male ,Bacterial Toxins ,MESH : Infant, Newborn ,Young Adult ,medicine ,MESH : France ,MESH: Hospitals, University ,business.industry ,Infant, Newborn ,MESH: Cross Infection ,MESH: Exotoxins ,MESH: Male ,Multiple drug resistance ,MESH: France ,MESH: Bacterial Toxins ,Genes, Bacterial ,MESH : Bacterial Toxins ,Multilocus sequence typing ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Methicillin Resistance ,MESH : Microbial Sensitivity Tests ,business - Abstract
Several recent reports have suggested that community-associated meticillin-resistant Staphylococcus aureus (MRSA) clones, particularly those harbouring genes for Panton–Valentine leukocidin (PVL) or toxic shock syndrome toxin 1 (TSST-1), are increasingly responsible for infections in hospitals. Here, a retrospective study was carried out to investigate whether antimicrobial resistance patterns could be used to detect these pathogens in a French university hospital. Isolates were characterized by antimicrobial susceptibility testing, PCR profiling (PVL genes and tst), PFGE typing and multilocus sequence typing. Demographic and clinical data were collected from all patients. For PVL-positive MRSA, the typical antimicrobial resistance pattern (susceptible to fluoroquinolones, non-susceptible to fusidic acid, kanamycin resistant and susceptible to gentamicin and tobramycin) had a sensitivity of 77.8 % and a positive predictive value (PPV) of 100 %. For tst-positive MRSA, the antimicrobial resistance pattern (susceptible to fluoroquinolones and non-susceptible to fusidic acid) had a sensitivity of 100 % and a PPV of 72.4 %. These results suggest that phenotypic rules based on antimicrobial resistance patterns are potentially useful for the detection of PVL- and tst-positive MRSA isolates.
- Published
- 2009