8 results on '"Dario Di Luca"'
Search Results
2. Human herpesvirus-6 modulates RANTES production in primary human endothelial cell cultures
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Arnaldo Caruso, Giulio Alessandri, Manuela Grassi, Flavia Favilli, Antonella Rotola, Manola Comar, Dario Di Luca, Simona Fiorentini, and Douglas Horejsh
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Umbilical Veins ,Chemokine ,Cell type ,Time Factors ,Ultraviolet Rays ,Herpesvirus 6, Human ,Aorta, Thoracic ,Biology ,medicine.disease_cause ,Herpesviridae ,Virus ,Chemokine receptor ,Virology ,medicine ,Humans ,RNA, Messenger ,Chemokine CCL5 ,Cells, Cultured ,Aged ,Middle Aged ,biology.organism_classification ,Coronary Vessels ,Endothelial stem cell ,Infectious Diseases ,Cell culture ,biology.protein ,RNA, Viral ,Receptors, Virus ,Receptors, Chemokine ,Human herpesvirus 6 ,Endothelium, Vascular - Abstract
U.C.O. Hygien Institute, University of Trieste, IRRCS Burlo Garofalo, Trieste, ItalyHuman herpesvirus 6 (HHV6) is a beta-herpes-virus capable of infecting several cell types fromdifferent origins. HHV6 is the etiological agent ofexantem subitum and has been associated withseveral diseases, all characterized by an inflam-matory response triggered by chemokines. Weshow that strain U1102 of HHV6 is able to infectpersistently human endothelial cells obtainedfrom umbilical veins, adult aorta and adult heartmicrovessels,withoutapparentcytopathiceffect.Analysis by in situ PCR showed that HHV6sequences were present in 20% of HUVEC, 10%ofaortic,and1%ofheartmicrovascularendothe-lial cells. Regardless of endothelial cell origin,HHV6 infection induced de novo synthesis of theRANTES CC-chemokine. It was found, however,thatmicrovascularendothelialcells,despitetheirlower susceptibility to HHV6 infection, showedthe highest RANTES expression. Chemokineproduction occurred also in the absence of viralDNA synthesis. Furthermore, RANTES synthesisrequired an active viral genome, as UV-inacti-vated HHV6 infection of endothelial cells did notlead to chemokine production. We investigatedtheexpressionofHHV6U51gene,whichencodesa chemokine receptor that is already known tosequesteranddownmodulateRANTESinepithe-lial cells. HHV6-infected endothelial cells co-expressed RANTES and U51 mRNAs startingfrom 12 hr up to 48 hr post-infection. Then,RANTES transcripts disappeared whereas U51messages continued to be expressed. In con-clusion, this study highlights the major role ofHHV6 in endothelial cell biology and the devel-opment of inflammatory processes. J. Med.Virol.70:451–458,2003.
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- 2003
3. HHV-6 infects human aortic and heart microvascular endothelial cells, increasing their ability to secrete proinflammatory chemokines
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Manuela Grassi, Giulio Alessandri, Cesare Campello, Enzo Cassai, Monica Galvan, Maria Tosetti, Arnaldo Caruso, Elisabetta Caselli, Flavia Favilli, Antonella Rotola, Emirena Garrafa, Dario Di Luca, Manola Comar, Caruso, A, Rotola, A, Comar, Manola, Favilli, F, Galvan, M, Tosetti, M, Campello, Cesare, Caselli, E, Alessandri, G, Grassi, M, Garrafa, E, Cassai, E, and DI LUCA, D.
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Pathology ,medicine.medical_specialty ,Chemokine ,endothelium ,Endothelium ,Herpesvirus 6, Human ,viruses ,medicine.medical_treatment ,Inflammation ,NO ,Cell Line ,Proinflammatory cytokine ,herpesvirus ,inflammation ,MCP-1 ,IL-8 ,Virology ,Leukocytes ,medicine ,Humans ,Interleukin 8 ,Aorta ,Chemokine CCL2 ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Monocyte ,Interleukin-8 ,virus diseases ,Heart ,Herpesviridae Infections ,Endothelial stem cell ,Infectious Diseases ,Cytokine ,medicine.anatomical_structure ,Immunology ,biology.protein ,RNA, Viral ,Endothelium, Vascular ,Chemokines ,medicine.symptom - Abstract
Endothelial cells are important targets for herpesvirus infection. To evaluate the biological effects of human herpesvirus-6 (HHV-6) infection, adult heart microvascular and aortic endothelial cells were examined for in vitro susceptibility to HHV-6 and for the alterations induced by viral infection on the production of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8). Analysis by reverse transcription-polymerase chain reaction and by in situ polymerase chain reaction showed that HHV-6 replicates in endothelium in the absence of cytopathic effects, and that viral sequences were present in 20% umbilical vein and in 10% aortic and 1% microvascular endothelium. HHV-6 infection upregulated the production of MCP-1 and IL-8, with differences observed between aortic and microvascular endothelium. These findings demonstrate that endothelial cells represent a potential reservoir for HHV-6 infection, and the altered pattern of chemokine production can lead to attraction of immunocompetent cells and to the development of inflammatory processes.
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- 2002
4. Human herpesvirus 7 is latent in gastric mucosa
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C. Rizzo, Patrizia Querzoli, Alessandro Pozzoli, Michela Boni, Enzo Cassai, Dario Di Luca, S. Boccia, and Arianna Gonelli
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Human herpesvirus 7 ,Biopsy ,viruses ,Chronic gastritis ,Herpesvirus 7, Human ,medicine.disease_cause ,Herpesviridae ,NO ,Virology ,medicine ,Gastric mucosa ,Humans ,Gammaherpesvirinae ,Helicobacter pylori ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Stomach ,virus diseases ,Viral Load ,biology.organism_classification ,medicine.disease ,Virus Latency ,Infectious Diseases ,medicine.anatomical_structure ,Gastric Mucosa ,Gastritis ,Chronic Disease ,DNA, Viral ,Immunology ,Leukocytes, Mononuclear ,medicine.symptom ,Viral load ,Follow-Up Studies - Abstract
Chronic gastritis is associated frequently with persistent infection by Helicobacter pylori. However, not all patients with chronic gastritis have evidence of H. pylori infection, suggesting that other factors might contribute to the development of gastritis. The present study was undertaken to evaluate a possible etiologic role of human herpesvirus 7 (HHV-7). HHV-7 DNA was detected in about 80% of gastric biopsies, both in healthy mucosa from individuals without evidence of inflammation and in biopsies from patients with histologically confirmed chronic gastric inflammation. HHV-7 was present also in H. pylori negative samples, was associated specifically with gastric tissue and not with residual blood within the mucosa, and was present with high viral loads. HHV-7 DNA persisted in several patients also after remission of gastric inflammation and the viral presence did not correlate with specific symptoms. Analysis by RT-PCR showed that HHV-7 is transcriptionally inactive in chronic gastritis lesions. These observations show that gastric tissue represents a site of HHV-7 latent infection and a potential reservoir for viral reactivation.
- Published
- 2001
5. Human herpesvirus 6 in human immunodeficiency virus-infected individuals: Association with early histologic phases of lymphadenopathy syndrome but not with malignant lymphoproliferative disorders
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Laura Sighinolfi, Emanuela Vaccher, Umberto Tirelli, Annunziata Gloghini, Dario Di Luca, Antonino Carbone, Riccardo Dolcetti, Salvatore De Vita, Enzo Cassai, Prisco Mirandola, and Mauro Boiocchi
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Herpesvirus 4, Human ,Herpesvirus 6, Human ,medicine.medical_treatment ,AIDS-related complex ,Lymphoproliferative disorders ,HIV Infections ,HHV-6 ,HIV ,lymphoadenopathy ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Asymptomatic ,Herpesviridae ,Virus ,AIDS-Related Complex ,Virology ,medicine ,Humans ,AIDS-Related Opportunistic Infections ,virus diseases ,Immunosuppression ,Herpesviridae Infections ,medicine.disease ,biology.organism_classification ,Lymphoproliferative Disorders ,Lymphoma ,Infectious Diseases ,DNA, Viral ,Immunology ,Human herpesvirus 6 ,medicine.symptom - Abstract
Preliminary evidence suggested that human herpesvirus-6 (HHV-6) may act as a cofactor in acquired immunodeficiency syndrome (AIDS) and may contribute to the pathogenesis of lymphoproliferative disorders occurring in individuals infected with the human immunodeficiency virus (HIV). To understand better the biological and clinical significance of HHV-6 infection in the context of HIV-related immunosuppression, the polymerase chain reaction was used to study the frequency and variant distribution of HHV-6 in peripheral blood mononucleated cells (PBMCs) from HIV-seropositive individuals, either asymptomatic or with lymphadenopathy syndrome (LAS) or with overt AIDS. Non-neoplastic and malignant lymphoproliferative disorders from both HIV-infected and HIV-seronegative patients were also investigated using the same series of samples for the presence of Epstein-Barr virus (EBV). When compared with healthy blood donors (12/42, 29%), HHV-6 prevalence in PBMCs showed a progressive decline in HIV-seropositive individuals with asymptomatic HIV infection (3/26, 11%) and in patients with LAS (1/13, 8%) and a significant reduction in patients with overt AIDS (1/20, 20%; P = 0.02). The decrease correlated with the number of CD4+ cells at the time of examination. In addition, HHV-6 DNA sequences were significantly more prevalent in LAS biopsies (13/20, 65%) than in HIV-unrelated reactive lymphadenopathies (2/10, 20%; P = 0.02) and the presence of HHV-6 sequences correlated closely with a histologic pattern of follicular hyperplasia (13/16, 81%; P = 0.003). Strikingly, HHV-6 prevalence decreased in PBMCs of LAS patients, suggesting that the likelihood of interactions between HHV-6 and HIV varies in different body districts. In particular, the demonstration that all HHV-6-carrying LAS samples were also positive for HIV infection suggests that LAS lymph nodes constitute one of the sites where biologically relevant interactions between the two viruses might occur. Also, the prevalence of EBV was higher in LAS (14/20, 70%) than in non-neoplastic lymph nodes from HIV-seronegative individuals (4/10, 40%), although the difference was not statistically significant. EBV was associated strongly with HIV-related malignant lymphoproliferative disorders, being detected in 100% of patients with Hodgkin's disease (HD) and 53% of B-cell non-Hodgkin's lymphomas (NHL). In contrast, the prevalence of HHV-6 DNA in HD and B-cell NHL arisen in HIV-infected patients (30% and 6% respectively) was remarkably lower and similar to that observed in lymphoproliferative disorders from HIV-seronegative patients. Finally, as observed in healthy individuals, HHV-6 variant B was more prevalent than variant A in benign and malignant lymphoproliferative disorders from bot HIV-infected and HIV-seronegative patients. These results suggest that the interactions between HHV-6 and HIV could be different in the various phases of HIV disease and in different districts; HHV-6 has probably no direct role in the pathogenesis of HIV-associated B-cell NHL and HD cases, and behave differently from EBV; and HIV-related immunosuppression does not alter the distribution of HHV-6 variants in these tissues, as observed in the case of EBV.
- Published
- 1996
6. Human herpesviruses 6 and 7 in salivary glands and shedding in saliva of healthy and human immunodeficiency virus positive individuals
- Author
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Enzo Cassai, A Frigatti, Prisco Mirandola, Laura Sighinolfi, Tullia Ravaioli, Pasqualina Bovenzi, Dario Di Luca, Riccardo Dolcetti, and Paolo Monini
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Saliva ,Herpesvirus 6, Human ,viruses ,Molecular Sequence Data ,Common Cold ,Herpesvirus 7, Human ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Salivary Glands ,Herpesviridae ,Virus ,stomatognathic system ,Virology ,HIV Seropositivity ,medicine ,Humans ,Viral shedding ,Base Sequence ,Salivary gland ,virus diseases ,Cytomegalovirus ,Herpesviridae Infections ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Virus Shedding ,Infectious Diseases ,medicine.anatomical_structure ,Case-Control Studies ,DNA, Viral ,Immunology ,Stomatitis, Aphthous ,Human herpesvirus 6 ,Viral load - Abstract
The presence of human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) was investigated by the polymerase chain reaction in saliva specimens from healthy persons, donors affected by common cold or recurrent aphthous ulceration (RAU), and human immunodeficiency virus (HIV) positive patients, and in salivary gland biopsies. The sensitivity of the technique made it possible to detect as few as 5-10 target molecules in 15 microliters of saliva. HHV-6 was present in 63% of salivary gland biopsies and in 3% of salivas from healthy persons. No significant difference in the presence of HHV-6 was detected in specimens from donors with common cold, RAU, or HIV-infected patients. HHV-7 was present in 75% of salivary glands and in 55% of salivas from healthy persons. HHV-7 was detected with similar frequency in salivas from donors with common cold or RAU. Salivas from HIV-infected patients harbored HHV-7 with higher frequency (81%) and increased viral load. These results show that salivary glands are a site of persistent infection for both HHV-6 and HHV-7. However, the two viruses seem to differ in their biological properties: 1) HHV-6 is rarely present in saliva in detectable amounts, while HHV-7 is frequently detected; and 2) immunosuppression by acquired immunodeficiency syndrome (AIDS) increases the frequency of detection and the viral load of HHV-7, but does not have a significant effect on HHV-6 shedding in saliva.
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- 1995
7. Isolation of human herpesvirus 7 from an infant with febrile syndrome
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Prisco Mirandola, Claudio Cermelli, Marinella Portolani, and Dario Di Luca
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Virus Cultivation ,Fever ,viruses ,Herpesvirus 7, Human ,medicine.disease_cause ,Polymerase Chain Reaction ,Herpesviridae ,Virus ,law.invention ,law ,Virology ,Alphaherpesvirinae ,medicine ,Humans ,Polymerase chain reaction ,biology ,virus diseases ,Infant ,Herpesviridae Infections ,Syndrome ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Human genetics ,Infectious Diseases ,Genetic marker ,Immunology ,DNA, Viral ,Human herpesvirus 6 ,Viral disease - Abstract
A viral isolate obtained from peripheral blood lymphocytes of an infant with a nonspecific febrile syndrome was identified as human herpesvirus 7 (HHV-7) on the basis of PCR analysis of its DNA with one set of primers specific for HHV-7. The correlation of HHV-7 with the febrile episode affecting the infant is suggested.
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- 1995
8. Is vestibular papillomatosis associated with human papillomavirus?
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Silvano Costa, Paola Secchiero, Patrizia Masotti, Enzo Cassai, Dario Di Luca, Antonella Rotola, Patrizia Terzano, Maria G. Poggi, and Giuseppe Martinell
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Sexually transmitted disease ,Adult ,Pathology ,medicine.medical_specialty ,Vestibular papillomatosis ,Papillomatosis ,Biology ,Vulva ,Virology ,medicine ,Humans ,Clinical significance ,Papillomaviridae ,Southern blot ,Vestibular system ,HPV infection ,Middle Aged ,medicine.disease ,Tumor Virus Infections ,Infectious Diseases ,medicine.anatomical_structure ,DNA, Viral ,Female ,Vulvar Diseases ,medicine.symptom ,DNA Probes ,Follow-Up Studies - Abstract
The origin and clinical significance of vestibular papillae were evaluated by comparing histological features with the presence of human papillomavirus (HPV) types 6/11 and 16/18, as revealed by Southern blot DNA hybridization. Twenty women with vestibular papillomatosis underwent clinical evaluation and follow-up. When available, male partners were also examined. Histological changes suggestive of HPV infection were present in all the 20 specimens. Sixteen cases (80%) contained DNA sequences homologous to the viral probes. In particular, 12 cases (60%) reacted with the HPV 16/18 probe. Follow-up for more than 18 months revealed no variation in the distribution and appearance of vestibular papillae. No male partner showed signs of HPV lesions. The study shows that HPV 16 is frequently associated with vestibular papillae but does not support a productive infection. Therefore the most appropriate management of these patients should be evaluated clinically in each individual case.
- Published
- 1991
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